Short communication. Strain heterogeneity in Mycoplasma pullorum isolates identified by random amplified polymorphic DNA techniques

  • E. Lobo National Center Animal and Plant Health (CENSA). San José de Las Lajas. La Habana
  • M.C. García Poultry Diagnostic and Research Centre. University of Georgia.Medicine.. Athens, Georgia
  • H. Moscoso Poultry Diagnostic and Research Centre. University of Georgia. Medicine.. Athens, Georgia
  • S. Martínez National Centre for Animal and Plant Health. San José de las Lajas La Habana
  • S.H. Kleven Poultry Diagnostic and Research Centre. University of Georgia.Medicine.. Athens, Georgia
DOI: https://doi.org/10.5424/sjar/2004024-116
Keywords: CHICKENS, MYCOPLASMOSES, MYCOPLASMA, GENETIC VARIATION, IDENTIFICATION, RAPD, EPIDEMIOLOGY, CUBA

Abstract

Mycoplasmas were isolated from chickens with respiratory problems during field investigations of a concentrated respiratory disease outbreak in western Cuba, 1997. A high percentage of mycoplasma cultures from tracheas and air-sac lesions yielded pure cultures of Mycoplasma pullorum. The aim of the present work was to investigate the heterogeneity among M. pullorum isolates from Cuba and strains from other countries using random amplified polymorphic DNA (RAPD) techniques. The results show that the RAPD method may be a useful identification tool for studying the epidemiology of poultry mycoplasmosis in Cuba.

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Published
2004-12-01
How to Cite
Lobo, E., García, M., Moscoso, H., Martínez, S., & Kleven, S. (2004). Short communication. Strain heterogeneity in Mycoplasma pullorum isolates identified by random amplified polymorphic DNA techniques. Spanish Journal of Agricultural Research, 2(4), 500-503. https://doi.org/10.5424/sjar/2004024-116
Issue
Vol. 2 No. 4 (2004)
Section
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