Genetic structure of Spanish populations of Ceratitis capitata revealed by RAPD and ISSR markers: implications for resistance management
Abstract
The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is considered one of the most economically damaging pests of citrus orchards in Spain. The characterization of C. capitata population structure, at a large geographical scale, by using a combination of RAPD and ISSR markers can allow analyzing the genetic variability of this species, and provide some insight in decision making for resistance management, recently recorded in Spain. We compared six Spanish populations along the Mediterranean area (Gerona, Amposta, Tortosa, Castellón, Valencia and Málaga) with populations from other geographical areas where this pest is widely distributed (Africa, Middle East, South America and Atlantic Islands) and two laboratory strains. The results obtained with both types of molecular markers were similar. A dendrogram based on Nei genetic distances showed that all Mediterranean Spanish populations, except the population collected in Gerona, were clearly separated from the rest. However, no clear differentiation among Spanish populations was found, probably as a result of the high levels of gene flow (Nm value of 2.8 for RAPD and 3.9 for ISSR). Implications of these findings on resistance management of C. capitata are discussed.Downloads
References
Alaoui A, Imoulan A, El Alaoui Z, El Meziane A, 2010. Genetic structure of Mediterranean fruit fly (Ceratitis capitata) populations from Moroccan endemic forest of Argania spinosa. Int J Agri Biol 12: 291-298.
Aubert H, Lightner DV, 2000. Identification of genetic populations of the Pacific blue shrimp Penaeus stylirostris of the Gulf of California, Mexico. Mar Biol 137: 875-885.
http://dx.doi.org/10.1007/s002270000419
Barr NB, 2009. Pathway analysis of Ceratitis capitata (Diptera: Tephritidae) using mitochondrial DNA. J Econ Entomol 102(1): 401-411.
http://dx.doi.org/10.1603/029.102.0153
PMid:19253662
Baruffi L, Damiani G, Guglielmino CR, Bandi C, Malacrida AR, Gasperi, G, 1995. Polymorphism within and between populations of Ceratitis capitata: comparison between RAPD and multilocus enzyme electrophoresis data. Heredity 74: 425-437.
http://dx.doi.org/10.1038/hdy.1995.60
PMid:7759289
Black IV WC, 1995. Fortran programs for the analysis of RAPD-PCR markers in populations. Colorado St Univ Ft. Collins, CO, USA.
Couso-Ferrer F, Arouri R, Beroiz B, Perera N, Cervera A, Navarro-Llopis V, Castaera P, Hernndez-Crespo P, Ortego F, 2011. Cross-resistance to insecticides in a malathion-resistant strain of Ceratitis capitata (Diptera: Tephritidae). J Econ Entomol 104(4): 1349-1356.
http://dx.doi.org/10.1603/EC11082
PMid:21882703
De Breme F, 1842. Note sur le genre Ceratitis de M. Mac heay (Diptera). Ann Soc Entomol Fr 11: 183-190.
De Meyer M, Robertson MP, Peterson AT, Mansell, MW, 2008. Ecological niches and potential geographical distributions of Mediterranean fruit fly (Ceratitis capitata) and Natal fruit fly (Ceratitis rosa). J Biogeogr 35(2): 270-281.
Excoffier L, Smouse PE, Quattro JM, 1992. Analysis of molecular variance from metric distance among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131: 479-491.
PMid:1644282 PMCid:1205020
Fernndez P, 2002. Estudios gentico-poblacionales en Ceratitis capitata Wied. Doctoral Thesis. Univ. Complutense de Madrid, Madrid, Spain.
Fimiani P, 1989. Mediterranean region. In: Fruit flies: their biology, natural enemies and control, vol 3A (Robinson AS and Hooper G, eds). Elsevier, Amsterdam, Netherlands. pp: 37-50.
Gomulski L, Bourtzis K, Brogna S, Morandi PA, Bonvicini C, Sebastian F, Torti C, Guglielmino CR, Savakis C, Gasperi G, Malacrida AR, 1998. Intron size polymorphism of the Adh1 gene parallels the worldwide colonization history of the Mediterranean fruit fly, Ceratitis capitata. Mol Ecol 7: 1729-1741.
http://dx.doi.org/10.1046/j.1365-294x.1998.00509.x
Haymer DS, McInnis DO, 1994. Resolution of populations of the Mediterranean fuit fly at the DNA level using random primers for the polymerase chain reaction. Genome 37: 244-248.
http://dx.doi.org/10.1139/g94-034
PMid:8200514
He M, Haymer DS, 1999. Genetic relationships of populations and the origins of new infestations of the Mediterranean fruit fly. Mol Ecol 8: 1247-1257.
http://dx.doi.org/10.1046/j.1365-294X.1999.00685.x
PMid:10447866
Jain SK, Neekhra B, Pandey D, Jain K, 2010. RAPD marker system in insect study: a review. Indian J Biotechnol 9: 7-12.
Lynch M, Milligan BG, 1994. Analysis of population genetic structure with RAPD markers. Mol Ecol 3: 91-99.
http://dx.doi.org/10.1111/j.1365-294X.1994.tb00109.x
PMid:8019690
Magaa C, Hernndez-Crespo P, Ortego F, Castaera P, 2007. Resistance to malathion in field populations of Ceratitis capitata. J Econ Entomol 100: 1836-1843.
http://dx.doi.org/10.1603/0022-0493(2007)100[1836:RTMIFP]2.0.CO;2
Magaa C, Hernndez-Crespo P, Brun-Barale A, Couso-Ferrer F, Bride J-M, Castaera P, Feyereisen R, Ortego F, 2008. Mechanisms of resistance to malathion in the medfly Ceratitis capitata. Insect Biochem Molec 38(8): 756-762.
http://dx.doi.org/10.1016/j.ibmb.2008.05.001
PMid:18625398
Malacrida AR, Marinoni F, Torti C, Gomulski LM, Sebastiani F, Bonuicini C, Gasperi G, Guglielmino CR, 1998. Genetic aspects of the worldwide colonization process of Ceratitis capitata. J Hered 89: 501-507.
http://dx.doi.org/10.1093/jhered/89.6.501
PMid:9864861
Martins-Lopes P, Lima-Brito J, Gomes S, Meirinhos J, Santos L, Guedes-Pinto H, 2007. RAPD and ISSR molecular markers in Olea europaea L.: variability and molecular cultivar identification. Genet Resour Crop Ev 54: 117-128.
http://dx.doi.org/10.1007/s10722-005-2640-7
Nei M, 1972. Genetic distances between populations. Am Nat 106: 283-292.
http://dx.doi.org/10.1086/282771
Nei M, Li WH, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. P Natl Acad Sci USA 76: 52695273.
http://dx.doi.org/10.1073/pnas.76.10.5269
Ochando MD, Reyes A, Callejas C, Segura D, Fernndez P, 2003. Molecular genetic methodologies applied to the study of fly pests. Trends Entomol 3: 7385.
Ochando MD, Rodrguez S, Hernndez S, Callejas C, 2010. The ISSR useful molecular tools for olive fly studies. Integrated Protection of Olive Crops IOBC/wprs Bulletin 59: 11-19.
Powell W, Morgante M, Andre C, Hanafey M, 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germoplasm analysis. Mol Breeding 2: 225.238.
Raina, S N, Rani V, Kojima T, Ogihara Y, Singh KP, Devarumath RM, 2001. RAPD and ISSR fingerprints as useful genetic markers for analysis of genetic diversity, varietal identification, and phylogenetic relationships in peanut (Arachis hypogaea) cultivars and wild species. Genome 44: 763-772.
PMid:11681599
Reyes A, Ochando MD, 1994. A study of gene-enzyme variability in three Spanish populations of Ceratitis capitata. IOBC/wprs Bulletin 20: 151160.
Reyes A, Ochando MD, 1998. Use of molecular markers for detecting the geographical origin of Ceratitis capitata (Diptera: Tephritidae) populations. Ann Entomol Soc Am 91 (2): 222-227.
Reyes A, Ochando MD, 2004. Mitochondrial DNA variation in Spanish populations of Ceratitis capitata (Wiedemann) (Tephritidae) and the colonization process. J Appl Entomol 128 (5): 358-364.
http://dx.doi.org/10.1111/j.1439-0418.2004.00858
Rohlf J, 1997. Numerical taxonomy and multivariate analysis system NTSYS-pc v2.01b. Dept Ecology and Evolution, State University of New York, NY, USA.
Schneider S, Roessli D, Excoffier L, 2000. Arlequin: a software for population genetics data analysis, vers 2000. Genetics and Biometry Lab, Dept Anthropology and Ecology. Univ Geneva, Switzerland.
Solano P, Ravel S, Bouyer J, Camara M, Kagbadouno MS, Dyer N, Gardes L, Herault D, Donnelly MJ, De Mees T, 2009. The population structure of Glossina palpalis gambiensis from island and continental locations in coastal Guinea. PLoS Negl. Trop Dis 3(3): e392.
http://dx.doi.org/10.1371/journal.pntd.0000392
PMid:19290038 PMCid:2652410
Soliani C, Rondan-Dueas J, Chiappero MB, Martnez M, Garca E, Garcenal CN, 2010. Genetic relationships among populations of Aedes aegypti from Uruguay and northeastern Argentina inferred from ISSR-PCR. Med Vet Entomol 24: 316323.
PMid:20626627
SPSS, 2009. SPSS 17.0. SPSS Inc., Chicago, IL, USA.
Viuela E, 1998. La resistencia a insecticidas en Espaa. Bol San Veg Plagas 24: 487-496.
Weeks AR, Endersby NM, Lange CL, Lowe A, Zalucki MP, Hoffmann AA, 2010. Genetic variation among Helicoverpa armigera populations as assessed by microsatellites: a cautionary tale about accurate allele scoring. B Entomol Res 100: 445450.
http://dx.doi.org/10.1017/S0007485309990460
PMid:20003572
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531-6535.
http://dx.doi.org/10.1093/nar/18.22.6531
PMid:1979162 PMCid:332606
Wright S, 1951. The genetical structure of populations. Ann Eugenics 15: 323-354.
http://dx.doi.org/10.1111/j.1469-1809.1949.tb02451.x
Zietkiewicz E, Rafalski A, Labuda D, 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176-183.
http://dx.doi.org/10.1006/geno.1994.1151
PMid:8020964
© CSIC. Manuscripts published in both the printed and online versions of this Journal are the property of Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.
All contents of this electronic edition, except where otherwise noted, are distributed under a “Creative Commons Attribution 4.0 International” (CC BY 4.0) License. You may read here the basic information and the legal text of the license. The indication of the CC BY 4.0 License must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the published by the Editor, is not allowed.
