Eficacia del hongo entomopatógeno, Metarhizium anisopliae (Metsch.) Sorokin, contra larvas de Hellula undalis, en condiciones de laboratorio

  • Malyaj R. Prajapati College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India-250110 https://orcid.org/0000-0002-8542-9732
  • Ravi Shanker Department of Entomology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India-250110
  • Reetesh P. Singh Department of Entomology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India-250110
  • Rajendra Singh Department of Entomology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India-250110
  • Pankaj Kumar Department of Entomology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India-250110 https://orcid.org/0000-0001-6632-8314
  • Jitender Singh Department of Microbiology, Chaudhary Charan Singh University, Meerut, Uttar Pradesh, India-250001 https://orcid.org/0000-0003-0715-1365
  • Mahesh K. Bharti College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India-250110 https://orcid.org/0000-0003-1558-4953
  • Lokesh K. Gangwar Department of Genetics and Plant Breeding, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India-250110 https://orcid.org/0009-0009-8932-7774
Palabras clave: agentes de control biológico, Hellula undalis, hongos entomopatógenos, Metarhizium anisopliae

Resumen

Objetivo del estudio: El objetivo de este estudio fue evaluar la eficacia de un aislado nativo de Metarhizium anisopliae (Metsch.) Sorokin como agente de biocontrol contra larvas de cuarto estadio de la polilla del repollo, Hellula undalis (Fabricius, 1781).

Área de estudio: La investigación se centró en hongos entomopatógenos y examinó particularmente el impacto de diferentes concentraciones de esporas de M. anisopliae en la mortalidad de las larvas de H. undalis bajo condiciones controladas.

Material y métodos: Las larvas de cuarto estadio de H. undalis fueron expuestas a diferentes concentraciones de esporas de M. anisopliae. Se monitorearon las tasas de mortalidad en intervalos de tiempo específicos para establecer patrones de respuesta a la dosis y determinar los tiempos letales.

Principales resultados: El estudio encontró una relación dosis-dependiente entre la concentración de esporas y la mortalidad larval. Las concentraciones más bajas inicialmente no mostraron efectos significativos, pero presentaron un aumento gradual en la mortalidad en el tiempo, mientras que las concentraciones más altas resultaron en tasas de mortalidad significativas, alcanzando el 86.88% a las 120 horas (con una variación de p ≤ 0.05.

Aspectos destacados de la investigación: Se observó una eficacia dependiente de la concentración de M: anisopliae, mostrando una disminución en el tiempo promedio de letalidad (LT50) y el tiempo para alcanzar el 90% de mortalidad (LT90) con el aumento de la concentración de esporas. Los resultados destacan el potencial de M. anisopliae como agente de control biológico contra H. undalis y proporcionan información valiosa para estrategias de control integrado de plagas. El estudio subraya las propiedades entomopatógenas de M. anisopliae y su papel como una solución de control de plagas respetuosa con el medio ambiente y sostenible.

Descargas

La descarga de datos todavía no está disponible.

Citas

Abbott WS, 1925. A method of computing the effectiveness of an insecticide. J Econ Entomol. 18(2):265-7. https://doi.org/10.1093/jee/18.2.265a

Asi MR, Bashir MH, Afzal M, Zia K, Akram M, 2013. Potential of entomopathogenic fungi for biocontrol of Spodoptera litura Fabricius (Lepidoptera: Noctuidae). J Anim Plant Sci. 23(3):913-8.

Boomsma JJ, Jensen AB, Meyling NV, Eilenberg J, 2014. Evolutionary interaction networks of insect pathogenic fungi. Annu Rev Entomol. 59:467-85. https://doi.org/10.1146/annurev-ento-011613-162054

Feng MG, Nowierski RM, Johnson JB, Poprawski TJ, 1992. Epizootics caused by entomophthoralean fungi (Zygomycetes, Entomophthorales) in populations of cereal aphids (Hom, Aphididae) in irrigated small grains of southwestern Idaho, USA. J Appl Entomol 113(1-5):376-90. https://doi.org/10.1111/j.1439-0418.1992.tb00678.x

Fite T, Tefera T, Negeri M, Damte T, Sori W, 2020. Evaluation of Beauveria bassiana, Metarhizium anisopliae, and Bacillus thuringiensis for the management of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) under laboratory and field conditions. Biocontrol Sci Technol. 30(3):278-95. https://doi.org/10.1080/09583157.2019.1707481

Finney DJ, 1971. Probit analysis. Cambridge, UK: Cambridge University Press.

Islam W, Adnan M, Shabbir A, Naveed H, Abubakar YS, Qasim M et al., 2021. Insect-fungal-interactions: A detailed review on entomopathogenic fungi pathogenicity to combat insect pests. Microb Pathog. 159:105122. https://doi.org/10.1016/j.micpath.2021.105122

Khan S, Guo L, Maimaiti Y, Mijit M, Qiu D, 2012. Entomopathogenic fungi as microbial biocontrol agent. Mol Plant Breed. 3(7). https://doi.org/10.5376/mpb.2012.03.0007

Khan BA, Nadeem MA, Nawaz H, Amin MM, Abbasi GH, Nadeem M et al., 2023. Pesticides: impacts on agriculture productivity, environment, and management strategies. In: Aftab T, editor. Emerging contaminants and plants: interactions, adaptations and remediation technologies. Cham: Springer International Publishing. p. 109-34. https://doi.org/10.1007/978-3-031-22269-6_5

Kumar A, Suroshe SS, Sonam GK, Saini GK, Singh J, 2023. Efficacy of genetically transformed Metarhizium anisopliae against Spodoptera litura and Aphis craccivora. Saudi J Biol Sci. 30(1):103493. https://doi.org/10.1016/j.sjbs.2022.103493

Kunjwal N, Srivastava RM, 2018. Insect pests of vegetables. In: Omkar, editor. Pests manag. Singapore: Springer Singapore, p. 163-221. https://doi.org/10.1007/978-981-10-8687-8_7

Labou B, Brévault T, Sylla S, Diatte M, Bordat D, Diarra K, 2017. Spatial and temporal incidence of insect pests in farmers’ cabbage fields in Senegal. Int J Trop Insect Sci. 37(4):225-33. https://doi.org/10.1017/S1742758417000200

Lin H-F, Yang X-J, Gao Y-B, Li S-G, 2007. Pathogenicity of several fungal species on Spodoptera litura. Ying Yong Sheng Tai Xue Bao. 18(4):937-40.

Nahar PB, Kulkarni SA, Kulye MS, Chavan SB, Kulkarni G, Rajendran A, Deshpande MV, 2008. Effect of repeated in vitro sub-culturing on the virulence of Metarhizium anisopliae against Helicoverpa armigera (Lepidoptera: Noctuidae). Biocontrol Sci Technol 18(4):337–55.

Nguyen NTH, Borgemeister C, Poehling H-M, Zimmermann G, 2007. Laboratory investigations on the potential of entomopathogenic fungi for biocontrol of Helicoverpa armigera (Lepidoptera: Noctuidae) larvae and pupae. Biocontrol Sci Technol. 17(8):853-64. https://doi.org/10.1080/09583150701546375

Ramanujam B, Poornesha B, Yatish KR, Renuka S, 2015. Evaluation of Pathogenicity of Different Isolates of Metarhizium anisopliae (Metchnikoff) Sorokin against maize stem borer, Chilo partellus (Swinhoe) using laboratory bioassays. Biopestic Int 11:89-95.

Rijal JP, Dhoj GCY, Thapa RB, Kafle L, 2008. Virulence of native isolates of Metarhizium anisopliae and Beauveria bassiana against Helicoverpa armigera in Nepal. Formos Entomol 28:21-9.

Sabry KH, Abdel-Raheem MA, El-Fatih MM, 2011. Efficacy of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae on some insect pests under laboratory conditions. Egypt J Biol Pest Control 21(1):33–8.

Sandhu SS, Sharma AK, Beniwal V, Goel G, Batra P, Kumar A, Malhotra S, 2012. Myco-biocontrol of insect pests: factors involved, mechanism, and regulation. J Pathog:3-12.

Shanker R, Prajapati MR, Singh RP, Singh R, Singh J, Kumar P, 2023. Isolation, molecular characterization of indigenous Metarhizium anisopliae (Metchnikoff) isolate, using ITS-5.8 s rDNA region, and its efficacy against the Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae). Egypt J Biol Pest Control. 33(1):23. https://doi.org/10.1186/s41938-023-00670-7

Shehzad M, Tariq M, Mukhtar T, Gulzar A, 2021. On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae). Egypt J Biol Pest Control 31(1):7.

Sivapragasan A, 2005. Development of cabbage webworm, Hellula undalis (Fabr.) on head cabbage, Brassica oleracea var. capitata. J Trop Agric Food Sci. 33:321-31.

Tahir HM, Basheer T, Ali S, Yaqoob R, Naseem S, Khan SY, 2019. Effect of pesticides on biological control potential of Neoscona theisi (Araneae: Araneidae). J Insect Sci 19(2):17. https://doi.org/10.1093/jisesa/iez024.

Tran TT, Le VV, Nguyen L, 2018. Study on damage situation of the cabbage webworm, Hellula undalis Fabricius (Lepidoptera: Crambidae) on green mustards at the Mekong Delta. Can Tho Univ. J. Sci. 54:115–124.

Yanar O, Topkara EF, Sahin F, Yanar Y, Yanar D, Terzi Y, 2023. Efficacy of Beauveria bassiana and Metarhizium brunneum isolates against the pine processionary moth, Thaumetopoea wilkinsoni Tams, 1926 (Lepidoptera: Notodontidae). Egypt J Biol Pest Control 33(1):32. https://doi.org/10.1186/s41938-023-00679-y.

Zaki O, Weekers F, Thonart P, Tesch E, Kuenemann P, Jacques P, 2020. Limiting factors of mycopesticide development. Biol Control 144:104220. https://doi.org/10.1016/j.biocontrol.2020.104220.

Publicado
2025-05-13
Cómo citar
Prajapati, M. R., Shanker, R., Singh, R. P., Singh, R., Kumar, P., Singh, J., Bharti, M. K., & Gangwar, L. K. (2025). Eficacia del hongo entomopatógeno, Metarhizium anisopliae (Metsch.) Sorokin, contra larvas de Hellula undalis, en condiciones de laboratorio. Spanish Journal of Agricultural Research, 23(1), 20875. https://doi.org/10.5424/sjar/2025231-20875
Sección
Protección vegetal