Enhancing germination and seedling development in lettuce and tomato using sustainable organic substrates
Abstract
Aim of study: The aim of this study was to evaluate the effects of organic substrates formulated with different proportions of acerola (Malpighia glabra) bagasse and carnauba palm (Copernica prunifera) bagana on the initial development of tomato and lettuce seedlings, with a focus on enhancing the sustainability of horticultural practices.
Area of study: This research was conducted in a controlled greenhouse environment at the Center for Agricultural Sciences, Federal University of Piauí, in Teresina, Piauí, Brazil.
Material and methods: The experiment involved 12 different substrates, each formulated from an organic compost mixed with acerola bagasse and carnauba palm bagana. Basaplant®, a commercially available substrate, was used as the control. This study evaluated the following variables: seed emergence (SE), emergence speed index (ESI), total dry mass (TDM), shoot dry mass (SDM), root volume (RV), dry root mass (DRM), seedling height (SH), leaf area (LA), stem diameter (SD), fresh root mass (FRM), fresh shoot mass (FSM), and Dickson quality index (DQI). The statistical analysis included principal component analysis (PCA) and cluster analysis to determine the effectiveness of the substrates.
Main results: For tomato, substrates with 50% carnauba bagana displayed higher volumetric density, which positively correlated with RV and DRM. This benefited seedling stability and water retention. Fine particles (<0.25 mm) enhanced TDM, while particles between 0.5–0.25 mm negatively affected SE. In lettuce, substrates with 50% acerola bagasse promoted higher aeration and drainage, which was beneficial for this crop. Larger particles (2.0–1.0 mm) correlated positively with LA, while finer particles reduced root dry mass. PCA and clustering grouped substrates by growth performance, with the G3 cluster favoring tomato and the G5 cluster favoring lettuce.
Research highlights: This study demonstrates that organic substrates with 50% carnauba bagana improve root growth and water retention in tomato seedlings, while substrates with 50% acerola bagasse enhance aeration and drainage, thereby bene- fiting lettuce growth. Fine particles positively influence biomass accumulation in tomato but limit root development in lettuce. Conversely, larger particles support greater leaf area in lettuce while reducing root depth. PCA revealed distinct substrate pref- erences for tomato and lettuce, underscoring the importance of tailoring substrate composition to crop-specific needs.
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References
Albano FG, Cavalcante IHL, Machado JS, Lacerda CF, Silva ER, Sousa HG, 2017. New substrate containing agroindustrial carnauba residue for production of papaya under foliar fertilization. Rev. Bras. Eng. Agríc. Ambient. 21(2):128–133. https://doi.org/10.1590/1807-1929/agriambi.v21n2p128-133
Alvares CA, Staple JL, Sentelhas PC, Gonçalves JLM, Sparovek G, 2013. Köppen’s Climate Classification Map for Brazil. Meteorol. Z. 22:711–728. https://doi.org/10.1127/0941-2948/2013/0507
Arnhold E, 2013. Package in the R environment for analysis of variance and complementary analyses. Braz. J. Vet. Res. Anim. Sci. 50:488–492. https://doi.org/10.11606/issn.1678-4456.v50i6p488-492
Basso SMSC, 1999. Caracterização morfológica e fixação biológica de nitrogênio de espécies de Adesmia DC. e Lotus L. Tese de Doutorado. Universidade Federal do Rio Grande do Sul, Porto Alegre. 268 pp.
Bernal MP, Alburquerque JA, Moral R, 2009. Composting of animal manures and chemical criteria for compost maturity assessment: A review. Bioresour. Technol. 100:5444–5453. https://doi.org/10.1016/j.biortech.2008.11.027
BRASIL. Ministério da Agricultura, Pecuária e Abastecimento, 2007. Instrução Normativa nº 17, de 7 de abril de 2017. Brasília, DF: MAPA. https://www.gov.br/agricultura/pt-br/assuntos/insumos-agropecuarios/insumos-agricolas/fertilizantes/legislacao/in-17-de-21-05-2007-aprova-metodo-substrato.pdf
Castro VC, Fernandes MM, Fernandes MRM, Nóbrega RSA, 2016. Avaliação de diferentes substratos orgânicos para mudas de Enterolobium contortisiliquum em uma área desertificada. Rev. Agrogeoambiental 8(3):101–109. https://doi.org/10.18406/2316-1817v8n32016884
Funes-Pinter I, Salomón MV, Martín JN, Uliarte EM, Hidalgo A, 2022. Effect of bioslurries on tomato (Solanum lycopersicum L.) and lettuce (Lactuca sativa) development. Rev. Fac. Cienc. Agrar. 54:48–60. https://doi.org/10.48162/rev.39.082
Giuffrida F, Consoli S, 2016. Reusing perlite substrates in soilless cultivation: analysis of particle size, hydraulic properties, and solarization effects. J. Irrig. Drain. Eng. 142:04015047. https://doi.org/10.1061/(ASCE)IR.1943-4774.0000968
Godfray HCJ, Beddington JR, Crute IR, Haddad L, Lawrence D, Muir JF, Pretty J, Robinson S, Thomas SM, Toulmin C, 2010. Food security: the challenge of feeding 9 billion people. Science 327:812–818. https://doi.org/10.1126/science.1185383
Griebeler AM, Araujo MM, Turchetto F, Berghetti ÁLP, Rorato DG, Fermino MH, Aimi SC, Costella C, 2023. Organic waste and controlled-release fertilizer maximize the growth of Citharexylum montevidense in nursery and in the field. CERNE 29:e103003. https://doi.org/10.1590/01047760202329013003
Gutiérrez-Castorena MDC, Escobar JH, Ortiz-Solorio CA, Sánchez RA, Lara EH, 2011. Relación porosidad-retención de humedad en mezclas de sustratos y su efecto sobre variables respuesta en plántulas de lechuga. Rev. Chapingo Ser. Horticultura 17:183–196.
Karsinah, Prajanti SDW, Amelia DR, 2023. Organic mulch innovation from straw waste in the context of realizing climate smart agriculture as an acceleration of green economy achievement. J. Law Sustain. Dev. 11:e2281. https://doi.org/10.55908/sdgs.v11i12.2281
Kassambara A, Mundt F, 2020. factoextra: extract and visualize the results of multivariate data analyses. R package version 1.0.7.
Kassambara A, 2020. ggpubr: ‘ggplot2’ based publication ready plots. R package version 0.4.0.
Kiehl EJ, 2004. Manual de Compostagem: maturação e qualidade do composto. 4ª ed. Piracicaba: E. J. Kiehl.
Leite MRL, Costa RM, Matos SS, Andrade HAF, Silva-Matos RRS, 2023. Production of pre-sprouted sugarcane seedlings using carnauba bagana as substrate. Pesqui. Agropecu. Trop. 53:e74082. https://doi.org/10.1590/1983-40632023v5374082
Maechler M, Rousseeuw P, Struyf A, Hubert M, Hornik K, 2021. Cluster: cluster analysis basics and extensions. R package version 2.1.2.
Mahmoud AMA, Mohamed Y, El-Helaly MA, Afifi MMI, El-Tawashy MKF, 2024. Potential of producing organic lettuce seedlings without peat using agricultural and agro-industrial compost. J. Plant Nutr. 47(19):3316–3342. https://doi.org/10.1080/01904167.2024.2378927
Maguire JD, 1962. Speed of germination—aid in selection and evaluation for seedling emergence and vigor. Crop Sci. 2:176–177. https://doi.org/10.2135/cropsci1962.0011183X000200020033x
Mendonça AM, Natale W, Sousa GG, Silva Junior FB, 2021. Morphophysiology and nutrition of yellow passion fruit seedlings grown in substrates based on carnauba palm bagana. Rev. Bras. Cien. Agra. 16(3):e132. https://doi.org/10.5039/agraria.v16i3a132
MirseyedHosseini H, Alavipoor E, Delshad M, 2023. Evaluation of different growth media for tomato seedlings to optimize production and water use. Iran Agric. Res. 36(2):61–70. https://doi.org/10.22099/iar.2017.4138
Mo J, Yang Q, Zhang N, Zhang W, Zheng Y, Zhang Z, 2018. A review on agro-industrial waste (AIW) derived adsorbents for water and wastewater treatment. J. Environ. Manage. 227:395–405. https://doi.org/10.1016/j.jenvman.2018.08.069
Monteiro AS, Barbosa MM, Silva FFM, Bezerra RF, Maia KS, 2020. Preparation, phytochemical and bromatological evaluation of flour obtained from the acerola (Malpighia punicifolia) agroindustrial residue with potential use as fiber source. LWT 134:110142. https://doi.org/10.1016/j.lwt.2020.110142
Olaria M, Nebot JF, Molina H, Troncho P, Lapeña L, Llorens E, 2016. Effect of different substrates for organic agriculture in seedling development of traditional species of Solanaceae. Span. J. Agric. Res. 14:e0801. https://doi.org/10.5424/sjar/2016141-8013
Parada F, Ercilla-Montserrat M, Arcas-Pilz V, Lopez-Capel E, Carazo N, Montero JI, Gabarrell X, Villalba G, Rieradevall J, Pere Muñoz, 2021. Comparison of organic substrates in urban rooftop agriculture, toward improving crop production resilience to temporary drought in Mediterranean cities. J. Sci. Food Agric. 101:5888–5897. https://doi.org/10.1002/jsfa.11241
R Core Team, 2022. R: A language and environment for statistical computing. Vienna: R Foundation for Statistical Computing.
Rasband WS, 2004. ImageJ. National Institutes of Health, Bethesda, Maryland, USA. http://rsb.info.nih.gov/ij/
Ripp PG, Gusmão AP, Lorin HEF, Costa MSS de M, Edwiges T, 2020. Composting process in the production of lettuce seedling substrates: effect of covering and turning frequency. Eng. Agric. 40:562–570. https://doi.org/10.1590/1809-4430-eng.agric.v40n5p562-570/2020
Silva NC da, Dourado PLA, Tosi MM, 2023. Uma revisão sobre os subprodutos da acerola (Malpighia emarginata DC.) como uma matéria-prima promissora para a geração de produtos verdes. Rev. Bras. Tecnol. Alimentar 26:e2023039. https://doi.org/10.1590/1981-6723.03923
Silva TV, Melo HC, Campos LFC, Tarazi MFA, Cunha Junior LC, Nascimento AR, 2021. Growth and productivity of tomato under use of biostimulant and ages of seedling transplantation. Colloq. Agrariae 17:23–33. https://doi.org/10.5747/ca.2021.v17.n4.a446
Sousa MO, Matos RRSS, Cardoso JPS, Cordeiro KV, Barbosa LMP, Santos JF, Oliveira ARF, Machado FGA, Costa NA, Oliveira MMT, 2020. Bagana de carnaúba como substrato na produção de mudas de açaí cultivar BRS-Pará. Rev. Ibero-Am. Cien. Amb. 11(5):113–122. https://doi.org/10.6008/CBPC2179-6858.2020.005.0012
Zuffo AM, González-Aguilera J, Lima RE, Zaratín Alves C, 2020. Substrates for the production of lettuce seedlings. Eur. J. Hortic. Sci. 85:372–379. https://doi.org/10.17660/eJHS.2020/85.5.9
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