Short communication. Phylogeny and genetic diversity within Iberian populations of Ornithopus L. and Biserrula L. estimated using ITS DNA sequences T
Abstract
Genetic diversity within Iberian populations of Ornithopus pinnatus, O. compressus, O. sativus and Biserrula pelecinus were assessed using ITS1 and ITS2 DNA sequences from sixty four specimens, and a phylogeny between Ornithopus species was estimated. Generally within-species variation was low, particularly within Ornithopus. The Mediterranean species of Ornithopus form a sister clade relative to the South American O. micranthopus. The sometimes considered a full species, O. sativus isthmocarpus, was not distinct from O. sativus. Between some species there is limited genetic divergence using these markers, although the situation of O. perpusillus requires additional specimens to be examined before firm conclusions can be drawn.
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References
Allan GJ, Porter JM, 2000. Tribal delimitation and phylogenetic relationships of Loteae and Coronilleae (Faboideae: Fabaceae) with special reference to Lotus: evidence from nuclear ribosomal ITS sequences. Am J Bot 87: 1871-1881. http://dx.doi.org/10.2307/2656839 PMid:11118424
Chen SL, Yao H, Han JP, Liu C, Song JY, Shi LC, Zhu YJ, Ma XY, Gao T, Pang XH et al., 2010. Validation of the ITS 2 region as a novel DNA barcode for identifying medicinal plant species. PLOS One 5: e8613. http://dx.doi.org/10.1371/journal.pone.0008613 PMid:20062805 PMCid:2799520
Clement M, Posada D, Crandall KA, 2000. TCS: a computer program to estimate gene genealogies. Mol Ecol 9: 1657-1660. http://dx.doi.org/10.1046/j.1365-294x.2000.01020.x PMid:11050560
Del Pozo A, Ovalle C, 2009 Productivity and persistence of yellow serradela (Ornithopus compressus L.) and Biserrula (Biserrula pelecinus L.) in the Mediterranean climate region of central Chile. Chilean J Agr Res 69: 340-349.
Doole GJ, Pannell DJ, Revell CK, 2009. Economic contribution of French serradella (Ornithopus sativus Brot.) pasture to integrated weed management in Western Australian mixed-farming systems: an application of compressed annealing. Aust J Agr Res Econ 53: 193-212. http://dx.doi.org/10.1111/j.1467-8489.2007.00448.x
Felsenstein J, 1985. Confidence and phylogenies: an approach using the bootstrap. Evolution 39: 783-791. http://dx.doi.org/10.2307/2408678
Fu SM, Hampton JG, Williams WM, 1994. Description and evaluation of serradella (Ornithopus L.) accessions. NZ J Agr Res 37: 471-479. http://dx.doi.org/10.1080/00288233.1994.9513086
Hall TA, 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Series 41: 95-98.
Huelsenbeck JP, Ronquist F, 2001. MRBAYES: Bayesian inference of phylogeny. Bioinformatics 17: 754-755. http://dx.doi.org/10.1093/bioinformatics/17.8.754 PMid:11524383
Loi A, Cocks PS, Howieson JG, Carr SJ, 1997. Morphological characterization of Mediterranean populations of Biserrula pelecinus L. Plant Breeding 116: 171-176. http://dx.doi.org/10.1111/j.1439-0523.1997.tb02173.x
Loi A, Howieson JG, Crocks PS, Carr SJ, 1999. Genetic variation in populations of two Mediterranean annual pasture legumes (Biserrula pelecinus L. and Ornithopus compressus L.) and associated rhizobia. Aust J Agr Res 50: 303-313. http://dx.doi.org/10.1071/A98058
Nichols P, Loi A, Nutt B, Snowball R, Revell C, 2010. Domestication of new Mediterranean annual pasture legumes. In: Sustainable use of genetic diversity in forage and turf breeding (Huyghe C, ed.). Springer Sci, pp: 137-141. http://dx.doi.org/10.1007/978-90-481-8706-5_19
Posada D, Crandall KA, 1998. Modeltest: Testing the model of DNA substitution. Bioinformatics 14: 817-818. http://dx.doi.org/10.1093/bioinformatics/14.9.817 PMid:9918953
Swofford D, 2002. PAUP* vers. 4.0b10. Phylogenetic analysis using parsimony and other methods. Sinauer Assoc, Sunderland, MA, USA.
Vicente CSL, Perez-Fernandez MA, Pereira G, Tavares De Sousa MM, 2009. Biodiversity of root-nodule bacteria associated with the leguminous plant Biserrula pelecinus. Soil Sci 174: 424-429. http://dx.doi.org/10.1097/SS.0b013e3181b655a2
Visnevschi-Necrasov T, Harris DJ, Faria MA, Pereira G, Nunes E, 2011. Genetic diversity within Scorpiurus species from the Iberian Peninsula estimated using ITS DNA sequences. Span J Agric Res 9: 198-201.
Wang XD, Wang ZP, Zou YP, 1996. An improved procedure for the isolation of nuclear DNA from leaves of wild grapevine dried with silica gel. Plant Mol Biol Rep 14: 369-373. http://dx.doi.org/10.1007/BF02673369
White TJ, Bruns TD, Lee SB, Taylor JW, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR–Protocols and applications–A laboratory manual. Academic Press, NY.
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