Influencia de las concentraciones de fertilizante de urea ecológicamente relevantes en los nematodos: Un experimento de microcosmos
Resumen
Objetivo del estudio: La urea sigue siendo uno de los fertilizantes químicos más utilizados en el mundo y se utiliza ampliamente en Filipinas como una fuente principal de nitrógeno para los cultivos. Sin embargo, tenemos una comprensión muy limitada de su impacto en la fauna del suelo, como los nematodos. En este estudio, demostramos la idoneidad del sistema de microcosmos para evaluar los efectos químicos en las comunidades de nematodos en condiciones controladas y determinamos los efectos de la urea en dichas comunidades de nematodos.
Área de estudio: Las muestras de suelo se obtuvieron de un campo relativamente “menos perturbado” en Bukidnon, Mindanao Central, y se llevaron a cabo experimentos en un invernadero en Premier Research Institute of Science and Mathematics (PRISM), Mindanao State University - Iligan Institute of Technology (MSU-IIT), Ciudad de Iligan, Filipinas.
Material y Métodos: El suelo fue recolectado y analizado para determinar sus propiedades fisiológicas. Luego, el suelo se expuso a concentraciones de urea (0 mg/kg, 19.67 mg/kg, 39.33 mg/kg y 78.66 mg/kg) en un microcosmos durante 45 días. Posteriormente, se recolectaron los nematodos y se procesaron utilizando un método de placa modificado.
Resultados principales: En primer lugar, los microcosmos naturales (sin urea) mostraron que la abundancia de nematodos y el número de géneros disminuyeron en un 28% y un 35%, respectivamente. Los resultados sugieren que la configuración actual del microcosmos puede ser útil para probar los efectos de ciertos químicos de interés. En segundo lugar, no se observaron efectos significativos en el número de géneros ni en los índices de diversidad con la urea, excepto en el día 15, cuando la abundancia de nematodos fue significativamente mayor a 39.33 mg/kg que a 19.67 mg/kg.
Aspectos destacados de la investigación: Este estudio muestra que las concentraciones de urea ecológicamente relevantes mencionadas anteriormente no tuvieron un impacto negativo en la estructura de la comunidad de nematodos durante la exposición de 45 días.
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Ahmad I, Zhu G, Zhou G, Song X, Hussein Ibrahim ME, Ibrahim Salih EG, 2022. Effect of N on growth, antioxidant capacity, and chlorophyll content of sorghum. Agronomy, 12(2). https://doi.org/10.3390/agronomy12020501
Allouche M, Nasri A, Harrath AH, Mansour L, Beyre H, Boufahj F, 2020. Migratory behavior of free-living marine nematodes surrounded by sediments experimentally contaminated by mixtures of polycyclic aromatic hydrocarbons. J King Saud Univ Sci 32(2), pp.1339-1345. https://doi.org/10.1016/j.jksus.2019.11.025
Andrássy I, 2005. Free-living Nematodes of Hungary, I (Nematoda errantia).: Vol. I. Hungarian Natural History Museum, Budapest.
Andrássy I, 2007. Free-living Nematodes of Hungary, II (Nematoda errantia).: Vol. II. Hungarian Natural History Museum, Budapest.
Andrássy I, 2009. Free-living Nematodes of Hungary, III (Nematoda errantia): Vol. III. Hungarian Natural History Museum, Budapest.
Cong Y, Yang H, Zhang P, Xie Y, Cao X, Zhang L, 2020. Transcriptome analysis of the nematode Caenorhabditis elegans in acidic stress environments. Front Psychol, 11, p.1107. https://doi.org/10.3389/fphys.2020.01107
Dan S, Hosseinkhani O, Saadat M, Bagheri H, 2024. Ammonia application in terrestrial vegetation. Progresses in Ammonia: Science, Technology and Membranes (pp. 199-221). Elsevier. https://doi.org/10.1016/B978-0-323-88501-0.00012-4
Ettema CH, Bongers T, 1993. Characterization of nematode colonization and succession in disturbed soil using the Maturity Index. Biol Fert Soils, 16, pp.79-85. https://doi.org/10.1007/BF00369407
Ewald M, Glavatska O, Ruess L, 2020. Effects of resource manipulation on nematode community structure and metabolic footprints in an arable soil across time and depth. Nematology, 22(9), pp.1025-1043. https://doi.org/10.1163/15685411-bja10009
Ewald M, Rusch D, Rißmann C, Trost B, Theuerl S, Ruess L, 2022. Effects of irrigation and fertilization practice on soil nematode communities in arable land. Appl Soil Ecol, 177, p.104546. https://doi.org/10.1016/j.apsoil.2022.104546
Ferris H, Bongers T, 2009. Indices developed specifically for analysis of nematode assemblages. Nematodes as environmental indicators, pp.124-145. https://doi.org/10.1079/9781845933852.0124
Forge T, Ehret D, Messiga A, Dorais M, 2020. Influences of nitrogen inputs on nematode populations under highbush blueberry. J Nematol, 52(1), pp.1-14. https://doi.org/10.21307/jofnem-2020-056
Greiffer L, Liebau E, Herrmann FC, Spiegler V, 2022. Condensed tannins act as anthelmintics by increasing the rigidity of the nematode cuticle. Sci Rep -UK, 12(1), p.18850. https://doi.org/10.1038/s41598-022-23566-2
Hu J, Chen G, Hassan WM, Lan J, Si W, Wang W, Li G, Du G, 2022. The impact of fertilization intensity on soil nematode communities in a Tibetan Plateau grassland ecosystem. Appl Soil Ecol, 170, p.104258. https://doi.org/10.1016/j.apsoil.2021.104258
Kacprzak, M., Malińska, K., Grosser, A., Sobik-Szołtysek, J., Wystalska, K., Dróżdż, D., Jasińska, A. and Meers, E., 2023. Cycles of carbon, nitrogen and phosphorus in poultry manure management technologies-environmental aspects. Crit Rev Env Sci Tec, 53(8), pp.914-938. https://doi.org/10.1080/10643389.2022.2096983
Karuri H, 2023. Nematode community response to intensive tomato production in the tropics. Rhizosphere, 25, p.100681. https://doi.org/10.1016/j.rhisph.2023.100681
Kharbach M, Chfadi T, 2021. General trends in fertilizer use in the world. Arab J Geosci, 14(23), p.2577. https://doi.org/10.1007/s12517-021-08889-0
Krashevska V, Kudrin AA, Widyastuti R, Scheu S, 2019. Changes in nematode communities and functional diversity with the conversion of rainforest into rubber and oil palm plantations. Front Ecol Evol 7, p.487. https://doi.org/10.3389/fevo.2019.00487
Labajo JRN, Pabiona MG, 2022. Physical and chemical properties of soil on selected sugarcane farms in Mt. Nebo, Valencia City, Bukidnon, Philippines. Asian J Agric, 6(2). https://doi.org/10.13057/asianjagric/g060204
Lazarova S, Coyne D, Rodriguez MG, Peteira B, Ciancio A, 2021. Functional diversity of soil nematodes in relation to the impact of agriculture-a review. Diversity, 13(2), p.64. https://doi.org/10.3390/d13020064
Lee EK, Zhang X, Adler PR, Kleppel GS, Romeiko XX, 2020. Spatially and temporally explicit life cycle global warming, eutrophication, and acidification impacts from corn production in the US Midwest. J Clean Prod, 242, p.118465. https://doi.org/10.1016/j.jclepro.2019.118465
Li C, Wang X, Chen B, Wang L, Xie Z, Wang J, Yang Z, 2023. Fertilization restructures nematode assemblages by modifying soil pH in croplands of Northeast China. Front Environ Sci, 11, 1207379. https://doi.org/10.3389/fenvs.2023.1207379
Li J, Peng P, Zhao J, 2020. Assessment of soil nematode diversity based on different taxonomic levels and functional groups. Soil Ec Lett, 2(1), pp.33-39. https://doi.org/10.1007/s42832-019-0019-5
Liang S, Kou X, Li Y, Lü X, Wang J, Li Q, 2020. Soil nematode community composition and stability under different nitrogen additions in a semiarid grassland. Global Ecol Conserv, 22, p.e00965. https://doi.org/10.1016/j.gecco.2020.e00965
Liu J, Chen Y, Du C, Liu X, Ma Q, Zhang X, Wang D, 2019. Interactive effects of nitrogen addition and litter on soil nematodes in grassland. EUR J SOIL SCI, 70(3), pp.697-706. https://doi.org/10.1111/ejss.12779
Liu T, Wang Z, Guan H, Zhong B, He X, Wang Y, Qi Y, Yan W, Lu X, 2023. Soil Macrofauna Disperse and Reconstruct Soil Nematode Communities: Takeaways from a Microcosm Study. Forests, 14(4), p.748. https://doi.org/10.3390/f14040748
Lu Q, Liu T, Wang N, Dou Z, Wang K, Zuo Y, 2020. A review of soil nematodes as biological indicators for the assessment of soil health. Front. Agric Sci Eng 7, pp.275-281. https://doi.org/10.15302/J-FASE-2020327
Martikainen E, Haimi J, Ahtiainen J, 1998. Effects of dimethoate and benomyl on soil organisms and soil processes-a microcosm study. Appl Soi Ecol, 9(1-3), 381-387. https://doi.org/10.1016/S0929-1393(98)00093-6
Martinez JG, Torres MA, dos Santos G, Moens T, 2018. Influence of heavy metals on nematode community structure in deteriorated soil by gold mining activities in Sibutad, southern Philippines. Ecol Indic, 91, pp.712-721. https://doi.org/10.1016/j.ecolind.2018.04.021
Martinez JG, Quiobe SP, Moens T, 2019. Effects of mercury (Hg) on soil nematodes: A microcosm approach. Arch Environ Con Tox, 77, pp.421-431. https://doi.org/10.1007/s00244-019-00652-7
Mayrhofer N, Velicer GJ, Schaal KA, Vasse M, 2021. Behavioral interactions between bacterivorous nematodes and predatory bacteria in a synthetic community. Microorganisms, 9(7), p.1362. https://doi.org/10.3390/microorganisms9071362
McQueen JP, Gendron EM, Solon AJ, de Mesquita CPB, Hufft RA, Shackelford N, Suding KN, Schmidt SK, Porazinska DL, 2024. Glyphosate-based restoration of a degraded grassland threatens soil health and the diversity of nematode communities. Soil Biol Biochem, p.109350. https://doi.org/10.1016/j.soilbio.2024.109350
Moura GS, Franzener G, 2017. Biodiversity of nematodes biological indicators of soil quality in the agroecosystems. Arquivos do Instituto Biológico, 84, p.e0142015. https://doi.org/10.1590/1808-1657000142015
Ni X, Zhu X, Feng Q, Zhao D, Huang W, Pan F, 2024. Effect of Application Rates of N and P Fertilizers on Soil Nematode Community Structure in Mollisols. Agronomy, 14(3), p.507. https://doi.org/10.3390/agronomy14030507
Nisa RU, Tantray AY, Kouser N, Allie KA, Wani SM, Alamri SA, Alyemeni MN, Wijaya L, Shah AA, 2021. Influence of ecological and edaphic factors on biodiversity of soil nematodes. Saudi J Biol Sci, 28(5), pp.3049-3059.https://doi.org/10.1016/j.sjbs.2021.02.046
Pothula SK, Phillips G, Bernard EC, 2022. Increasing levels of physical disturbance affect soil nematode community composition in a previously undisturbed ecosystem. J Nematol, 54(1). https://doi.org/10.2478/jofnem-2022-0022
Siddiqi MR, 1997. Techniques and methodologies for nematode disease diagnosis and nematode identification. FAO Plant P, 144, pp.21-44.
Sieriebriennikov B, Ferris H, de Goede RG, 2014. NINJA: An automated calculation system for nematode-based biological monitoring. Eur J Soil Biol, 61, pp.90-93. https://doi.org/10.1016/j.ejsobi.2014.02.004
Shokoohi E, 2024. Interactions of Free-Living Nematodes and Associated Microorganisms with Plant-Parasitic Nematodes. Sustainable Management of Nematodes in Agriculture, Vol. 2: Role of Microbes-Assisted Strategies (pp. 127-147). https://doi.org/10.1007/978-3-031-52557-5_5
Staley C, Breuillin-Sessoms F, Wang P, Kaiser T, Venterea RT, Sadowsky MJ, 2018. Urea amendment decreases microbial diversity and selects for specific nitrifying strains in eight contrasting agricultural soils. Front Microbiol, 9, p.327392. https://doi.org/10.3389/fmicb.2018.00634
Sun Z, Sun C, Feng X, Zhang T, Liu J, Wang X, Li S, Tang S, Jin K, 2024. Grazing alters the soil nematode communities in grasslands: A meta-analysis. J Environ Manage, 356, p.120668. https://doi.org/10.1016/j.jenvman.2024.120668
Teshita A, Feng Y, Qian R, Wang X, Khan W, Gao Y, 2023. Alfalfa and maize intercropping enhances soil nematode structure and food web complexity in low-nitrogen soils. Appl Soil Ecol, 186, p.104809. https://doi.org/10.1016/j.apsoil.2023.104809
Traunspurger W, Majdi N, 2017. Meiofauna. In Methods in Stream Ecology, Volume 1 (pp. 273-295). Academic Press. https://doi.org/10.1016/B978-0-12-416558-8.00014-7
Wang J, Wang H, Lin Q, Wu Y, He X, Chen X, Yan W, Zhao J, 2023a. Legume biological nitrogen fixation improves but chemical nitrogen fertilizer suppresses soil nematode communities in a Camellia oleifera plantation. Land Degrad Dev, 34(5), pp.1403-1414. https://doi.org/10.1002/ldr.4542
Wang J, Zhao X, Wei K, Tang J, Yuan C, Jin B, Sun X, Zhu B, 2023b. Correlations Between the Soil Bacterial-Feeding Nematodes, Bacteria, and Nitrogen in the Cropland of the Upper Yangtze River, China. J Soil Sci Plant Nut, 23(4), pp.5840-5849. https://doi.org/10.1007/s42729-023-01443-9
Wang Y, Huang B, Yan G, Liu G, Xing Y, Wang Q, 2022. Effects of long-term nitrogen addition and seasonal variation on soil faunal community structure in a temperate natural secondary forest. Eur J For Res, 141(4), pp.547-560. https://doi.org/10.1007/s10342-022-01457-5
Wilschut RA, Geisen S, 2021. Nematodes as drivers of plant performance in natural systems. Trends Plant Sci, 26(3), pp.237-247. https://doi.org/10.1016/j.tplants.2020.10.006
Zhang B, Zhang L, Zhang X, 2019. Bioremediation of petroleum hydrocarbon-contaminated soil by petroleum-degrading bacteria immobilized on biochar. Rsc Adv, 9(60), pp.35304-35311. https://doi.org/10.1039/C9RA06726D
Zhang H, Tian M, Jiang M, Yang J, Xu Q, Zhang Y, Ji M, Yao Y, Zhao C, Miao Y, 2024. Effects of nitrogen and phosphorus additions on soil nematode community of soybean farmland. Soil Ecol Lett. 6(2), p.230200. https://doi.org/10.1007/s42832-023-0200-8
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