La reiteración de la biodesinfección controla la incidencia de la podredumbre radicular y del cuello del pimiento causada por Phytophthora y mejora la calidad del suelo

  • M. Nuñez-Zofío Department of Plant Production and Protection, NEIKER-Tecnalia, Basque Institute of Agricultural Research and Development, Berreaga 1, 48160 Derio
  • S. Larregla del Palacio Department of Plant Production and Protection, NEIKER-Tecnalia, Basque Institute of Agricultural Research and Development, Berreaga 1, 48160 Derio
  • C. Garbisu Soil Microbial Ecology Group, NEIKER-Tecnalia, Derio
Palabras clave: biofumigación, calidad del suelo, enmienda orgánica, fitopatógenos telúricos, propiedades microbianas edáficas, solarización, supresividad

Resumen

La podredumbre radicular y del cuello causada por Phytophthora es una enfermedad que genera importantes pérdidas económicas en cultivos de pimiento en invernadero. En una región de clima templado (norte de España) se realizó ensayo en invernadero para evaluar la reiteración de tratamientos de biodesinfección después de tres ciclos de cultivo consecutivos con diferentes enmiendas orgánicas (mezcla no-compostada de estiércol de oveja y gallinaza, mezcla semicompostada de estiércol de caballo y gallinaza, pellets deshidratados de Brassica carinata más Sinapis alba como abono verde fresco) sobre la incidencia de la enfermedad, la producción y la calidad del suelo. La biodesinfección mejoró las propiedades hídricas del suelo al reducir la densidad aparente y aumentar la infiltración. Los suelos biodesinfectados mostraron valores más altos en las propiedades físico-químicas y microbianas que los suelos control (no tratado) y acolchado con plástico. En las enmiendas no-compostada y semicompostada, los valores más altos de actividad microbiana estuvieron fuertemente relacionados con un aumento en la biomasa microbiana. La biodesinfección con Brassica-Sinapis tuvo menor efecto sobre las propiedades del suelo que con ambas enmiendas animales. No obstante, los recuentos de bacterias totales, actinomicetos y Pseudomonas spp. fueron más altos en los suelos tratados con Brassica-Sinapis. La biodesinfección reiterada para controlar la podredumbre radicular y del cuello causada por Phytophthora en cultivos de pimiento en invernadero en regiones de clima templado, puede mejorar la calidad del suelo y su supresividad, así como reducir dosis de enmienda requerida. La enmienda semicompostada fue la mejor en disminuir la incidencia de enfermedad.

Descargas

La descarga de datos todavía no está disponible.

Biografía del autor/a

M. Nuñez-Zofío, Department of Plant Production and Protection, NEIKER-Tecnalia, Basque Institute of Agricultural Research and Development, Berreaga 1, 48160 Derio

Departamento de Producción y Protección Vegetal

Becario INIA predoctoral

S. Larregla del Palacio, Department of Plant Production and Protection, NEIKER-Tecnalia, Basque Institute of Agricultural Research and Development, Berreaga 1, 48160 Derio

Departamento de Producción y Protección Vegetal 

Investigador Asociado

 

C. Garbisu, Soil Microbial Ecology Group, NEIKER-Tecnalia, Derio

Soil Microbial Ecology Group  

Departamento de Ecología y Recursos Naturales 

Director Científico de la Unidad de Medio Ambiente y Recursos Naturales

 

Citas

Arriaga H, Nez-Zofo M, Larregla S, Merino P, 2011. Gaseous emissions from soil biodisinfestation by animal manure on a greenhouse pepper crop. Crop Prot 30: 412-419.
http://dx.doi.org/10.1016/j.cropro.2010.12.012

Aryantha IP, Cross R, Guest DI, 2000. Suppression of Phytophthora cinnamomi in potting mixes amended with uncomposted and composted animal manures. Phytopathology 90: 775-782.
http://dx.doi.org/10.1094/PHYTO.2000.90.7.775
PMid:18944498

Beyer L, Wachendorf C, Elsner C, Knabe R, 1993. Suitability of dehydrogenase activity assay as an index of soil biological activity. Biol Fert Soils 16: 52-56.
http://dx.doi.org/10.1007/BF00336515

Boehm MJ, Wu T, Stone AG, Kraakman B, Iannotti DA, Wilson GE, Madden LV, Hoitink HAJ, 1997. Cross-polarized magic-angle spinning 13C nuclear magnetic resonance spectroscopic characterization of soil organic matter relative to culturable bacterial species composition and sustained biological control of Pythium root rot. Appl Environ Microbiol 63: 162-168.
PMid:16535481 PMCid:1389096

Bonanomi G, Antignani V, Pane C, Scala F, 2007. Suppression of soilborne fungal diseases with organic amendments. J Plant Pathol 89: 311-324.

Bonanomi G, Antignani V, Capodilupo M, Scala F, 2010. Identifying the characteristics of organic soil amendments that suppress soil-borne plant diseases. Soil Biol Biochem 42: 136-144.
http://dx.doi.org/10.1016/j.soilbio.2009.10.012

Coelho L, Chellemi DO, Mitchell DJ, 1999. Efficacy of soil solarization and cabbage amendment for the control of Phytophthora spp. in North Florida. Plant Dis 83: 293-299.
http://dx.doi.org/10.1094/PDIS.1999.83.3.293

Correa OS, Montecchia MS, Berti MF, Fernndez-Ferrari MC, Pucheu NL, Kerber NL, Garca AF, 2009. Bacillus amyloliquefaciens BNM122, a potential biocontrol agent applied on soybean seeds, causes a minor impact on rhizosphere and soil microbial communities. Appl Soil Ecol 41: 185-194.
http://dx.doi.org/10.1016/j.apsoil.2008.10.007

De Leij FAAM, Whipps JM, Lynch JM, 1993. The use of colony development for the characterization of communities in soil and roots. Microb Ecol 27: 81-97.

Du Fretay G, Dasque J, Auger J, Coosemans J, Colla P, Pauwells F, Fritsch J, 2010. ALTERBROMIDE: dissemination of sustainable alternatives to methyl bromide in soil disinfestation and in post harvest in Europe. Acta Hortic 883: 113-120.

Epelde L, Becerril JM, Hernndez-Allica J, Barrutia O, Garbisu C, 2008. Functional diversity as indicator of the recovery of soil health derived from Thlaspi caerulescens growth and metal phytoextraction. Appl Soil Ecol 39: 299-310.
http://dx.doi.org/10.1016/j.apsoil.2008.01.005

Epelde L, Becerril JM, Kowalchuk GA, Deng Y, Zhou JZ, Garbisu C, 2010. Impact of metal pollution and Thlaspi caerulescens growth on soil microbial communities. Appl Environ Microbiol 76: 7843-7853.
http://dx.doi.org/10.1128/AEM.01045-10
PMid:20935131 PMCid:2988604

Etxeberria A, Mendarte S, Larregla S, 2011a. Determination of viability of Phytophthora capsici oospores with the tetrazolium bromide staining test versus a plasmolysis method. Rev Iberoam Micol 28(1): 43-49.
http://dx.doi.org/10.1016/j.riam.2010.11.005
PMid:21167295

Etxeberria A, Mendarte S, Larregla S, 2011b. Thermal inactivation of Phytophthora capsici oospores. Rev Iberoam Micol 28(2): 83-90.
http://dx.doi.org/10.1016/j.riam.2011.01.004
PMid:21352945

Gamliel A, Austerweil M, Kritzman G, 2000. Non-chemical approach to soilborne pest management-organic amendments. Crop Prot 19: 847-853.
http://dx.doi.org/10.1016/S0261-2194(00)00112-5

Grossman RB, Reinsch TG, 2002. Bulk density and linear extensibility. In: Methods of soil analysis, Part 4. Physical methods (Dane JH, Topp GC, eds.). Soil Sci Soc Am, Madison, pp: 2012-2028.

Grnwald NJ, Workneh F, Hu S, Van Bruggen AHC, 1997. Comparison of an in vitro and damping-off assay to test soils for suppressiveness to Pythium aphanidermatum. Eur J Plant Pathol 103: 55-63.
http://dx.doi.org/10.1023/A:1008603006217

Hoitink HAJ, Boehm MJ, Hadar Y, 1993. Mechanisms of suppression of soil-borne plant pathogens in compost-amended substrates. In: Science and engineering of composting: design, environmental, microbiological and utilization aspects (Hoitink HAJ, Keener HM eds.). Renaissance Publ, Worthington, OH, USA, pp: 601-621.

Janvier C, Villeneuve F, Alabouvette C, Edel-Hermann V, Mateille T, Steinberg C, 2007. Soil health through soil disease suppression: which strategy from descriptors to indicators? Soil Biol Biochem 39: 1-23.
http://dx.doi.org/10.1016/j.soilbio.2006.07.001

Kandeler E, Gerber H, 1988. Short-term assay of soil urease activity using colorimetric determination of ammonium. Biol Fertil Soils 6: 68-72.
http://dx.doi.org/10.1007/BF00257924

Kostiakov AN, 1932. On the dynamics of the coefficient of water percolation in soils and on the necessity of studying it from a dynamic point of view for purposes of amelioration. Russian Part A [Translation of the 6th Cong of the Int Soc Soil Sci], pp: 17-21.

Kozdrj J, Trevors JT, Van Elsas JD, 2004. Influence of introduced potential biocontrol agents on maize seedling growth and bacterial community structure in the rhizosphere. Soil Biol Biochem 36: 1775-1784.
http://dx.doi.org/10.1016/j.soilbio.2004.04.034

Litterick AM, Harrier L, Wallace P, Watson CA, Wood M, 2004. The role of uncomposted materials, composts, manures, and compost extracts in reducing pest and disease incidence and severity in sustainable temperate agricultural and horticultural crop production-A review. Crit Rev Plant Sci 23: 453-479.
http://dx.doi.org/10.1080/07352680490886815

Liu B, Gumpertz ML, Hu SJ, Ristaino JB, 2008. Effect of prior tillage and soil fertility amendments on dispersal of Phytophthora capsici and infection of pepper. Eur J Plant Pathol 120: 273-287.
http://dx.doi.org/10.1007/s10658-007-9216-7

Mandal A, Patra AK, Singh D, Swarup A, Masto RE, 2007. Effect of long-term application of manure and fertilizer on biological and biochemical activities in soil during crop development stages. Bioresour Technol 98: 3585-3592.
http://dx.doi.org/10.1016/j.biortech.2006.11.027
PMid:17207997

MAPA, 1994. Mtodos oficiales de anlisis de suelos y aguas para riego. In: Mtodos Oficiales de Anlisis, Vol. III. Ministerio de Agricultura, Pesca y Alimentacin, Madrid. [In Spanish].

Martens DA, Frankenberger WT, 1992. Modification of infiltration rates in an organic-amended irrigated soil. Agron J 84: 707-717.
http://dx.doi.org/10.2134/agronj1992.00021962008400040032x

Mijangos I, Prez R, Albizu I, Garbisu C, 2006. Effects of fertilization and tillage on soil biological parameters. Enzym Microb Technol 40: 100-106.
http://dx.doi.org/10.1016/j.enzmictec.2005.10.043

Mijangos I, Becerril JM, Albizu I, Epelde L, Garbisu C, 2009. Effects of glyphosate on rhizosphere soil microbial communities under two different plant compositions by cultivation-dependent and -independent methodologies. Soil Biol Biochem 41: 505-513.
http://dx.doi.org/10.1016/j.soilbio.2008.12.009

Mijangos I, Albizu I, Garbisu C, 2010. Beneficial effects of organic fertilization and no-tillage on fine-textured soil properties under two different forage crop rotations. Soil Sci 175: 173-185.
http://dx.doi.org/10.1097/SS.0b013e3181dd51ba

Moral R, Paredes C, Bustamante MA, Marhuenda-Egea F, Bernal MP, 2009. Utilisation of manure composts by high-value crops: Safety and environmental challenges. Bioresour Technol 100: 5454-5460.
http://dx.doi.org/10.1016/j.biortech.2008.12.007
PMid:19157868

Nez-Zofo M, Larregla S, Garbisu C, 2011. Application of organic amendments followed by soil plastic mulching reduces the incidence of Phytophthora capsici in pepper crops under temperate climate. Crop Prot 30: 1563-1572.
http://dx.doi.org/10.1016/j.cropro.2011.08.020

OJ, 1991. Directive 91/676/EEC of the Council of the European Communities of December 12 concerning the protection of waters against pollution caused by nitrates from agricultural sources. Official Journal of the European Union L 375, 31/12/1991. p. 1-8.

Oka Y, 2010. Mechanisms of nematode suppression by organic soil amendments: a review. Appl Soil Ecol 44: 101-115.
http://dx.doi.org/10.1016/j.apsoil.2009.11.003

Powers RF, 1980. Mineralizable soil nitrogen as an index of nitrogen availability to forest trees. Soil Sci Soc Am J 44: 1314-1320.
http://dx.doi.org/10.2136/sssaj1980.03615995004400060037x

Ricci P, 1972. Moyens d'tude de l'inoculum du Phytophthora nicotianae f. sp. parasitica (Dastur) Waterh parasite de I'oeillet dans le sole. Annuale Phytopathologie 4, 257-276. [In French].

Rietz DN, Haynes RJ, 2003. Effects of irrigation-induced salinity and sodicity on soil microbial activity. Soil Biol Biochem 35: 845-854.
http://dx.doi.org/10.1016/S0038-0717(03)00125-1

Rodrguez-Loinaz G, Onaindia M, Amezaga I, Mijangos I, Garbisu C, 2008. Relationship between vegetation diversity and soil functional diversity in native mixed-oak forests. Soil Biol Biochem 40: 49-60.
http://dx.doi.org/10.1016/j.soilbio.2007.04.015

Ros M, Garca C, Hernndez MT, Lacasa A, Fernndez P, Pascual JA, 2008. Effects of biosolarization as methyl bromide alternative for Meloidogyne incognita control on quality of soil under pepper. Biol Fert Soils 45: 37-44.
http://dx.doi.org/10.1007/s00374-008-0307-1

Schnrer J, Rosswall T, 1982. Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter. Appl Environ Microbiol 43: 1256-1261.
PMid:16346026 PMCid:244223

Taylor JP, Wilson B, Mills MS, Burns RG, 2002. Comparison of microbial numbers and enzymatic activities in surface soils and subsoils using various techniques. Soil Biol Biochem 34: 387-401.
http://dx.doi.org/10.1016/S0038-0717(01)00199-7

Tenuta M, Lazarovits G, 2002. Ammonia and nitrous acid from nitrogenous amendments kill the microsclerotia of Verticillium dahliae. Phytopathology 92: 255-264.
http://dx.doi.org/10.1094/PHYTO.2002.92.3.255
PMid:18943996

Tenuta M, Conn KL, Lazarovits G, 2002. Volatile fatty acids in liquid swine manure can kill microsclerotia of Verticillium dahliae. Phytopathology 92: 548-552.
http://dx.doi.org/10.1094/PHYTO.2002.92.5.548
PMid:18943030

Van Bruggen AHC, Semenov AM, 2000. In search of biological indicators for soil health and disease suppression. Appl Soil Ecol 15: 13-24.
http://dx.doi.org/10.1016/S0929-1393(00)00068-8

Vance ED, Brookes PC, Jenkinson DS, 1987. An extraction method for measuring soil microbial biomass C. Soil Biol Biochem 19: 703-707.
http://dx.doi.org/10.1016/0038-0717(87)90052-6

Publicado
2012-06-27
Cómo citar
Nuñez-Zofío, M., Larregla del Palacio, S., & Garbisu, C. (2012). La reiteración de la biodesinfección controla la incidencia de la podredumbre radicular y del cuello del pimiento causada por Phytophthora y mejora la calidad del suelo. Spanish Journal of Agricultural Research, 10(3), 794-805. https://doi.org/10.5424/sjar/2012103-571-11
Sección
Protección vegetal