Short communication. PCR detection of DNA of bovine, ovine-caprine and porcine origin in feed as part of a bovine spongiform encephalopathy control program
Keywords:
bone meal, BSE, industrial samples, polymerase chain reaction
Abstract
The rapid identification of residues of mammalian materials in animal feedstuffs is important for the effective control of feed as a potential source of transmission of bovine spongiform encephalopathy (BSE). It is included in the monitoring program developed to avoid the appearance of this disease. In the present work a PCR analysis was tested for the detection of mammalian residues (bovine, caprine, ovine and porcine). The DNA extraction method utilised guanidium thiocianate, and primers flanking a conserved region of mitochondrial DNA were synthesized for each species. PCR tests were specific for each species and allowed detection in feedstuffs of levels as low as 1% of bovine DNA and 1.5% of ovine, caprine and porcine DNA. These PCR tests may allow the rapid detection of residues of these species constituting a powerful tool against BSEDownloads
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Published
2013-11-21
How to Cite
Corona, B., Lleonard, R., Carpio, Y., Uffo, O., & Martínez, S. (2013). Short communication. PCR detection of DNA of bovine, ovine-caprine and porcine origin in feed as part of a bovine spongiform encephalopathy control program. Spanish Journal of Agricultural Research, 5(3), 312-317. https://doi.org/10.5424/sjar/2007053-5342
Issue
Section
Animal Health
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