La luz solar y la iluminación LED y HPS modulan la respuesta de Bacillus mycoides al estrés por sombra en raigrás perenne

Palabras clave: Bacillus mycoides, césped deportivo, estrés por sombra, iluminación LED, iluminación suplementaria, PGPR, raigrás perenne

Resumen

Objetivo del estudio: Investigar los efectos combinados de las tecnologías de iluminación suplementaria, diodo emisor de luz (LED) y sodio de alta presión (HPS), los cultivares de raigrás perenne, y la inoculación con Bacillus mycoides en el establecimiento temprano del césped bajo condiciones de sombra similares a las de un estadio, abordando los plazos críticos de renovación en recintos deportivos profesionales. Área de estudio: Instalaciones de invernadero en la Escuela Vocacional de Bayındır de la Universidad de Ege, Izmir, Turquía. Material y métodos: Tres cultivares de raigrás perenne, Lolium perenne L. ‘Presidian’, Lolium perenne L. ‘Apple SGL’ y Lolium × boucheanum Kunth. ‘Solstice II’, se cultivaron en arena lavada (0–2 mm) bajo cuatro ambientes lumínicos: luz solar, 70% de sombra, sombra + LED y sombra + HPS. La cepa BM02 de B. mycoides se aplicó a una dosis de 2 L ha⁻¹ en la mitad de los tratamientos. Ambos sistemas de iluminación suplementaria proporcionaron 100 μmol m⁻² s⁻¹ de densidad de flujo fotónico fotosintético. Los parámetros medidos incluyeron cobertura del tapiz, tasa de crecimiento, desarrollo radicular, índice de color verde oscuro (DGCI) y contenido de clorofila durante cinco semanas, utilizando un diseño factorial 4 × 3 × 2 con cuatro repeticiones. Resultados principales: Las interacciones significativas de tres factores dominaron todas las variables de respuesta. La sombra produjo el rendimiento más bajo en todos los parámetros. La iluminación LED alcanzó una cobertura del tapiz cercana a los niveles de luz solar (43.5–51.2% frente a 48.6–57.3%), mientras que HPS resultó en una cobertura significativamente menor (20.2–28.3%) a pesar de promover el crecimiento vertical. Bacillus mycoides incrementó la cobertura del tapiz (5.8–21.3%) y el peso fresco radicular (23.4–28.5%) bajo iluminación suplementaria, pero redujo la cobertura del tapiz (11.3–12.8%) y el índice de color verde oscuro (DGCI) (11.3–24.2%) bajo condiciones de luz solar. Aspectos destacados de la investigación: La iluminación LED proporciona resultados superiores en el establecimiento con un ahorro energético del 40% en comparación con HPS. Los beneficios de B. mycoides se maximizan bajo condiciones de estrés más que en ambientes óptimos.

Descargas

La descarga de datos todavía no está disponible.

Citas

Abélard E, Galbrun C, 2022. The effects of artificial lighting on sports turf. Int Turfgrass Soc Res J 14(1): 1016–1021. https://doi.org/10.1002/its2.115

Ali B, Wang X, Saleem MH, Azeem MA, Afridi MS, Nadeem M, Ghazal M, Batool T, Qayyum A, Alatawi A et al., 2022. Bacillus mycoides pm35 reinforces photosynthetic efficiency, antioxidant defense, expression of stress-responsive genes, and ameliorates the effects of salinity stress in maize. Life 12(2): 219. https://doi.org/10.3390/life12020219

Allard G, Nelson CJ, Pallardy SG, 1991. Shade effects on growth of tall fescue: I. leaf anatomy and dry matter partitioning. Crop Sci 31(1): 163–167. https://doi.org/10.2135/cropsci1991.0011183X003100010037x

Bailey S, Walters RG, Jansson S, Horton P, 2001. Acclimation of Arabidopsis thaliana to the light environment: the existence of separate low light and high light responses. Planta 213(5): 794–801. https://doi.org/10.1007/s004250100556

Baker SW, 1995. The effects of shade and changes in microclimate on the quality of turf at professional football clubs. I. Questionnaire survey. J Sports Turf Res Inst (71): 66–74

Bell GE, 2011. Turfgrass physiology and ecology: advanced management principles. 1st edn. CABI, Wallingford, UK.

Brito C, Ferreira H, Dinis L-T, Trindade H, Marques D, Correia CM, Moutinho-Pereira J, 2023. Different LED light intensity and quality change perennial ryegrass (Lolium perenne L.) physiological and growth responses and water and energy consumption. Front Plant Sci 14: 1160100. https://doi.org/10.3389/fpls.2023.1160100

Dąbrowski P, Pawluśkiewicz B, Baczewska AH, Oglęcki P, Kalaji H, 2015. Chlorophyll a fluorescence of perennial ryegrass (Lolium perenne L.) varieties under long term exposure to shade. Zemdirb-Agric 102(3): 305–312. https://doi.org/10.13080/z-a.2015.102.039

Du Jardin P, 2015. Plant biostimulants: Definition, concept, main categories and regulation. Sci Hortic 196: 3–14. https://doi.org/10.1016/j.scienta.2015.09.021

Fan Q, Jespersen D, 2025. Elucidating UV-C-induced growth inhibition in seashore paspalum and bermudagrass from photosynthesis and phytohormone changes. J Plant Growth Regul 44: 3569–3578. https://doi.org/10.1007/s00344-025-11629-3

Fu J, Luo Y, Sun P, Gao J, Zhao D, Yang P, Hu T, 2020. Effects of shade stress on turfgrasses morphophysiology and rhizosphere soil bacterial communities. BMC Plant Biol 20(1): 92. https://doi.org/10.1186/s12870-020-2300-2

Gardner DS, Goss RM, 2013. Management of turfgrass in shade. Turfgrass: Biology, use, and management; Stier JC, Horgan BP, Bonos SA (eds). pp: 219–247. American Society of Agronomy, Madison, WI, USA. https://doi.org/10.2134/agronmonogr56.c6

Ihtisham M, Hasanuzzaman M, El-Sappah AH, Zaman F, Khan N, Raza A, Sarraf M, Khan S, Abbas M, Hassan MJ et al., 2023. Primary plant nutrients modulate the reactive oxygen species metabolism and mitigate the impact of cold stress in overseeded perennial ryegrass. Front Plant Sci 14: 1149832. https://doi.org/10.3389/fpls.2023.1149832

Islam MA, Kuwar G, Clarke JL, Blystad D-R, Gislerød HR, Olsen JE, Torre S, 2012. Artificial light from light emitting diodes (LEDs) with a high portion of blue light results in shorter poinsettias compared to high pressure sodium (HPS) lamps. Sci Hortic 147: 136–143. https://doi.org/10.1016/j.scienta.2012.08.034

Karcher DE, Purcell CJ, Richardson MD, Purcell LC, Hignight KW, 2017. A new java program to rapidly quantify several turfgrass parameters from digital images. Proc ASA, CSSA and SSSA Int Annu Meet, Tampa (USA), Oct 22–25. Abstract no. 109313.

Katzin D, Marcelis LFM, van Mourik S, 2021. Energy savings in greenhouses by transition from high-pressure sodium to LED lighting. Appl Energy 281: 116019. https://doi.org/10.1016/j.apenergy.2020.116019

Kennedy E, O’Donovan M, 2014. Early season dry matter production of three hybrid ryegrass (Lolium boucheanum) and two perennial ryegrass (Lolium perenne) cultivars. Grass Forage Sci 69(3): 425–430. https://doi.org/10.1111/gfs.12062

Klein M, Stewart JD, Porter SS, Weedon JT, Kiers ET, 2022. Evolution of manipulative microbial behaviors in the rhizosphere. Evol Appl 15(10): 1521–1536. https://doi.org/10.1111/eva.13333

Ku Y, Liao Y, Chiou S, Lam H, Chan C, 2024. From trade-off to synergy: microbial insights into enhancing plant growth and immunity. Plant Biotechnol J 22(9): 2461–2471. https://doi.org/10.1111/pbi.14360

Kulikova EG, Efremova SYu, Politaeva N, Smyatskaya Y, 2019. Efficiency of an alternative LED-based grow light system. IOP Conf Ser Earth Environ Sci 288(1): 012064. https://doi.org/10.1088/1755-1315/288/1/012064

Kumar D, Ali Mohd, Sharma N, Sharma R, Manhas RK, Ohri P, 2024. Unboxing PGPR-mediated management of abiotic stress and environmental cleanup: what lies inside?. Environ Sci Pollut Res 31(35): 47423–47460. https://doi.org/10.1007/s11356-024-34157-1

Kumar R, Yer H, Li W, Jiang X, Gai Y, Duan H, Li Y, 2024. Molecular and physiological characterization of tillering and shade tolerance of dwarf mutants of perennial ryegrass. bioRxiv 2024.08.18.606542. https://doi.org/10.1101/2024.08.18.606542

Kurniawan A, Chuang H, 2022. Rhizobacterial Bacillus mycoides functions in stimulating the antioxidant defence system and multiple phytohormone signalling pathways to regulate plant growth and stress tolerance. J Appl Microbiol 132(2): 1260–1274. https://doi.org/10.1111/jam.15252

Li W, Song Y-L, Zhang Y-D, Guo S-X, 2024. PGPR alleviated the negative effects of low temperature and low light stress on the growth and physiology of violet plants. Not Bot Horti Agrobot Cluj-Napoca 52(3): 13273. https://doi.org/10.15835/nbha52313273

Lokstein H, Renger G, Götze J, 2021. Photosynthetic light-harvesting (antenna) complexes—structures and functions. Molecules 26(11): 3378. https://doi.org/10.3390/molecules26113378

Mesquita AFN, Bandeira LL, Neto JMM, Martins SCS, Martins CM, 2023. Co-inoculation of rhizobia and other plant growth-promoting rhizobacteria as biofertilizers: A biotechnological overview. Int J Adv Biotechnol Res 14(1): 1–16. https://doi.org/10.5281/ZENODO.7558384

Ouzounis T, Rosenqvist E, Ottosen C-O, 2015. Spectral effects of artificial light on plant physiology and secondary metabolism: A review. HortScience 50(8): 1128–1135. https://doi.org/10.21273/HORTSCI.50.8.1128

Patrignani A, Ochsner TE, 2015. Canopeo: A powerful new tool for measuring fractional green canopy cover. Agron J 107(6): 2312–2320. https://doi.org/10.2134/agronj15.0150

Satapute PP, Olekar HS, Shetti AA, Kulkarni AG, Hiremath GB, Patagundi BI, Shivsharan CT, Kaliwal BB, 2012. Isolation and characterization of nitrogen fixing Bacillus subtilis strain as-4 from agricultural soil. Int J Recent Sci Res 3: 762–765.

Taiz L, Zeiger E, Moller IM, Murphy A, 2015. Plant physiology and development, 6th ed. Sinauer Assoc, Inc., Sunderland, MA, USA.

Tsotetsi T, Nephali L, Malebe M, Tugizimana F, 2022. Bacillus for plant growth promotion and stress resilience: what have we learned?. Plants 11(19): 2482. https://doi.org/10.3390/plants11192482

Turnbull A, 2021. Investigating the exogenous application of 5-aminolevulinic acid to improve turfgrass (Lolium perenne L.) surfaces grown in shade. Master’s thesis. University of Nottingham, Nottingham, UK.

Uddin MK, Juraimi AS, Ismail MR, Hossain MA, Othman R, Abdul Rahim A, 2012. Physiological and growth responses of six turfgrass species relative to salinity tolerance. Sci World J 2012: 1–10. https://doi.org/10.1100/2012/905468

Walters RG, 2004. Towards an understanding of photosynthetic acclimation. J Exp Bot 56(411): 435–447. https://doi.org/10.1093/jxb/eri060

Walton TE, McCalla JH, Karcher DE, Hutchens WJ, Chandra A, Richardson MD, 2025. Shade, height of cut, and plant growth regulator effects on bermudagrass and zoysiagrass putting greens. Crop Sci 65(1): e21394. https://doi.org/10.1002/csc2.21394

Wang G, Mao J, Ji M, Wang W, Fu J, 2024. A comprehensive assessment of photosynthetic acclimation to shade in C4 grass (Cynodon dactylon (L.) Pers.). BMC Plant Biol 24(1): 591. https://doi.org/10.1186/s12870-024-05242-x

Wherley BG, Gardner DS, Metzger JD, 2005. Tall Fescue photomorphogenesis as influenced by changes in the spectral composition and light intensity. Crop Sci 45(2): 562–568. https://doi.org/10.2135/cropsci2005.0562

Xie F, Shi Z, Zhang G, Zhang C, Sun X, Yan Y, Zhao W, Guo Z, Zhang L, Fahad S, et al., 2020. Quantitative leaf anatomy and photophysiology systems of C3 and C4 turfgrasses in response to shading. Sci Hortic 274: 109674. https://doi.org/10.1016/j.scienta.2020.109674

Yunze S, Shuangsheng G, 2014. Effects of photoperiod on wheat growth, development and yield in CELSS. Acta Astronaut 105(1): 24–29. https://doi.org/10.1016/j.actaastro.2014.08.024

Zhang K, Xie H, Wen J, Zhang J, Wang Z-Y, Xu B, Chai M, 2024. Leaf senescence in forage and turf grass: progress and prospects. Grass Res 4: e004. https://doi.org/10.48130/grares-0024-0002

Zhang L, Huang X, Liu Y, Ma N, Li D, Hu Q, Zhang W, Wang K, 2024. MicroRNA164 regulates perennial ryegrass (Lolium perenne L.) adaptation to changing light intensity. Agronomy 14(6): 1142. https://doi.org/10.3390/agronomy14061142

Zhang Q, Rue K, 2024. Effects of plant growth-promoting microorganisms on the early growth of kentucky bluegrass under drought and salinity. HortTechnology 34(4): 405–411. https://doi.org/10.21273/HORTTECH05366-23

Publicado
2026-05-27
Cómo citar
Balekoğlu, E., Salman, A., & Yağmur, B. (2026). La luz solar y la iluminación LED y HPS modulan la respuesta de Bacillus mycoides al estrés por sombra en raigrás perenne. Spanish Journal of Agricultural Research, 24(1), 21909. https://doi.org/10.5424/sjar/2026241-21909
Sección
Medio ambiente y ecología agraria