Mitigation of salinity stress in canola plants by sodium nitroprusside application

  • Saad Farouk Mansoura University, Fac. of Agriculture, Agricultural Botany Dept., Mansoura 35516
  • Sally A. Arafa Mansoura University, Fac. of Agriculture, Agricultural Botany Dept., Mansoura 35516
Keywords: antioxidants, Brassica napus L., osmoprotectants, ultrastructure, yield

Abstract

Salinity is a global issue threatening land productivity and food production. The present study aimed to examine the role of sodium nitroprusside (SNP) on the alleviation of NaCl stress on different parameters of canola (Brassica napus L.) plant growth, yield as well as its physiological and anatomical characteristics. Canola plants were grown under greenhouse conditions in plastic pots and were exposed to 100 mM NaCl. At 50 and 70 days from sown, plants were sprayed with SNP (50 and 100 µM) solutions under normal or salinity condition. Growth and yield characters as well as some biochemical and anatomical changes were investigated under the experimental conditions. Salinity stress caused an extremely vital decline in plant growth and yield components. A significant increase was found in membrane permeability, lipid peroxidation, hydrogen peroxide, sodium, chloride, proline, soluble sugars, ascorbic and phenol in canola plants under salinity stress. Under normal conditions, SNP application significantly increased all studies characters, except sodium, chloride, hydrogen peroxide, lipid peroxidation, membrane permeability that markedly reduced. Application of SNP to salt-affected plants mitigated the injuries of salinity on plant growth, yield, and improved anatomical changes. The present investigation demonstrated that SNP has the potential to alleviate the salinity injurious on canola plants.

Downloads

Download data is not yet available.

References

Ahmad P, Ahanger MA, Alyemeni MN, Wijaya L, Alam P, Ashraf M, 2018. Mitigation of sodium chloride toxicity in Solanum lycopersicum L. by supplementation of jasmonic acid and nitric oxide. J Plant Interact 13 (1): 64-72. https://doi.org/10.1080/17429145.2017.1420830

Akladious SA, Mohamed HI, 2017. Physiological role of exogenous nitric oxide in improving performance yield and some biochemical aspects of sunflower plant under zinc stress. Acta Biologica Hungarica 68 (1): 101-114. https://doi.org/10.1556/018.68.2017.1.9

Akram N, Iqbal M, Muhammad A, Ashraf M, Al-Qurainy F, Shafiq S, 2018. Aminolevulinic acid and nitric oxide regulate oxidative defense and secondary metabolisms in canola (Brassica napus L.) under drought stress. Protoplasma 225: 163-174. https://doi.org/10.1007/s00709-017-1140-x

AOAC, 1990. Official methods of analysis, association of official analytical chemists, 15th AOAC, Washington, DC. USA.

Arbona V, Flors V, Jacas J, García-Agustín P, Gómez-Cadenas A, 2003. Enzymatic and non-enzymatic antioxidant responses of Carrizo citrange, a salt sensitive citrus rootstock, to different levels of salinity. Plant Cell Physiol 44: 388-394. https://doi.org/10.1093/pcp/pcg059

Atkin OK, Macherel D, 2009. The crucial role of plant mitochondria in orchestrating drought tolerance. Ann Bot 103: 581-597. https://doi.org/10.1093/aob/mcn094

Austin JR, Frost E, Vidi PA, Kessler F, Staehelin LA, 2006. Plastoglobules are lipoprotein subcompartments of the chloroplast that are permanently coupled to thylakoid membranes and contain biosynthetic enzymes. Plant Cell 18: 1693-1703. https://doi.org/10.1105/tpc.105.039859

Banon SJ, Ochoa J, Franco JA, Alarcon JJ, Sanchez-Blanco M, 2006. Hardening of oleander seedlings by deficit irrigation and low air humidity. Environ Exp Bot 56: 36-43. https://doi.org/10.1016/j.envexpbot.2004.12.004

Beligni MV, Lamattina L, 2002. Nitric oxide interferes with plant photo-oxidative stress by detoxifying reactive oxygen species. Plant Cell Environ 25: 737-748. https://doi.org/10.1046/j.1365-3040.2002.00857.x

Blokhina O, Violainen E, Agerstedt KV, 2003. Antioxidants, oxidative damage and oxygen deprivation stress: A review. Ann Bot 91: 179-194. https://doi.org/10.1093/aob/mcf118

Bose J, Rodrigo-Moreno A, Lai D, Xie Y, Shen W, Shabala S, 2015. Rapid regulation 533 of the plasma membrane H+-ATPase activity is essential to salinity tolerance in two 534 halophyte species Atriplex lentiformis and Chenopodium quinoa. Ann Bot 115: 481-494. https://doi.org/10.1093/aob/mcu219

Chaudhary MT, Wainwright SJ, Merrett MJ, 1996. Comparative NaCl tolerance of Lucerne plants regenerated from salt-selected suspension cultures. Plant Sci 114 (2): 221-232. https://doi.org/10.1016/0168-9452(96)04326-9

Chrominski A, Halls S, Weber DJ, Smith BN, 1989. Proline affects ACC to ethylene conversion under salt and water stresses in the halophyte, Allenrolfea occidentalis. Envir Exp Botany 29 (3): 359-363. https://doi.org/10.1016/0098-8472(89)90010-5

FAO, 2014. FAO land and plant nutrition management service. http://www.fao.org/publications/en/

FAOSTAT, 2011. Egypt 2011. http://faostat.fao.org/site/666/default.aspx

Fatma M, Asgher M, Masood A, Khan NA, 2014. Excess sulfur supplementation improves photosynthesis and growth in mustard under salt stress through increased production of glutathione. Environ Exp Bot 107: 55-63. https://doi.org/10.1016/j.envexpbot.2014.05.008

Fayez KA, Bazaid SA, 2014. Improving drought and salinity tolerance in barley by application of salicylic acid and potassium nitrate. J Saudi Soc Agric Sci 13: 45-55.

Fernandez-Ballester G, Martinez V, Ruiz D, Cerda A, 1998. Changes in inorganic and organic solutes in Citrus growing under saline stresses. J Plant Nut 21 (12): 2497-2514. https://doi.org/10.1080/01904169809365582

Franco D, Sineiro J, Rubilar M, Sanchez M, Jerez M, 2008. Polyphenols from plant materials: Extraction and antioxidant power. Electr J Envir Agr Food Chem 7: 3210-3216.

Ghadakchiasl A, Mozafari AA, Ghaderi N, 2017. Mitigation by sodium nitroprusside of the effects of salinity on the morpho-physiological and biochemical characteristics of Rubus idaeus under in vitro conditions. Physiol Mol Biol Plants 23 (1): 73-83. https://doi.org/10.1007/s12298-016-0396-5

Gill SS, Hasanuzzaman M, Nahar K, Macovei A, Tuteja N, 2013. Importance of nitric oxide in cadmium stress tolerance in crop plants. Plant Physiol Biochem 63: 254-261. https://doi.org/10.1016/j.plaphy.2012.12.001

Goncalves JF, Becker AG, Cargnelutti D, Tabaldi LA, Pereira LB, Battisti V, Spanevello R, Morch VM, Nicoloso FT, Schetinger MRC, 2007. Cadmium toxicity causes oxidative stress and induces response to the antioxidant system in cucumber seedlings. Braz J Plant Physiol 19: 24-26. https://doi.org/10.1590/S1677-04202007000300006

Gong DH, Wang GZ, Si WT, Zhou Y, Liu Z, Jia J, 2018. Effect of salt stress on photosynthetic pigments and activity of ribulose-1,5-bisphosphate carboxylase/oxygenase in Kalidium foliatum. Russ J Plant Physiol 65 (1): 98-103. https://doi.org/10.1134/S1021443718010144

Graß F, Durner J, Gaupels F, 2013. Nitric oxide, antioxidants and prooxidants in plant defense responses. Front Plant Sci 29 (4): 419.

Gupta P, Srivastava S, Shekhar Seth C, 2017. 24-epibrassinolide and sodium nitroprusside alleviate the salinity stress in Brassica juncea L. cv. Varuna through cross talk among proline, nitrogen metabolism and abscisic acid. Plant Soil 411: 483-498. https://doi.org/10.1007/s11104-016-3043-6

Hanafy AAH, Mohamed Hanaa FY, Orabi IOA, EL-Hefny AM, 2018. Influence of gamma rays, humic acid and sodium nitroprusside on growth, chemical constituents and fruit quality of snap bean plants under different soil salinity levels. Biosci Res 15 (2): 575-588.

Hasanuzzaman M, Nahar K, Rahman A, Inafuku M, Oku H, Fujits M, 2018. Exogenous nitric oxide donor and arginine provide protection against short-term drought stress in wheat seedlings. Physiol Mol Biol Plant, in press. https://doi.org/10.1007/s12298-018-0531-6

Hassan MB, Shafique FA, 2011. Current situation of edible vegetable oils and some propositions to curb the oil gap in Egypt. Nature Sci 8: 1-7.

Julkenen-Titto R, 1985. Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics. J Agric Food Chem 33: 213-217. https://doi.org/10.1021/jf00062a013

Kausar F, Shahbaz M, 2013. Interactive effect of foliar application of nitric oxide (NO) and salinity on wheat (Triticum aestivum L.). Pak J Bot 45: 67-73.

Kaya C, Akram NA, Ashraf M, Sonmez O, 2018. Exogenous application of humic acid mitigates salinity stress in maize (Zea mays L.) plants by improving some key physio-biochemical attributes. Cereal Res Commun 46 (1): 67-78. https://doi.org/10.1556/0806.45.2017.064

Kaya C, Sonmez O, Aydemir S, Ashraf M, Dikilitas M, 2013. Exogenous application of mannitol and thiourea regulates plant growth and oxidative stress responses in salt-stressed maize (Zea mays L.). J Plant Interact 8: 234-241. https://doi.org/10.1080/17429145.2012.725480

Khatun S, Flowers TJ, 1995. Effects of salinity on seed set in rice. Plant Cell Environ 18 (1): 61-67. https://doi.org/10.1111/j.1365-3040.1995.tb00544.x

Kholghi M, Toorchi M, Bandeh-Hagh A, Shakiba MR, 2018. An evaluation of canola genotypes under salinity stress at vegetative stage via morphological and physiological traits. Pak J Bot 50 (2): 447-455.

Lichtenthaler HK, Wellburn AR, 1985. Determination of total carotenoids and chlorophylls A and B of leaf in different solvents. Biol Soc Trans 11: 591-592. https://doi.org/10.1042/bst0110591

Naeem MS, Warusawitharana H, Liu H, Ahmad R, Waraich EA, Xu L, Zhou W, 2012. 5-aminolevulinic acid alleviates the salinity-induced changes in Brassica napus as revealed by the ultrastructural study of chloroplast. Plant Physiol Bioch 57: 84-92. https://doi.org/10.1016/j.plaphy.2012.05.018

Nasrin AB, Anamul H, Megumi WS, Mohammad MI, Misugi U, Ken M, Yoshimasa N, Yoshiyuki M, 2010. Proline and glycine betaine ameliorated NaCl stress via scavenging of hydrogen peroxide and methyl glyoxal but not superoxide or nitric oxide in tobacco cultured cells. Biosci Biotechnol Biochem 74: 2043-2049. https://doi.org/10.1271/bbb.100334

Nazar R, Khan MIR, Iqbal N, Masood A, Khan NA, 2014. Involvement of ethylene in reversal of salt-inhibited photosynthesis by sulfur in mustard. Physiol Plant 152: 331-344. https://doi.org/10.1111/ppl.12173

Rahmani F, Peymani A, Gorttapeh AH, 2018. Comparison of biochemical and molecular responses of two Brassica napus L. cultivars differing in drought tolerance to salt stress. Ind J Plant Physiol 23 (1): 48-56. https://doi.org/10.1007/s40502-017-0323-y

Rai KK, Rai N, Rai SP, 2018. Salicylic acid and nitric oxide alleviate high temperature induced oxidative damage in Lablab purpureus L plants by regulating bio-physical processes and DNA methylation. Plant Physiol Biochem 128: 72-88. https://doi.org/10.1016/j.plaphy.2018.04.023

Rao MV, Paliyath G, Ormrod DP, Murr DP, Watkins CB, 1997. Influence of salicylic acid on H2O2 production, oxidative stress and H2O2-metabolizing enzymes: salicylic acid-mediated oxidative damage requires H2O2. Plant Physiol 115: 137-149. https://doi.org/10.1104/pp.115.1.137

Reddy PS, Jogeswar G, Rasineni GK, Maheswari M, Reddy AR, Varshney RK, 2015. Proline over-accumulation alleviates salt stress and protects photosynthetic and antioxidant enzyme activities in transgenic sorghum (Sorghum bicolor (L.) Moench). Plant Physiol Biochem 94: 104-113. https://doi.org/10.1016/j.plaphy.2015.05.014

Rizhsky L, Liang H, Shuman J, Shulaev V, Davletova S, Mittler R, 2004. When defense pathways collide. The response of Arabidopsis to a combination of drought and heat stress. Plant Physiol 134: 1683-1696. https://doi.org/10.1104/pp.103.033431

Sadasivam S, Manickam S, 1996. Biochemical methods, 2nd ed., New Age Int Ltd Publ, India.

Santos CV, 2004. Regulation of chlorophyll biosynthesis and degradation by salt stress in sunflower leaves. Sci Hort 103: 93-99. https://doi.org/10.1016/j.scienta.2004.04.009

Shafiq S, Akram NA, Ashraf M, Arshad A, 2014. Synergistic effects of drought and ascorbic acid on growth, mineral nutrients and oxidative defense system in canola (Brassica napus L.) plants. Acta Physiol Plant 36: 1539-1553. https://doi.org/10.1007/s11738-014-1530-z

Shao HB, Liang ZS, Shao MA, 2005. Change of antioxidative enzymes and MDA among 10 wheat genotypes at maturation stage under soil water deficits. Colloid Surf B: Biointer 45 (2): 7-13.

Sheokand S, Bhankar V, Sawhney V, 2010. Ameliorative effect of exogenous nitric oxide on oxidative metabolism in NaCl treated chickpea plants. Braz J Plant Physiol 22 (2): 81-90. https://doi.org/10.1590/S1677-04202010000200002

Shi J, Gao L, Zuo J, Wang Q, Wang Q, Fan L, 2016. Exogenous sodium nitroprusside treatment of broccoli florets extends shelf life enhances antioxidant enzyme activity and inhibits chlorophyll-degradation. Postharvest Biol Tec 116: 98-104. https://doi.org/10.1016/j.postharvbio.2016.01.007

Silva EN, Ferreira-Silva SL, Fontenele AV, Ribeiro RV, Viégas RA, Silveira JAG, 2010. Photosynthetic changes and protective mechanisms against oxidative damage subjected to isolated and combined drought and heat stresses in Jatropha curcas plants. J Plant Physiol 167: 1157-1164. https://doi.org/10.1016/j.jplph.2010.03.005

Simontacchi M, Buet A, Lamattina L, Puntarulo S, 2012. Exposure to nitric oxide increases the nitrosyl-iron complexes content in sorghum embryonic axes. Plant Sci 183: 159-166. https://doi.org/10.1016/j.plantsci.2011.08.006

Tariq A, Masroor M, Khan A, Jaime A, da Teixeira S, Mohd I, Naeem M 2011. Role of salicylic acid in promoting salt stress tolerance and enhanced artemisinin production in Artemisia annua L. J Plant Growth Regul 30: 425-435. https://doi.org/10.1007/s00344-011-9205-0

Watanabe K, Tanaka T, Kuramochi H, Takeuchi Y, 2000. Improving salt tolerance of cotton seedling with 5-aminolevulinic acid. Plant Growth Regul 32: 97-101. https://doi.org/10.1023/A:1006369404273

Yildirim E, Donmez MF, Turan M, 2008. Use of bioinoculants in ameliorative effect on radish (Raphanus sativus L.) plants under salinity stress. J Plant Nutr 31: 2059-2074. https://doi.org/10.1080/01904160802446150

Zhang S, Chen F, Peng S, Ma W, Korpelainen H, Li C, 2010. Comparative physiological, ultrastructural and proteomic analyses reveal sexual differences in the responses of Populus cathayana under drought stress. Proteomics 10: 2661-2677. https://doi.org/10.1002/pmic.200900650

Zhu SH, Sun LN, Liu MC, Zhou J, 2008. Effect of nitric oxide on reactive oxygen species and antioxidant enzymes in kiwifruit during storage. J Sci Food Agric 88: 2324-2331. https://doi.org/10.1002/jsfa.3353

Published
2018-12-19
How to Cite
Farouk, S., & Arafa, S. A. (2018). Mitigation of salinity stress in canola plants by sodium nitroprusside application. Spanish Journal of Agricultural Research, 16(3), e0802. https://doi.org/10.5424/sjar/2018163-13252
Section
Plant physiology