Performance, carcass yield, and intestinal morphology of broilers supplemented with Saccharomyces boulardii or Saccharomyces cerevisiae and reared on different litter substrates
Abstract
Aim of study: To evaluate the performance, carcass yield, and intestinal morphology of broilers supplemented with the yeasts Saccharomyces boulardii or Saccharomyces cerevisiae and housed on wood shavings, rice husk, or a 50% mixture of these substrates.
Area of study: South Brazil.
Material and methods: A total of 270 one-day-old Cobb 500 broilers were housed, in a 3x3 factorial scheme within a completely randomized design, distributed in 45 experimental pens (experimental units) resulting in 9 treatments with 5 replications each. Feed consumption, weight gain, and feed conversion of birds were evaluated in the period from 1 to 21 days of age, 22 to 42 days, and over the entire experimental period. Additionally, the weight of cuts and viscera was assessed after euthanasia at 42 days of age. A part of the ileum of approximately one centimeter was sampled to evaluate the height of the villi, depth of the crypts, and villus:crypt ratio.
Main results: No significant interactions were found on performance variables. Birds raised on rice husk showed higher weight gain and longer ileum villi compared to other litters. Yeast supplementation influenced the weight of cuts and viscera, with notable interactions between substrate types, crypt length and villus ratios remained unaffected.
Research highlights: These findings emphasize the importance of considering rice husk as an effective alternative litter, as it improved body weight gain and intestinal villus length in broilers. Additionally, dietary yeast supplementation influenced cut and viscera weight interactions, with significant effects observed in different substrate combinations.
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References
Abudabos A, Alyemni A, Al Marshad BA, 2016. Bacillus subtilis PB6 based-probiotic (CloSTATTM) improves intestinal morphological and microbiological status of broiler chickens under Clostridium perfringens challenge. Int J Agric Biol. 15: 978–982.
Ahn GC, Jang SS, Lee KY, Kwak WS, Oh YK, Park KK, 2016. Characteristics of sawdust and cocopeat beddings, and their usefulness according to the fan and pen location for rearing Hanwoo cattle. Asian-Australas J Anim Sci 29(3): e444. https://doi.org/10.5713/ajas.15.0672
Alagawany M, Abd El-Hack ME, Farag MR, Sachan S, Karthik K, Dhama K, 2018. The use of probiotics as ecofriendly alternatives for antibiotics in poultry nutrition. Environ Sci Pollut Res 25(11): 10611–10618. https://doi.org/10.1007/s11356-018-1687-x
Anadón A, Martínez-Larrañaga MR, Martínez MA, 2006. Probiotics for animal nutrition in the European Union. Regulation and safety assessment. Regul Toxicol Pharm 45(1): 91–95. https://doi.org/10.1016/j.yrtph.2006.02.004
Barros BOJ, Ramos RB, Corredor MJR, Pulecio SSL, Gómez GD, Ochoa AJ, 2021. Association between productive parameters and intestinal histomorphological findings in broilers supplemented with probiotics (Saccharomyces cerevisiae, Lactobacillus acidophilus and Bacillus subtilis). Int J Morphol 39(5): 1493–1501. https://doi.org/10.4067/S0717-95022021000501493
Beçak W, Paulete J, 1976. Técnicas de citologia e histologia. Livros Técnicos e Científicos Editora SA, Rio de Janeiro. 230 pp.
Bilal RM, Hassan FU, Saeed M, Rafeeq M, Zahra N, Fraz A, Saeed S, Khan MA, Mahgoub HA, Farag MR, Alagawan M, 2023. Role of yeast and yeast-derived products as feed additives in broiler nutrition. Anim Biotechnol 34(2): 392-401. https://doi.org/10.1080/10495398.2021.1942028
Ding B, Zheng J, Wang X, Zhang L, Sun D, Xing Q, Pirone A, Fronte B, 2019. Effects of dietary yeast beta-1,3-1,6-glucan on growth performance, intestinal morphology and immunity parameters in Haidong chicks. Asian-Australas J Anim Sci 32(10): 1558–1564. https://doi.org/10.5713/ajas.18.0962
Forte C, Manuali E, Abbate Y, Papa P, Vieceli L, Tentellini M, Trabalza-Marinucci M, Moscati L, 2018. Dietary Lactobacillus acidophilus positively influences growth performance, gut morphology, and microbiology in rurally reared chickens. Poultr Sci 97(1): 930–936. https://doi.org/10.3382/ps/pex396
Furlan RL, Macari M, Luquetti BC, 2004. Como avaliar os efeitos do uso de prebióticos, probióticos e flora de exclusão competitiva. Simpósio técnico de incubação, matrizes de corte e nutrição, Balneário Camboriú (Santa Catarina), Oct 04–06. pp. 6–28.
Garcês APJT, Afonso SMS, Chilundo A, Jairoce CTS, 2013. Evaluation of different litter materials for broiler production in a hot and humid environment: 1. Litter characteristics and quality. J Appl Poult Res 22(2): 168–176. https://doi.org/10.1007/s11250-016-1202-7
Gao J, Zhang HJ, Yu SH, Wu SG, Yoon I, Quigley J, Gao YP, Qi GH, 2008. Effects of yeast culture in broiler diets on performance and immunomodulatory functions. Poultr Sci 87(7): 1377–1384. https://doi.org/10.3382/ps.2007-00418
Gava MS, 2012. Metodologia de morfometria intestinal em frangos de corte. Master’s thesis. Univ. Fed. do Rio Grande do Sul, Brasil. 61 pp.
Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, Morelli L, Canani RB, Flint HJ, Salminen S, et al., 2014. Expert consensus document: The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol 11(8): 506–514. https://doi.org/10.1038/nrgastro.2014.66
Ibrahim ZA, 2011. Modulation of immunity and biological functions of japanese quail by mannan oligosaccharide and β-glucan administration. Egypt Poult Sci J 31(4): 867–882.
Jensen GS, Patterson KM, Yoon I, 2008. Yeast culture has anti-inflammatory effects and specifically activates NK cells. Comp Immunol Microbiol Infect Dis 31(6): 487–500. https://doi.org/10.1016/j.cimid.2007.08.005
Jorge MPB, Vaz RGMV, Da Silva MC, De Araujo Costa H, 2020. Desempenho de frangos de corte criados sobre dois tipos de cama e duas densidades. Desafios Rev Interdiscip Fed Tocantins 7(especial): 221–228. https://doi.org/10.20873/uftsuple2020-8624
Kim YS, Kim BT, Lee CH, 2007. Variations of physical properties depending on the height of reactor in vertical composting process. J Korea Org Resour Recycl Assoc 15(4): 115–124.
Krysiak K, Konkol D, Korczyński M, 2021. Overview of the use of probiotics in poultry production. Animals 11(6): e1620. https://doi.org/10.3390/ani11061620
Lima DKS, Pessoa MS, Arhnold E, Leite PRSC, Leonídio ARA, Santos RLR, Eickstein C, Pinto VM, Abrão FO, 2020. Intestinal and immunological histological parameters of broilers supplemented with commercial probiotic or fungi of the autochthonous microbiota. Braz J Vet Med 42: e101220. https://doi.org/10.29374/2527-2179.bjvm101220
Liu N, Wang JQ, Jia SC, Chen YK, Wang JP, 2018. Effect of yeast cell wall on growth performance and gut health of broilers challenged with aflatoxin B1 and necrotic enteritis. Poultr Sci 97(2): 477–484. https://doi.org/10.3382/ps/pex342
Macari M, Furlan RL, 2001. Ambiência na produção de aves em clima tropical. In: SILVA IJO (Ed.). Piracicaba: FUNEP. pp. 31–87.
Marchini CFP, Silva PL, Nascimento MRBM, Beletti ME, Guimarães EC, Soares HL, 2009. Morfometria da mucosa duodenal em frangos de corte submetidos à temperatura ambiente cíclica elevada. Arq Bras Med Vet Zootec 62(2): 491–497. https://doi.org/10.1590/S0102-09352009000200029
Markovic R, Sefer D, Krstic M, Petrujkic B, 2009. Effect of different growth promoters on broiler performance and gut morphology. Arch Med Vet 41(2): 163–169. https://doi.org/10.4067/S0301-732X2009000200010
Mountzouris KC, Tsirtsikos P, Kalamara E, Nitsch S, Schatzmayr G, Fegeros K, 2007. Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in promoting broiler performance and modulating cecal microflora. Poultr Sci 86(2): 309–317. https://doi.org/10.1093/ps/86.2.309
Munir MT, Belloncle C, Irle M, Federighi M, 2019. Wood-based litter in poultry production: a review. World’s Poult Sci J 75(1): 5–16. https://doi.org/10.1017/S0043933918000909?urlappend=%3Futm_source%3Dresearchgate
Quevedo DM, Ochoa JE, Corredor JR, Pulecio SL, 2020. Efectos de la adición de probiótico Saccharomyces cerevisiae sobre histomorfología intestinal en pollos de engorde. Rev Med Vet Zoot 67(3): 239–252. https://doi.org/10.1446/rfmvz.v67n3.93931
Rajput IR, Li LY, Xin X, Wu BB, Juan ZL, Cui ZW, Li WF, 2013. Effect of Saccharomyces boulardii and Bacillus subtilis B10 on intestinal ultrastructure and mucosal immunity in broilers. Poultr Sci 92(4): 956–965. https://doi.org/10.3382/ps.2012-02845
Ramadan SGA, Mahboub HDH, Helal MA, Gaafar KM, 2013. Behaviour, welfare and performance of broiler chicks reared on different litter materials. Assiut Vet Med J 59(138): 9–18. https://doi.org/10.21608/avmj.2013.171599
R Core Team (2022). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://CRAN.R-project.org/package=MultivariateAnalysis
Ribeiro CAO, Grötzner SR, Filho HSR, 2012. Técnicas e Métodos para Utilização Prática em Microscopia. Editora Santos. 420 pp.
Roever L, 2016. Compreendendo os estudos de metanálise na pesquisa clínica. Rev Soc Bras Clin Med 14(4): 245–249.
Rosário M, Silva M, Coelho A, Savino V, Dias C, 2008. Canonical discriminant analysis applied to broiler chicken performance. Animal 2(3): 419–424. https://doi.org/10.1017/s1751731107001012
Santos U, Ramos CO, Sanches NM, Sousa FF, 2010. Propriedade antibacteriana dos frutos de Sucupira-Branca (Pterodon pubescens). Rev Eletrônica Biol 3(4): 77–88.
Shareef AM, Al-Dabbagh ASA, 2009. Effect of probiotic (Saccharomyces cerevisiae) on performance of broiler chicks. Iraqi J Vet Sci 23(1): 23–29. https://doi.org/10.33899/ijvs.2009.189533
Shen YB, Piao XS, Kim SW, Liu P, Yoon I, Zhen YG, 2009. Effects of yeast culture on growth performance, intestinal health, and immune response of nursery pigs. J Anim Sci 87(8): 2614–2624. https://doi.org/10.2527/jas.2008-1512
Sousa RF, Dourado LRB, Lopes JB, Fernandes ML, Kato RK, Nascimento DEN, Sakomura NK, Lima SBP, Ferreira GJBC, 2019. Effect of an enzymatic blend and yeast on performance, carcass yield and histomorphometry of broilers from 21 to 42 days. Braz J Poult Sci 21(2): 1–6. https://doi.org/10.1590/1806-9061-2018-0758
Toledo TDSD, Pich CS, Roll AAP, Dai Prá MA, Leivas Leite F, Goncalves Xavier E, Roll VFB, 2019. The effect of litter materials on broiler performance: a systematic review and meta-analysis. Braz J Poult Sci 60(6): 605–616. https://doi.org/10.1080/00071668.2019.1639143
Von Mühlen C, Roll AP, Dias RC, Freitas BK, Xavier EG, Roll VF, 2021. Características físicas e absortivas da casca de arroz e da maravalha usadas como cama avícola. XXX CIC-Congresso de Iniciação Científica, UFPel, Pelotas (Brasil), Oct 18–22.
Zarei A, Lavvaf A, Motlagh MM, 2017. Effects of probiotic and whey powder supplementation on growth performance, microflora, and ileum morphology in broilers. J Appl Anim Res 46(1): 840–844. https://doi.org/10.1080/09712119.2017.1410482
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