Survival percentage, photosynthetic abilities and growth characters of two indica rice (Oryza sativa L. spp. indica) cultivars in response to iso-osmotic stress
Abstract
The aim of this study was to investigate pigment degradation, chlorophyll fluorescence diminution, photosynthetic ability and growth reduction in two rice cultivars, in response to either iso-osmotic salt stress or water-deficit stress. Seedlings of rice cultivars RD6 and KDML105 were photo-autotrophically grown in MS media and subsequently exposed to –0.23 (control), –0.40 or –0.67 MPa iso-osmotic NaCl (salt stress) or mannitol (water-deficit stress) for 14 days. The survival percentage of the two rice cultivars reduced dramatically when subjected to –0.67 MPa NaCl treatment. Chlorophyll a (Chla), chlorophyll b (Chlb), total carotenoids (Cx+c), maximum quantum yield of PSII (Fv/Fm) and photon yield of PSII (ΦPSII) in the stressed seedlings were significantly lower when compared to seedlings in the control group (without mannitol or NaCl), leading to low net-photosynthetic rate (Pn) and growth reduction. In addition, the growth characters of plantlets in the salt stress conditions were more sharply reduced, and the physiological changes greater than those in water-deficit stress conditions. On the other hand, non-photochemical quenching in the leaves of stressed plantlets increased significantly, especially in response to iso-osmotic salt stress. In the present study, overall growth performance and physiological characters of KDML105 grown under iso-osmotic stress were better than those of RD6.
Downloads
References
Ahmad M.S.A., Javed F., Ashraf M., 2007. Iso-osmotic effect of NaCl and PEG on growth, cations and free proline accumulation in callus tissues of two indica rice (Oryza sativa L.) genotypes. Plant Growth Regul 53, 53–63. http://dx.doi.org/10.1007/s10725-007-9204-0
Ariyaphanphitak W., Chidthaisong A., Sarobol E., Bashkin V.N., Towprayoon S., 2005. Effects of elevated ozone concentrations on Thai jasmine rice cultivars (Oryza sativa L). Water Air Soil Poll 167, 179–200. http://dx.doi.org/10.1007/s11270-005-8650-4
Bahaji A., Mateu I., Sanz A., Cornejo M.J., 2002. Common and distinctive responses of rice seedlings to saline- and osmotically-generated stress. Plant Growth Regul 38, 83–94. http://dx.doi.org/10.1023/A:1020979621681
Castillo E.G., Tuong T.P., Ismail A.M., Inubushi K., 2007. Response to salinity in rice: Comparative effects of osmotic and ionic stresses. Plant Prod Sci 10, 159–170. http://dx.doi.org/10.1626/pps.10.159
Cha-Um S., Supaibulwatana K., Kirdmanee C., 2006. Water relation, photosynthetic ability, and growth of Thai jasmine rice (Oryza sativa L. ssp. indica cv. KDML105) to salt stress by application of exogenous glycinebetaine and choline. J Agron Crop Sci 192, 25–36. http://dx.doi.org/10.1111/j.1439-037X.2006.00186.x
Cha-Um S., Supaibulwatana K., Kirdmanee C., 2007a. Glycinebetaine accumulation, physiological characterizations, and growth efficiency in salt tolerant and salt sensitive lines of indica rice (Oryza sativa L. spp. indica) response to salt stress. J Agron Crop Sci 193, 157–166. http://dx.doi.org/10.1111/j.1439-037X.2007.00251.x
Cha-Um S., Vejchasarn P., Kirdmanee C., 2007b. An effective defensive response in Thai aromatic rice variwties (Oryza sativa L. spp. indica) to salinity. J Crop Sci. Biotechnol 10, 257–264.
Cha-Um S., Kirdmanee C., 2008. Assessment of salt tolerance in Eucalyptus, rain tree and Thai neem under laboratory and the field conditions. Pak J Bot 40, 2041–2051.
Cha-Um S., Nhung N.T.H., Kirdmanee C., 2010. Effect of mannitol- and salt-induced iso-osmotic stress on proline accumulation, photosynthetic abilities and growth characters of rice cultivars (Oryza sativa L. spp. indica). Pak J Bot 42, 927–941.
Dionisio-Sese M.L., Tobita S., 1998. Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135, 1–9. http://dx.doi.org/10.1016/S0168-9452(98)00025-9
Flowers T.J., Flowers S.A., 2005. Why dose salinity pose such a difficult problem for plant breeders? Agric Water Manage 78, 15–24. http://dx.doi.org/10.1016/j.agwat.2005.04.015
Fujiwara K., Kozai T., Watanabe I., 1987. Fundamental studies on environment in plant tissue culture vessels. (3) Measurements of carbon dioxide gas concentration in closed vessels containing tissue cultured plantlets and estimates of net-photosynthetic rates of the plantlets. J Agric Method 4, 21–30. http://dx.doi.org/10.2480/agrmet.43.21
Fukuda A., Nakamura A., Tagiri A., Tanaka H., Miyao A., Hirochika H., Tanaka Y., 2004. Function, intracellular localization, and the importance in salt tolerance of a vacuolar Na+/H+ antiporter from rice. Plant Cell Physiol. 45, 146–159. http://dx.doi.org/10.1093/pcp/pch014
GREGORIO G.B., SENADHIRA D., MENDOZA R.D., 1997. Screening rice for salinity tolerance. IRRI Discussion Paper Series No. 22, International Rice Research, pp. 1–34.
Gregorio G.B., Senadhira D., Mendoza R.D., Manigbas N.L., Roxas J.P., Guerta C.Q., 2002. Progress in breeding for salinity tolerance and associated abiotic stresses in rice. Field Crops Res 76, 91–101. http://dx.doi.org/10.1016/S0378-4290(02)00031-X
Hasegawa P.M., Bressan R.A., Zhu J.K., Bohnert H.J., 2000. Plant cellular and molecular responses to high salinity. Ann. Rev. Plant Physiol. Mol. Biol 51, 463–499. http://dx.doi.org/10.1146/annurev.arplant.51.1.463
Hien D.T., Jacobs M., Angenon G., Hermans C., Thu T.T., Van Son L., Roosens H., 2003. Proline accumulation and Δ1-pyrroline-5-carboxylate synthetase gene properties in three rice cultivars differing in salinity and drought tolerance. Plant Sci 165, 1059–1068. http://dx.doi.org/10.1016/S0168-9452(03)00301-7
Keeratipibul S., Luangsakul N., Lertsatchayarn T., 2008. The effect of Thai glutinous rice cultivars, grain length and cultivating locations on the quality of rice cracker (arare). LWT-Food Sci Technol 41, 1934–1943. http://dx.doi.org/10.1016/j.lwt.2007.12.008
Khan M.A., Abdullah Z., 2003. Salinity-sodicity induced changes in reproductive physiology of rice (Oryza sativa) under dense soil conditions. Environ Exp Bot 49, 145–157. http://dx.doi.org/10.1016/S0098-8472(02)00066-7
Khush G.S., 2005. What it will take to feed 5.0 billion rice consumers in 2030. Plant Mol Biol 59, 1–6. http://dx.doi.org/10.1007/s11103-005-2159-5
Kirdmanee C., Mosaleeyanon K., 2000. Environmental engineering for transplant production. In Kubota C., Chun C., Transplant Production in the 21st Century. Kluwer Academic Publishers, Netherlands. pp. 78–81. http://dx.doi.org/10.1007/978-94-015-9371-7_12
Laohakunjit N., Kerdchoechuen O., 2007. Aroma enrichment and the change during storage of non-aromatic milled rice coated with extracted natural flavor. Food Chem 101, 339–344. http://dx.doi.org/10.1016/j.foodchem.2005.12.055
Lee I.S., Kim D.S., Kang S.Y., Wi S.G., Jin H., Yun P.Y., Lim Y.P., Lee Y.I., 2004. Characterizing salt stress response in a rice variety and its salt tolerant lines derived from in vitro mutagenesis. J Plant Biotechnol 6, 205–212.
Lichtenthaler H.K., 1987. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods Enzymol 148, 350–380. http://dx.doi.org/10.1016/0076-6879(87)48036-1
Loggini B., Scartazza A., Brugnoli E., Navari-Izzo F., 1999. Antioxidant defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiol 119, 1091–1099. http://dx.doi.org/10.1104/pp.119.3.1091
Luo Q., Yu B., Liu Y., 2005. Differential sensitivity to chloride and sodium ions in seedlings of Glycine max and G. soja under NaCl stress. J Plant Physiol 162, 1003-1012. http://dx.doi.org/10.1016/j.jplph.2004.11.008
Lutts S., Almansouri M., Kinet J.M., 2004. Salinity and water stress have contrasting effects on the relationship between growth and cell viability during and after stress exposure in durum wheat callus. Plant Sci 167, 9–18. http://dx.doi.org/10.1016/j.plantsci.2004.02.014
Mahajan S., Tuteja N., 2005. Cold, salinity and drought stresses: An overview. Arch Biochem Biophys 444, 139–158. http://dx.doi.org/10.1016/j.abb.2005.10.018
Martinez-Atienza J., Jiang X., Garciadeblas B., Mendoza I., Zhu J.K., Pardo J.M., Quintero F.J., 2007. Conservation of the salt overlay sensitive pathway in rice. Plant Physiol 143, 1001–1012. http://dx.doi.org/10.1104/pp.106.092635
Maxwell K., Johnson G.N., 2000. Chlorophyll fluorescence – a practical guide. J Exp Bot 51, 659–668. http://dx.doi.org/10.1093/jexbot/51.345.659
Morsy M.R., Jouve L., Hausman J.F., Hoffmann L., Steward J.M., 2007. Alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice (Oryza sativa L.) genotypes contrasting in chilling tolerance. J Plant Physiol 164, 157–167. http://dx.doi.org/10.1016/j.jplph.2005.12.004
Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15, 473–479. http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
Parida A.K., Das A.B., 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicol Environ Safe 60, 324–349. http://dx.doi.org/10.1016/j.ecoenv.2004.06.010
Reddy A.R., Chitanya K.V., Vivekanandan M., 2004. Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol 161, 1189–1202. http://dx.doi.org/10.1016/j.jplph.2004.01.013
Sairam R.K., Tyagi A., 2004. Physiology and molecular biology of salinity stress tolerance in plants. Curr Sci 86, 407–421.
Senadhira D., Zapata-Arias F.J., Gregorio G.B., Alejar M.S., De La Cruz H.C., Padolina T.F., Galvez A.M., 2002. Development of the first salt-tolerant rice cultivar through indica/indica anther culture. Field Crops Res 76, 103–110. http://dx.doi.org/10.1016/S0378-4290(02)00032-1
Shabala S.N., Shabala S.I., Martynenko A.I., Babourina O., Newman I.A., 1998. Salinity effect on bioelectric activity, growth, Na+ accumulation and chlorophyll fluorescence of maize leaves: a comparative survey and prospects for screening. Aust J Plant Physiol 25, 609–616. http://dx.doi.org/10.1071/PP97146
Shannon M.C., Rhoades J.D., Draper J.H., Scardaci S.C., Spyres M.D., 1998. Assessment of salt tolerance in rice cultivars in response to salinity problems in California. Crop Sci 38, 394–398. http://dx.doi.org/10.2135/cropsci1998.0011183X003800020021x
Shereen A., Ansari R., Mumtaz S., Bughio H.R., Mujtaba S.M., Shirazi M.U., Khan M.A., 2009. Impact of gamma irradiation induced changes on growth and physiological responses of rice under saline conditions. Pak J Bot 41, 2487-2495.
Sultana N., Ikeda T., Itoh R., 1999. Effect of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grains. Environ Exp Bot 42, 211–220. http://dx.doi.org/10.1016/S0098-8472(99)00035-0
Uddin M.I., Rashid M.H., Khan N., Perveen M.F., Tai T.H., Tanaka K., 2007. Selection of promising salt tolerant rice mutants derived from cultivar 'Drew' and their antioxidant enzymes activity under salt stress. SABRAO J Breed Genet 39, 89–98.
Vaidynathan H., Sivakumar P., Chakrabarty R., Thomas G., 2003. Scavenging of reactive oxygen species in NaCl-stressed rice (Oryza sativa L.) –differential response in salt-tolerant and sensitive varieties. Plant Sci 165, 1411–1418. http://dx.doi.org/10.1016/j.plantsci.2003.08.005
Wahid A., 2004. Analysis of toxic and osmotic effects of sodium chloride on leaf growth and economic yield of sugarcane. Bot Bull Acad Sin 45, 133–141.
Wang W., Vinocur B., Shoseyov O., Altman A., 2001. Biotechnology of plant osmotic stress tolerance: physiological and molecular considerations. Acta Hort 560, 285–292. http://dx.doi.org/10.17660/ActaHortic.2001.560.54
Wang W., Vinocur B., Altman A., 2003. Plant responses to drought, salinity and extreme temperature: towards genetic engineering for stress tolerance. Planta 218, 1–14. http://dx.doi.org/10.1007/s00425-003-1105-5
Wongpokkhom N., Kheoruenromne I., Suddhiprakarn A., Gilkes R.J., 2008. Micromorphological properties of salt affected soils in Northeast Thailand. Geoderma 144, 158–170. http://dx.doi.org/10.1016/j.geoderma.2007.10.026
Zang X., Komatsu S., 2007. A proteomics approach for identifying osmotic-stress-related proteins in rice. Phytochem 68, 426–437. http://dx.doi.org/10.1016/j.phytochem.2006.11.005
Zeng L., Shannon M.C., 2000. Salinity effects on seedling growth and yield components of rice. Crop Sci 40, 996–1003. http://dx.doi.org/10.2135/cropsci2000.404996x
Zeng L., Lesch S.M., Grieve C.M., 2003. Rice growth and yield respond to changes in water depth and salinity stress. Agric Water Manage 59, 67–75. http://dx.doi.org/10.1016/S0378-3774(02)00088-4
© CSIC. Manuscripts published in both the print and online versions of this journal are the property of the Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.
All contents of this electronic edition, except where otherwise noted, are distributed under a Creative Commons Attribution 4.0 International (CC BY 4.0) licence. You may read the basic information and the legal text of the licence. The indication of the CC BY 4.0 licence must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the final version of the work produced by the publisher, is not allowed.
