Short communication. Comparison of the efficacy of Apiguard (thymol) and Apivar (amitraz) in the control of Varroa destructor (Acari: Varroidae)
Abstract
The present study compared the efficacy of Apivar (a.i. amitraz) and Apiguard (a.i. thymol) in controlling the mite Varroa destructor during spring 2010 and autumn 2011, in the Balearic Islands (Spain). Number of fallen mites (NFM) was counted weekly and the efficacy of treatments was evaluated by using the percentage of reduction of the average daily fallen mites (%R). During spring assay, the average NFM was highly reduced in Apiguard (89.8%) compared to Apivar (64.3%) group, with significant differences between Apiguard and control group (untreated group) in post-treatment week. In autumn assay, Apivar and Apiguard colonies had an average reduction of the NFM of 17.9% and 30.8% respectively, showing a tendency in reduction between control and Apiguard group in post-treatment week. In both assays, %R was higher in Apiguard than in Apivar, but no significant differences were found between treatments in any of the seasons. Apiguard was less efficacious during November-December, probably due to the low external temperatures that hampered an optimal volatilization of the product. The lower efficacy of Apivar is probably related to the resistance of V. destructor to this chemical miticide, which has been used during the last 30 years. Results of this study showed that in Mediterranean conditions, spring is an appropriate period for applying Apiguard to the colonies, whereas application in late autumn would decrease the efficacy of the product. Apiguard may represent an alternative product for integrated control due to the low risk of mite resistance and residues in bee products.
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References
Akyol E, Yeninar H, 2008. Controlling Varroa destructor (Acari: Varroidae) in honeybee Apis mellifera (Hymenoptera: Apidae) colonies by using Thymovar (R) and BeeVital (R). Ital J Anim Sci 7(2): 237-242.
Anderson D, Trueman J, 2000. Varroa jacobsoni (Acari: Varroidae) is more than one species. Exp Appl Acarol 24(3): 165-189. http://dx.doi.org/10.1023/A:1006456720416
Boecking O, Genersch E, 2008. Varroosis - The ongoing crisis in bee keeping RID C-2232-2009. J Fur Verbrauch Lebens 3(2): 221-228. http://dx.doi.org/10.1007/s00003-008-0331-y
Bogdanov S, 2006. Contaminants of bee products. Apidologie 37: 1-18. http://dx.doi.org/10.1051/apido:2005043
Chauzat M, Carpentier P, Martel A, Bougeard S, Cougoule N, Porta P, Lachaize J, Madec F, Aubert M, Faucon J, 2009. Influence of pesticide residues on honey bee (Hymenoptera: Apidae) colony health in France. Environ Entomol 38(3): 514-523. http://dx.doi.org/10.1603/022.038.0302
Chiesa F, 1991. Effective control of varroatosis using powdered thymol. Apidologie 22(2): 135-145. http://dx.doi.org/10.1051/apido:19910206
Chuda-Mickiewicz B, Prabucki J, Jerzy PR, 2007. Evaluation of varroacidal efficacy of biowar preparation. J Apic Sci 51 (2): 47-53.
Colombo M, Spreafico M, 1999. Esperienze di lotta a Varroa jacobsoni Oud. con un nuovo formulato a base di timolo. La Selezione Veteterinaria 7: 473-478.
Dietemann V, Nazzi F, Martin SJ, Anderson D, Locke B, Delaplane KS, Wauquiez Q, Tannahill C, Frey E, Ziegelmann B, Rosenkranz P, Ellis JD, 2013. Standard methods for varroa research: a review. In: The coloss beebook, Volume II: Standard methods or Apis mellifera pest and pathogen research; Dietemann V, Ellis JD, Neumann P (eds). J Apic Res 52(1): 1-54. http://dx.doi.org/10.3896/ibra.1.52.1.09
Ellis JD, 2001. The future of Varroa control: Integrating current treatments with the latest advancements. Am Bee J 141(2): 127-131.
Elzen PJ, Baxter, JR, Spivak M, Wilson WT, 1999. Amitraz resistance in varroa: New discovery in North America. Am Bee J 139(5): 362.
Faucon J, Drajnudel P, Chauzat MP, Aubert M, 2007. Control of the efficacy of APIVAR ND against Varroa destructor, a parasite of Apis mellifera. Rev Med Vet-Toulouse 158(6): 283-290.
Floris I, Satta A, Garau VL, Melis M, Cabras P, Aloul N, 2001. Effectiveness, persistence, and residue of amitraz plastic strips in the apiary control of Varroa destructor. Apidologie 32(6): 577-585. http://dx.doi.org/10.1051/apido:2001145
Floris I, Satta A, Cabras P, Garau VL, Angioni A, 2004. Comparison between two thymol formulations in the control of Varroa destructor: Effectiveness, persistence, and residues. J Econ Entomol 97(2): 187-191. http://dx.doi.org/10.1093/jee/97.2.187
Fries I, Aarhus A, Hansen H, Korpela S, 1991. Comparison of diagnostic methods for detection of low infestation levels of Varroa jacobsoni in honeybee (Apis mellifera) colonies. Exp Appl Acarol 10 (3-4): 279-287. http://dx.doi.org/10.1007/BF01198656
Gashout HA, Guzman-Novoa E, 2009. Acute toxicity of essential oils and other natural compounds to the parasitic mite, Varroa destructor, and to larval and adult worker honey bees (Apis mellifera L.). J Apicult Res 48(4): 263-269.
Gregorc A, Planinc I, 2005. The control of Varroa destructor in honey bee colonies using the thymol-based acaricide - Apiguard. Am Bee J 145(8): 672-675.
Henderson C, Tilton E, 1955. Tests with acaricides against the brown wheat mite. J Econ Entomol 48(2): 157-161. http://dx.doi.org/10.1093/jee/48.2.157
Hollingworth RM, 1976. Chemistry, biological-activity, and uses of formamidine pesticides. Environ Health Persp 14: 57-69. http://dx.doi.org/10.1289/ehp.761457
Imdorf A, Bogdanov S, Kilchenmann V, Maquelin C, 1995. Apilife VAR - A new varroacide with thymol as the main ingredient. Bee World 76(2): 77-83. http://dx.doi.org/10.1080/0005772X.1995.11099245
Johnson RM, Pollock HS, Berenbaum MR, 2009. Synergistic interactions between in-colony miticides in Apis mellifera. J Econ Entomol 102(2): 474-479. http://dx.doi.org/10.1603/029.102.0202
Lodesani M, Costa C, Serra G, Colombo R, Sabatini AG, 2008. Acaricide residues in bees wax after conversion to organic beekeeping methods. Apidologie 39(3): 324-333. http://dx.doi.org/10.1051/apido:2008012
Loucif-ayad W, Aribi N, Smagghe G, Soltani N, 2010. Comparative effectiveness of some acaricides used to control Varroa destructor (Mesostigmata: Varroidae) in Algeria. Afr Entomol 18(2): 259-266. http://dx.doi.org/10.4001/003.018.0211
Marchetti S, Barbattini R, 1984. Comparative effectiveness of treatments used to control Varroa jacobsoni Oud. Apidologie 15(4): 363-377. http://dx.doi.org/10.1051/apido:19840401
Martel A, Zeggane S, Aurieres C, Drajnudel P, Faucon J, Aubert M, 2007. Acaricide residues in honey and wax after treatment of honey bee colonies with Apivar (R) or Asuntol (R) 50. Apidologie 38(6): 534-544. http://dx.doi.org/10.1051/apido:2007038
Pohorecka K, Bober A, Skubida M, Zdanska D, 2011. Epizootic status of apiaries with massive losses of bee colonies (2008-2009). J Apic Sci 55 (1): 137-150.
Rosenkranz P, Aumeier P, Ziegelmann B, 2010. Biology and control of Varroa destructor. J Invertebr Pathol 103: S96-S119. http://dx.doi.org/10.1016/j.jip.2009.07.016
Satta A, Floris I, Eguaras M, Cabras P, Garau V, Melis M, 2005. Formic acid-based treatments for control of Varroa destructor in a Mediterranean area. J Econ Entomol 98(2): 267-273. http://dx.doi.org/10.1093/jee/98.2.267
Trouiller J, 1998. Monitoring Varroa jacobsoni resistance to pyrethroids in western Europe. Apidologie 29(6): 537-546. http://dx.doi.org/10.1051/apido:19980606
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