Unraveling the morpho-pathogenic and molecular variability of Exserohilum turcicum causing turcicum leaf blight in maize (Zea mays L.)
Abstract
Aim of study: This study investigated the prevalence, morphological, molecular and haplotype diversity and virulence of Exserohilum turcicum isolates causing turcicum leaf blight disease in maize.
Area of study: Nine maize-growing districts of West Bengal, India.
Material and methods: A roving survey were conducted to assess the prevalence of disease (turcicum leaf blight) and to collect samples. Cultural and morphological characterization was performed on Potato Dextrose Agar medium. Molecular characterization involved the isolation of genomic DNA, followed by amplification of the ITS 1 and 4 regions and sequencing. A virulence study was carried out under controlled conditions in a greenhouse.
Main results: Roving surveys during the Rabi season 2020-2021 revealed varying disease severity across maize-growing regions, Cultural and morphological characterization of fifteen isolates exhibited significant diversity in colony characteristics and sporulation patterns. Molecular characterization and phylogenetic analysis resulted in isolates clustering geographically and high genetic diversity with each isolate representing a unique haplotype. Virulence assessments highlighted variability in virulence index among isolates, suggesting diverse virulence profiles.
Research highlights: This research significantly advances our understanding of disease severity, genetic diversity and pathogenicity mechanisms, furnishing valuable insights to aid in further research to understand the genetic factors contributing to virulence variation and develop effective disease control measures.
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References
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