Variabilidad en la germinación y en la emergencia de variedades autóctonas de Medicago sativa en diferentes oasis de Túnez
Resumen
Se evaluó la germinación en laboratorio con y sin estrés salino, así como las características de las semillas y la emergencia de 20 poblaciones de la leguminosa forrajera
Medicago sativa ssp sativa, procedentes de tres localidades de Túnez. Se observaron diferencias altamente significativas entre las poblaciones (p < 0,001) en todos los caracteres estudiados excepto en el color del tegumento. El número de semillas por gramo varió entre 378 y 460. La emergencia de las semillas fue diferente entre las poblaciones y las localidades (variaron entre el 12 y el 45%). El estrés salino (150 mM) causó una fuerte reducción en todas las poblaciones, tanto en la germinación final como en el porcentaje de germinación, con una reducción media del 62,4 y 69,7%, respectivamente. Los valores de germinación finales variaron entre 26,5 y 75,75% y entre 10,75 y 32,5% con agua destilada y estrés salino, respectivamente. El porcentaje de germinación varió desde el 12,75 al 63,25% y del 6,25 al 21,25% en condición no salina y estrés salino respectivamente. El análisis cluster separó las poblaciones en cuatro grupos.
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Badger K.S., Ungar I.A., 1989. The effects of salinity and temperature on the germination of the inland halophyte Hordeum jubatum. Can J Bot 67, 1420-1425. http://dx.doi.org/10.1139/b89-188
Bayuelo-Jiménez J.S., Craig R., Lynch J.P., 2002. Salinity tolerance of Phaseolus species during germination and early seedling growth. Crop Sci 42, 1584-1594. http://dx.doi.org/10.2135/cropsci2002.1584
Benabderrahim M.A., Haddad M., Ferchichi A., 2009. Diversity of lucerne (Medicago sativa L.) populations in south Tunisia. Pak J Bot 41(6), 2851-2861.
Berdahl J.D., Mayland H.F., Asay K.H., Jefferson P.G., 1999. Variation in agronomic and morphological traits among Russian wildrye accessions. Crop Sci 39, 1890-1895. http://dx.doi.org/10.2135/cropsci1999.3961890x
Bewley J.D., 1997. Seed germination and dormancy. Plant Cell 9, 1055-1066. http://dx.doi.org/10.1105/tpc.9.7.1055 PMid:12237375 PMCid:156979
Bolaños-Aguilar E.D., Huyghe C., Julier B., Ecalle, C., 2000. Genetic variation for seed yield and its components in alfalfa (Medicago sativa L.) populations. Agronomie 20, 333-345. http://dx.doi.org/10.1051/agro:2000131
Bregard A., Belanger G., Michaud R., Tremblay G.F., 2001. Biomass partitioning, forage nutritive value and yield of contrasting genotypes of timothy. Crop Sci 41, 1212-1219. http://dx.doi.org/10.2135/cropsci2001.4141212x
Bruulsema T.W., Christie B.R., 1987. Nitrogen contribution to succeeding corn from alfalfa and red clover. Agron J 79, 96-100. http://dx.doi.org/10.2134/agronj1987.00021962007900010020x
Campbell C.A., Zentner R.P., Janzen H.H., Bowren K.E., 1990. Crop rotation studies on the Canadian prairies. Publication 1841/E, Canadian Government Publication Center, Ottawa, Canada. 110 pp.
Crochemore M.L., Huyghe C., Kerlan M.C., Durand F., Julier B., 1996. Partitioning and distribution of RAPD variation in the Medicago sativa complex, Agronomie 16, 421-432. http://dx.doi.org/10.1051/agro:19960702
Crochemore M.L., Huyghe C., Ecalle C., Julier B., 1998. Structuration of alfalfa genetic diversity using agronomic and morphological characteristics, relationships with RAPD markes. Agronomie 18, 79-94. http://dx.doi.org/10.1051/agro:19980106
Diwan N., Bouton J.H., Kochert G., Cregan P.B., 2000. Mapping of simple sequence repeat (SSR) DNA markers in diploid and tetraploid alfalfa. Theor Appl Genet 101, 165-172. http://dx.doi.org/10.1007/s001220051465
El-Keblawy A., Al-Rawai A., 2005. Effects of salinity, temperature and light on germination of invasive Prosopis juliflora (Sw) DC J Arid Environ 6, 555-565. http://dx.doi.org/10.1016/j.jaridenv.2004.10.007
El-Keblawy A., Al-Rawai A., 2006. Effects of seed maturation time and dry storage on light and temperature requirements during germination in invasive Prosopis juliflora. Flora 201, 135-143. http://dx.doi.org/10.1016/j.flora.2005.04.009
Fenner M., 1991. The effects of the parent environment on seed germinability. Seed Sci Technol 1, 75-84.
Forcella F., Benech Arnold R.L., Sánchez R., Ghersa C.M., 2000. Modelling seedling emergence. Field Crops Res 67, 123-139. http://dx.doi.org/10.1016/S0378-4290(00)00088-5
García E.H., Pena-Valdivia C.B., Rogelio Aguirre J.R., Muruaga, J.S.M., 1997. Morphological and agronomic traits of a wild population and an improved cultivar of common bean (Phaseolus vulgaris L.). Ann Bot 79, 207-213. http://dx.doi.org/10.1006/anbo.1996.0329
Guines F., Julier B., Ecalle C., Huyghe C., 2003. Among and within-cultivar variability for histological traits of lucerne (Medicago sativa L.) stem. Euphytica 130, 293-301. http://dx.doi.org/10.1023/A:1022885320517
Huang J., Reddman R.E., 1995. Salt tolerance of Hordeum and Brassica species during germination and early seedling growth. Can J Plant Sci 75, 815-819. http://dx.doi.org/10.4141/cjps95-137
Huang Z.Y., Zhang X.S., Zheng G.H., Gutterman Y., 2003. Influence of light, temperature, salinity and storage on seed germination of Haloxylon ammodendron. J Arid Environ 55, 453-464. http://dx.doi.org/10.1016/S0140-1963(02)00294-X
Jenczewski E., Prosperi J.M., Ronfort J., 1999. Evidence for gene flow between wild and cultivated Medicago sativa (Leguminosae) based on allozyme markers and quantitative traits. Am J Bot 86, 677-687. http://dx.doi.org/10.2307/2656577 PMid:10330071
Julier B., Huyghe C., 1997. Effect of growth and cultivar on alfalfa digestibility in a multi-site trial. Agronomie 17, 481-489. http://dx.doi.org/10.1051/agro:19970905
Julier B., Huyghe C., Ecalle C., 2000. Within- and among-cultivar genetic variation in alfalfa: forage quality, morphology and yield. Crop Sci 40, 365-369. http://dx.doi.org/10.2135/cropsci2000.402365x
Katembe W.J., Ungar I.A., Mitchell J., 1998. Effect of salinity on germination and seedling growth of two Atriplex species (Chenopodiaceae). Ann Bot 82, 167-175. http://dx.doi.org/10.1006/anbo.1998.0663
Khan M.A., Ungar I.A., 1984. The effect of salinity and temperature on the germination of polymorphic seeds and growth of Atriplex triangularisWilld. Am J Bot 71, 481-489. http://dx.doi.org/10.2307/2443323
Khan M.A., Rizvi Y., 1994. Effect of salinity, temperature, and growth regulators on the germination and early seedling growth of Atriplex griffithii var. stocksii. Can J Bot 72, 475-479. http://dx.doi.org/10.1139/b94-063
Khan M.A., Ungar I.A., 1998. Seed germination and dormancy of Polygonum aviculare L. as influenced by salinity, temperature, and gibberellic acid. Seed Sci Technol 26, 107-117.
Khan M.A., Gulzar S., 2003. Germination responses of Sporobolus ioclados: a saline desert grass. J Arid Environ 53, 387-394. http://dx.doi.org/10.1006/jare.2002.1045
Khan M.A., Gul B., Weber D.J., 2002. Seed germination in relation to salinity and temperature in Sarcobatus vermiculatus. Biol Plant 45, 133-135. http://dx.doi.org/10.1023/A:1015133515568
Kiss H.G., Kiss J.Z., 1998. Spore germination in populations of Schizaea pusilla from New Jersey and NovaScotia. Int J Plant Sci 159(5), 848-852. http://dx.doi.org/10.1086/297606
Larsen S.U., Andreasen C., 2004. Light and heavy seeds differ in germination percentage and mean germination thermal time. Crop Sci 44, 1710-1720. http://dx.doi.org/10.2135/cropsci2004.1710
Lenssen A.W., Sorensen E.L., Posler G.L., Harbers L.H, 1991. Basic alfalfa germplasms differ in nutritive content of forage. Crop Sci 31, 293-296. http://dx.doi.org/10.2135/cropsci1991.0011183X003100020012x
Meyer S.E., Allen P.S., 1999. Ecological genetics of seed germination in Bromus tectorum L. II. Reaction norms in response to a water stress gradient imposed during seed maturation. Oecologia 120, 35-43. http://dx.doi.org/10.1007/s004420050830
Michaud R, Lehman W.F., Rumbaugh M.D., 1988. World distribution and historical development. In: Alfalfa and alfalfa improvement (Hanson A.A. et al., eds). ASACSSA-SSSA publishers, Agronomy monograph no. 29, Madison, WI, USA. pp. 25-91.
Mohammadi S.A., Prasanna B.M., 2003. Analysis of genetic diversity in crop plants-salient statistical tools and considerations. Crop Sci 43, 1235-1248. http://dx.doi.org/10.2135/cropsci2003.1235
ODS, 2005. Rapport annuel de l'Office de Développement du Sud Tunisien, Le gouvernorat de Tozeur, Gabes, Kebilli, Gafsa, Medenine and Tataouine. 83 pp. [In French].
Riday H., Brummer E.C., Campbell T.A., Luth D., Cazkarro P.M., 2003. Comparison of genetic and morphological distance with heterosis between Medicago sativa subsp. sativa and subsp. falcata. Euphytica 131, 37-45. http://dx.doi.org/10.1023/A:1023050126901
Rivers W.G., Weber D.J., 1971. The influence of salinity and temperature on seed germination in Salicornia bigelovii. Physiol Plantarum 24, 73-75. http://dx.doi.org/10.1111/j.1399-3054.1971.tb06719.x
Song J., Feng G., Tian C., Zhang F., 2005. Strategies for adaptation of Suaeda physophora, Haloxylon ammodendron and Haloxylon persicum to a saline environment during seed germination stage. Ann Bot 96, 399-405. http://dx.doi.org/10.1093/aob/mci196 PMid:16002418
Ungar I.A., 1982. Germination ecology of halophytes. In: Contribution to the ecology of halophytes (Sen D.N., Rajpurohit K.S., eds). Junk Hague. pp. 143-154. http://dx.doi.org/10.1007/978-94-009-8037-2_10
Ungar I.A., 1995. Seed germination and seed-bank ecology of halophytes. In: Seed development and germination (Kigel J., Galili G., eds). Marcel Dekker, NY, USA. pp. 599-627.
Ward J.H., 1963. Hierarchical grouping to optimize an objective function. Am Statist Assoc J 56, 236-244. http://dx.doi.org/10.1080/01621459.1963.10500845
Welbaum G.E., Tissaoui T., Bradford K.J., 1990. Water relations of seed development and germination in muskmelon (Cucumis melo L.) III. Sensitivity of germination to water potential and abscisic acid during development. Plant Physiol 92, 1029-1037. http://dx.doi.org/10.1104/pp.92.4.1029 PMid:16667367 PMCid:1062412
Zia S., Khan M.A., 2004. Effect of light, salinity, and temperature on seed germination of Limonium stocksii. Can J Bot 82, 151-157. http://dx.doi.org/10.1139/b03-118
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