Assessing boar taint in Portuguese pork: A small-scale study of prevalence and classification via established detection thresholds
Abstract
Aim of study: To assess the boar taint prevalence in the Portuguese pork industry through an observational study model by measuring skatole and androstenone levels in fat and to compare it with the estimated thresholds for these compounds among Portuguese consumers.
Area of study: Portugal.
Material and methods: Adipose tissue samples from 140 animals (102 entire males and 38 females) were collected in three Portuguese abattoirs for boar taint quantification. Cut-off limits were determined using best-estimate thresholds (BET) for skatole and androstenone.
Main results: Boar taint quantification for the 140 animals revealed levels of 36.0±4.7 ng/g of skatole and 64.5±21.3 ng/g of androstenone, and values were significantly higher in males. Group BET values were 35.4 ng/g and 566.3 ng/g for skatole and androstenone, respectively. BET values were exceeded in 28.8% of the samples for skatole and 0.7% for androstenone.
Research highlights: The investigation revealed a generally low level of boar taint in the samples of this small-scale study on skatole and androstenone prevalence in Portugal's pork supply chain. However, occasionally a significantly elevated boar taint levels suggest that relying solely on slaughtering sexually immature males might not fully resolve this issue.
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References
Aldal I, Andresen O, Egeli AK, Haugen JE, Grodum A, Fjetland O, et al., 2005 Levels of androstenone and skatole and the occurrence of boar taint in fat from young boars. Livest Prod Sci 95(1-2): 121-129. https://doi.org/10.1016/j.livprodsci.2004.12.010
Aluwe M, Aaslyng M, Backus G, Bonneau M, Chevillon P, Haugen JE, et al., 2018. Consumer acceptance of minced meat patties from boars in four European countries. Meat Sci 137: 235-243. https://doi.org/10.1016/j.meatsci.2017.11.034
Aluwe M, Heyrman, E, Almeida JM, Babol J, Battacone G, Citek J, et al., 2020. Exploratory survey on European consumer and stakeholder attitudes towards alternatives for surgical castration of piglets. Animals 10(10): 1758. https://doi.org/10.3390/ani10101758
Annor-Frempong IE, Nute GR, Whittington FW, Wood JD, 1997. The problem of taint in pork 1. Detection thresholds and odour profiles of androstenone and skatole in a model system Meat Sci 46(1): 45-55. https://doi.org/10.1016/S0309-1740(97)00003-X
ASTM:E679-04, 2011. Standard practice for determination of odor and taste thresholds by a forced-choice ascending concentration series method of limits. ASTM International.
Baek C, Hansen-Moller J, Friis C, Cornett C, Hansen SH, 1997. Identification of selected metabolites of skatole in plasma and urine from pigs. J Agr Food Chem 45(6): 2332-2340. https://doi.org/10.1021/jf9605862
Bee G, Quiniou N, Maribo H, Zamaratskaia G, Lawlor PG, 2020. Strategies to meet nutritional requirements and reduce boar taint in meat from entire male pigs and immunocastrates. Animals 10(11): 1950. https://doi.org/10.3390/ani10111950
Bonneau M, Chevillon P, 2012. Acceptability of entire male pork with various levels of androstenone and skatole by consumers according to their sensitivity to androstenone. Meat Sci 90(2): 330-337. https://doi.org/10.1016/j.meatsci.2011.07.019
Bonneau M, Weiler U, 2019. Pros and cons of alternatives to piglet castration: welfare, boar taint, and other meat quality traits. Animals 9(11): 884. https://doi.org/10.3390/ani9110884
Borrisser-Pairo F, Panella-Riera N, Zammerini D, Olivares A, Garrido MD, Martinez B, et al., 2016. Prevalence of boar taint in commercial pigs from Spanish farms. Meat Sci 111: 177-182. https://doi.org/10.1016/j.meatsci.2015.10.001
Christensen RH, Nielsen DB, Aaslyng MD, 2019. Estimating the risk of dislike: An industry tool for setting sorting limits for boar taint compounds. Food Qual Prefer 71: 209-216. https://doi.org/10.1016/j.foodqual.2018.07.008
Claus R, Weiler U, Herzog A, 1994. Physiological-aspects of androstenone and skatole formation in the boar - a review with experimental-data. Meat Sci 38(2): 289-305. https://doi.org/10.1016/0309-1740(94)90118-X
Croghan W, Egeghy PP, 2003. Methods of dealing with values below the limit of detection using SAS Carry. Proc 2003 SouthEast SAS Users Group Conf; Sept 22-24; St. Petersburg, FL. Cary, NC: SAS Users Group, 1−4.
De Briyne N, Berg C, Blaha T, Temple D, 2016. Pig castration: will the EU manage to ban pig castration by 2018? Porc Health Manag 2: 29. https://doi.org/10.1186/s40813-016-0046-x
Doran E, Whittington FA, Wood JD, McGivan JD, 2004. Characterisation of androstenone metabolism in pig liver microsomes. Chem Biol Interact 147(2): 141-149. https://doi.org/10.1016/j.cbi.2003.12.002
EFSA, 2004. Welfare aspects of the castration of piglets. The EFSA Journal 91.
Font-i-Furnols M, Martin-Bernal R, Aluwe M, Bonneau M, Haugen JE, Moerlein D, et al., 2020. Feasibility of on/at line methods to determine boar taint and boar taint compounds: an overview. Animals 10(10): 1886. https://doi.org/10.3390/ani10101886
Fredriksen B, Johnsen AMS, Skuterud E, 2011. Consumer attitudes towards castration of piglets and alternatives to surgical castration. Res Vet Sci 90(2): 352-357. https://doi.org/10.1016/j.rvsc.2010.06.018
Haugen JE, Brunius C, Zamaratskaia G, 2012. Review of analytical methods to measure boar taint compounds in porcine adipose tissue: The need for harmonised methods. Meat Sci 90(1): 9-19. https://doi.org/10.1016/j.meatsci.2011.07.005
Klyuchnikova MA, Kvasha I, Laktionova T, Voznessenskaya VV, 2022. Olfactory perception of 5 alpha-androst-16-en-3-one: Data obtained in the residents of central Russia. Data Br 45: 108704. https://doi.org/10.1016/j.dib.2022.108704
Lundstrom K, Matthews KR, Haugen JE, 2009. Pig meat quality from entire males. Animal 3(11): 1497-1507. https://doi.org/10.1017/S1751731109990693
Panella-Riera N, Moe M, Grindflek E, Oliver MA, Wood JD, Doran O, 2008. Effect of sex steroids on expression of sulfotransferase 2B1 immunoreactive protein in primary cultured porcine hepatocytes. Livest Sci 118(3): 223-230. https://doi.org/10.1016/j.livsci.2008.01.021
Pereira-Pinto R, Barros M, Vaz-Velho M, Mata F, Pires P, 2024. Enhanced HPLC method for boar taint quantification. ChemistryOpen e202300283. https://doi.org/10.1002/open.202300283
Prusa K, Nederveld H, Runnels PL, Li R, King VL, Crane JP, 2011. Prevalence and relationships of sensory taint, 5 alpha-androstenone and skatole in fat and lean tissue from the loin (Longissimus dorsi) of barrows, gilts, sows, and boars from selected abattoirs in the United States. Meat Sci 88(1): 96-101. https://doi.org/10.1016/j.meatsci.2010.12.008
Robic A, Faraut T, Prunier A, 2014. Pathways and genes involved in steroid hormone metabolism in male pigs: A review and update. J Steroid Biochem Mol Bio 140: 44-55. https://doi.org/10.1016/j.jsbmb.2013.11.001
Sodring M, Nafstad O, Haseth TT, 2020. Change in Norwegian consumer attitudes towards piglet castration: increased emphasis on animal welfare. Acta Vet Scand 62(1): 22-22. https://doi.org/10.1186/s13028-020-00522-6
Squires EJ, Bone C, Cameron J, 2020. Pork production with entire males: directions for control of boar taint. Animals 10(9): 1665. https://doi.org/10.3390/ani10091665
Thomsen R, Edwards SA, Jensen BB, Rousing T, Sorensen JT, 2015. Effect of faecal soiling on skatole and androstenone occurrence in organic entire male pigs. Animal 9(9): 1587-1596. https://doi.org/10.1017/S1751731115000798
Van Ferneij JP, 2022. The pig castration situation in the European Union. pig333com - Professional Pigs Community Webpage. https://wwwpig333com/articles/the-pig-castration-situation-in-the-european-union_18100/ [March 11th 2022].
van Oeckel MJ, Casteels M, Warnants N, De Boever JL, van Renterghem R, Boucque CV, 1996. Production of entire males with Belgian Landrace and hybrid pigs: The incidence of sensory aberrations. J Anim Physiol Anim Nutr 76(2-3): 111-121. https://doi.org/10.1111/j.1439-0396.1996.tb00681.x
Verplanken K, Wauters J, Vercruysse V, Aluwe M, Vanhaecke L, 2017. Sensory evaluation of boar-taint-containing minced meat, dry-cured ham and dry fermented sausage by a trained expert panel and consumers Food Chem 233: 247-255. https://doi.org/10.1016/j.foodchem.2017.04.105
Walstra P, Claudi-Magnussen C, Chevillon P, Von Seth G, Diestre A, Matthews KR, et al., 1999. An international study on the importance of androstenone and skatole for boar taint: levels of androstenone and skatole by country and season. Livest Prod Sci 62(1): 15-28. https://doi.org/10.1016/S0301-6226(99)00054-8
Weiler U, Furnols MFI, Fischer K, Kemmer H, Oliver MA, Gispert M, et al., 2000. Influence of differences in sensitivity of Spanish and German consumers to perceive androstenone on the acceptance of boar meat differing in skatole and androstenone concentrations. Meat Sci 54(3): 297-304. https://doi.org/10.1016/S0309-1740(99)00106-0
Wysocki CJ, Dorries KM, Beauchamp GK, 1989. Ability to perceive androstenone can be acquired by ostensibly anosmic people. P Nat Acad Sci USA 86(20): 7976-7978. https://doi.org/10.1073/pnas.86.20.7976
Zamaratskaia G, Squires EJ, 2009. Biochemical, nutritional and genetic effects on boar taint in entire male pigs. Animal 3(11): 1508-1521. https://doi.org/10.1017/S1751731108003674
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