Short communication: A potential role of tannins in the control of American foulbrood
Abstract
Aim of study: To evaluate the use of tannins extracts in the control of the American foulbrood pathology and to investigate if these extracts present levels of toxicity on Apis mellifera.
Area of study: Paenibacillus larvae strains C1 and C2 were from Balcarce, province of Buenos Aires, strain C6 from Rio Cuarto, in Cordoba province and strain C9 from Concordia in Entre Rios province. Bees larvae used for toxicological assays were collected in Santa Paula experimental apiary, Mar del Plata (belonging to the Centro de Investigación en Abejas Sociales (CIAS-IIPROSAM) from UNMdP.
Material and methods: The minimal inhibitory concentration (MIC) of five different tannin extracts were obtained by agar diffusion method on four P. larvae strains; using the MIC value, the toxicity test on A. mellifera larvae was performed afterwards.
Main results: The MIC value was in the range of 6.9 to 898.6 µg/mL. Three tannin extracts did not show toxicity against bee larvae; however, those that were fed with the latter showed a significant increase in weight.
Research highlights: Three tanninS extracts showed a good antimicrobial activity against P. larvae and they did not show toxicity against bee larvae.
Downloads
References
Aupinel P, Fortini D, Dufour H, Tasei J, Michaud B, Odoux J, Pham-Delegue M, 2005. Improvement of artificial feeding in a standard in vitro method for rearing Apis mellifera larvae. Bull Insectology 58:107-111.
Bernklau E, Bjostad L, Hogeboom A, Carlisle A, 2019. Dietary phytochemicals, honey bee longevity and pathogen tolerance. Insects 10: 14-26. https://doi.org/10.3390/insects10010014
Bonev B, Hooper J, Parisot J, 2008. Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method. J Antimicrob Chemother 61: 1295-1301. https://doi.org/10.1093/jac/dkn090
Chandra H, Bishnoi P, Yadav A, Patni B, Mishra A, Nautiyal A, 2017. Antimicrobial resistance and the alternative resources with special emphasis on plant-based antimicrobials - A review. Plants 6: 16-27. https://doi.org/10.3390/plants6020016
Duarte MCT, Leme EE, Delarmelina C, Soares AA, Figueira GM, Sartoratto A, 2007. Activity of essential oils from Brazilian medicinal plants on Escherichia coli. J Ethnopharmacol 111: 197-201. https://doi.org/10.1016/j.jep.2006.11.034
Evans J, Lopez D, 2004. Bacterial probiotics induce an immune response in the honey bee (Hymenoptera: Apidae). J Econ Entomol 97: 752-756. https://doi.org/10.1093/jee/97.3.752
Genersch E, 2010. American Foulbrood in honeybees and its causative agent, Paenibacillus larvae. J Iinvertebr Pathol 103: S10-S19. https://doi.org/10.1016/j.jip.2009.06.015
Genersch E, Forsgren E, Pentikäinen J, Ashiralieva A, Rauch S, Kilwinski J, Fries I, 2006. Reclassification of Paenibacillus larvae subsp. pulvifaciens and Paenibacillus larvae subsp. larvae as Paenibacillus larvae without subspecies differentiation. Int J Syst Evol Microbiol 56: 501-511. https://doi.org/10.1099/ijs.0.63928-0
Giménez-Martínez P, Cugnata N, Alonso-Salces RM, Arredondo D, Antunez K, De Castro R, Fuselli SR, 2019. Short communication: Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.). Span J Agric Res 17 (3): e05SC01 https://doi.org/10.5424/sjar/2019173-14740
Hansen H, Brødsgaard C, 1999. American foulbrood: a review of its biology, diagnosis and control. Bee World 80: 5-23. https://doi.org/10.1080/0005772X.1999.11099415
Isaza JH, 2007. Taninos o polifenoles vegetales. Scientia et technica 1: 13-18.
Iwu MW, Duncan AR, Okunji CO, 1999. New antimicrobials of plant origin. In: Perspectives on new crops and new uses; J. Janick (Ed). pp: 457-462. ASHS Press, Alexandria.
Li N, Tong X, Zeng J, Meng G, Sun F, Hu H, Song J, Lu C, Dai F, 2019. Hippo pathway regulates somatic development and cell proliferation of silkworm. Genomics 111: 391-397. https://doi.org/10.1016/j.ygeno.2018.02.014
Liu XL, Hao YQ, Jin L, Xu ZJ, McAllister TA, Wang Y, 2013. Anti-Escherichia coli O157:H7 properties of purple prairie clover and sainfoin condensed tannins. Molecules 18: 2183-2199. https://doi.org/10.3390/molecules18022183
Mao W, Schuler MA, Berenbaum MR, 2015. A dietary phytochemical alters caste-associated gene expression in honey bees. Sci Adv 1: e1500795. https://doi.org/10.1126/sciadv.1500795
McAllister TA, Martinez T, Bae HD, Muir AD, Yanke LJ, Jones GA, 2005. Characterization of condensed tannins purified from legume forages: chromophore production, protein precipitation, and inhibitory effects on cellulose digestion. J Chem Ecol 31: 2049-2068. https://doi.org/10.1007/s10886-005-6077-4
Michielin EM, Salvador AA, Riehl CA, Smânia Jr A, Smânia EF, Ferreira SR, 2009. Chemical composition and antibacterial activity of Cordia verbenacea extracts obtained by different methods. Bioresour Technol 100: 6615-6623. https://doi.org/10.1016/j.biortech.2009.07.061
Miyagi T, Peng C, Chuang R, Mussen E, Spivak M, Doi R, 2000. Verification of oxytetracycline-resistant American foulbrood pathogen Paenibacillus larvae in the United States. J Invertebr Pathol 75: 95-96. https://doi.org/10.1006/jipa.1999.4888
Nordström S, Fries I, 1995. A comparison of media and cultural conditions for identification of Bacillus larvae in honey. J Apic Res 34: 97-103. https://doi.org/10.1080/00218839.1995.11100894
OECD, 2013. Test No. 237: Honey bee (Apis Mellifera) larval toxicity test, single exposure. OECD Guidelines for the Testing of Chemicals, Sect. 2, OECD Publ, Paris. https://doi.org/10.1787/9789264203723-en
Schneck L, 2004. How a hippo came to work in an Alaska medical practice. An innovative approach to HIPAA compliance. MGMA Connexion 4: 33-34.
Sieniawska E, Baj T, 2017. Tannins. In: Pharmacognosy; Badal S & Delgoda R (Eds.). pp. 199-232. Academic Press, Boston. https://doi.org/10.1016/B978-0-12-802104-0.00010-X
Smith AH, Mackie RI, 2004. Effect of condensed tannins on bacterial diversity and metabolic activity in the rat gastrointestinal tract. Appl Environ Microbiol 70: 1104-1115. https://doi.org/10.1128/AEM.70.2.1104-1115.2004
Tang F, Zhang X, Liu Y, Gao X, Liu N, 2014. In vitro inhibition of glutathione S-transferases by several insecticides and allelochemicals in two moth species. Int J Pest Manag 60: 33-38. https://doi.org/10.1080/09670874.2014.894216
Wang YS, He HP, Yang JH, Di YT, Hao XJ, 2008. New monoterpenoid coumarins from Clausena anisum-olens. Molecules 13: 931-937. https://doi.org/10.3390/molecules13040931
Yuan Y, Li L, Zhao J, Chen M, 2020. Effect of tannic acid on nutrition and activities of detoxification enzymes and acetylcholinesterase of the fall webworm (Lepidoptera: Arctiidae). J Insect Sci 20: 1-7. https://doi.org/10.1093/jisesa/ieaa001
© CSIC. Manuscripts published in both the printed and online versions of this Journal are the property of Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.
All contents of this electronic edition, except where otherwise noted, are distributed under a “Creative Commons Attribution 4.0 International” (CC BY 4.0) License. You may read here the basic information and the legal text of the license. The indication of the CC BY 4.0 License must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the published by the Editor, is not allowed.
