Conservation of potato (Solanum tuberosum L.) cultivars by cryopreservation

  • L. Barandalla NEIKER, Instituto Vasco de Investigación y Desarrollo Agrario. Vitoria
  • I. Sánchez Neiker-Insto. Vasco Inv y Desarrollo Agrario. Vitoria
  • E. Ritter Centro de Conservación de la Biodiversidad Agrícola de Tenerife. Taroconte (Tenerife)
  • J.I. Ruiz de Galarreta NEIKER, Instituto Vasco de Investigación y Desarrollo Agrario. Vitoria
DOI: https://doi.org/10.5424/sjar/2003014-43
Keywords: SOLANUM TUBEROSUM, APICAL MERISTEMS, FREEZING, BIOLOGICAL PRESERVATION, IN VITRO REGENERATION, GERMPLASM CONSERVATION

Abstract

The shoot-tips of plants of 10 potato cultivars maintained by micropropagation were frozen (ultra rapid freezing) and cryopreserved in liquid nitrogen for three months. The survival rate of the thawed specimens exceeded 50%, and even reached 100% in the case of the cultivar Fénix. The average regeneration rate was between 2.5% and 22.0%, depending on the cultivar and the presence of extra plant hormones in the regeneration medium. Cytogenetic stability was confirmed by flow cytometry: no polyploid plants were found. The regenerated plants showed vegetative development characteristics of their respective cultivars.

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Published
2003-12-01
How to Cite
Barandalla, L., Sánchez, I., Ritter, E., & Ruiz de Galarreta, J. (2003). Conservation of potato (Solanum tuberosum L.) cultivars by cryopreservation. Spanish Journal of Agricultural Research, 1(4), 9-13. https://doi.org/10.5424/sjar/2003014-43
Issue
Vol. 1 No. 4 (2003)
Section
Invisible

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