Effects of dietary supplementation with garlic powder (Allium sativum L.) on broiler performance, carcass traits, lymphoid organ development and intestinal biometrics
Abstract
Aim of study: To assess the effects of dietary supplementation with garlic (Allium sativum L.) powder as a growth-promoting additive on the productive performance, carcass traits, intestinal biometrics, and lymphoid organ development of broilers.
Area of study: Machado, Minas Gerais, southeast region of Brazil.
Material and methods: A total of 660 one-day-old chicks were used in a completely randomized design with 5 treatments (0, 2.5, 5.0, 7.5, and 10.0 g/kg garlic powder), 6 replications, and 22 animals per experimental unit during 42 days of production, divided into phases: initial (1 to 7 days), growth (8 to 22 days), and finishing (23 to 42 days).
Main results: In the total production period, the birds that were fed rations containing 5, 7.5 and 10 g/kg garlic powder showed increased weight gain and feed conversion ratio (p<0.05). Among the carcass yield variables, live weight and carcass yield showed a positive linear effect (p<0.05). Birds supplemented with garlic powder showed increased weight (p<0.05) and relative length of the small intestine (p<0.05). The absolute weight of the lymphoid organs, thymus and bursa of Fabricius, and the relative weight of the thymus, bursa of Fabricius and spleen also increased linearly with the level of dietary supplementation with garlic powder (p<0.05).
Research highlights: Dietary supplementation with 5 g/kg and higher concentrations of garlic powder is recommended as a growth promoter for broilers because this feed additive enhances lymphoid organ development, improves intestinal biometric variables and consequently maximises the productive performance and carcass yield of broilers from 1 to 42 days of age.
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References
Abd El-Ghany WA, Shaalan M, Salem HM, 2021. Nanoparticles applications in poultry production: an updated review. Worlds Poult Sci J 77(4): 1001-1025. https://doi.org/10.1080/00439339.2021.1960235
Abd El-Hack AM, El-Saadony MT, Elbestawy AR, Gado AR, Nader MM, Saad AM, et al., 2022. Hot red pepper powder as a safe alternative to antibiotics in organic poultry feed: An updated review. Poult Sci 101(4): 1-13. https://doi.org/10.1016/j.psj.2021.101684
Adebiyi FG, Ologhobo AD, Adejumo IO, 2017. Modulation of cholesterol in laying chickens fed sun-dried garlic powder. J Exp Agric Int 19(2): 1-7. https://doi.org/10.9734/JEAI/2017/38168
Al-Massad M, Ramamneh DAL, Sharafat AA, Hussain N, 2018. Effect of using garlic on the economical and physiological characteristics of broiler chickens. Int J Environ Sci Nat Res 10(2): 1-5.
Alagawany M, Madkour M, El-Saadony MT, Reda FM, 2021a. Paenibacillus polymyxa (LM31) as a new feed additive: Antioxidant and antimicrobial activity and its effects on growth, blood biochemistry, and intestinal bacterial populations of growing Japanese quail. Anim Feed Sci Technol 276: 1-13. https://doi.org/10.1016/j.anifeedsci.2021.114920
Alagawany M, El-Saadony MT, Elnesr SS, Farahat M, Attia G, Madkour M, et al., 2021b. Use of lemongrass essential oil as a feed additive in quail's nutrition: its effect on growth, carcass, blood biochemistry, antioxidant and immunological indices, digestive enzymes and intestinal microbiota. Poult Sci 100(6): 1-8. https://doi.org/10.1016/j.psj.2021.101172
Alkassar AM, Alaboudy BAAS, 2021. Effect of added different levels of Graviola Leavs meal (Annona muricata L) on biochemical blood traits and bacteriological account in broilers chicken. IOP Conf Ser: Earth Environ Sci 735(1): 1-8. https://doi.org/10.1088/1755-1315/735/1/012023
Awad WA, Ghareeb K, Abdel-Raheem S, Böhm J. 2009. Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poult Sci 88(1): 49-56. https://doi.org/10.3382/ps.2008-00244
Aydogan I, Yildirim E, Kurum A, Bolat D, Cinar M, Basalan M, et al., 2020. The effect of dietary garlic (Allium sativum), black cumin (Nigella sativa) and their combination on performance, intestine morphometry, serum biochemistry and antioxidant status of broiler chickens. Braz J Poult Sci 22 (4): 1-10. https://doi.org/10.1590/1806-9061-2020-1317
Boka J, Mahdavi AH, Samie AH, Jahanian R, 2013. Effect of different levels of black cumin (Nigella sativa L.) on performance, intestinal Escherichia coli colonization and jejunal morphology in laying hens. J Anim Physiol Anim Nutr 98(2): 373-383. https://doi.org/10.1111/jpn.12109
COBB, 2012. Manual de manejo de frangos de corte Cobb. Cobb-Vantress Brasil Ltda: Guapiaçu. https://www.cobb-vantress.com/assets/Cobb-Files/df5655a7e9/Broiler-Guide-2019-POR-WEB.pdf
Corzo-Martínez M, Corzo N, Villamiel M, 2007. Biological properties of onions and garlic. Trends Food Sci 18(12): 609-625. https://doi.org/10.1016/j.tifs.2007.07.011
Dehghani-Tafti N, Jahanian R, 2016. Effect of supplemental organic acids on performance, carcass characteristics, and serum biochemical metabolites in broilers fed diets containing different crude protein levels. Anim Feed Sci Technol 211(1): 109-116. https://doi.org/10.1016/j.anifeedsci.2015.09.019
El-Katcha MI, Soltan MA, Sharaf MM, Hasen A, 2016. Growth performance, immune response, blood serum parameters, nutrient digestibility and carcass traits of broiler chicken as affected by dietary supplementation of garlic extract (Allicin). Alex J Vet Sci 49(2): 50-64. https://doi.org/10.5455/ajvs.219261
Elagib HAA, El-Amin WIA, Elamin KM, Malik HEE, 2013. Effect of dietary garlic (Allium sativum) supplementation as feed additive on broiler performance and blood profile. J Anim Sci Adv 3(2): 58-64. https://doi.org/10.5455/jasa.20130219104029
Elbaz AM, Ibrahim NS, Shehata AM, Mohamed NG, Abdel-Moneim AME, 2021. Impact of multi-strain probiotic, citric acid, garlic powder or their combinations on performance, ileal histomorphometry, microbial enumeration and humoral immunity of broiler chickens. Trop Anim Health Prod 53(1): 1-10. https://doi.org/10.1007/s11250-021-02554-0
Eltazi S, 2014. Response of broiler chicks to diets containing different mixture levels of garlic and ginger powder as natural feed additives. Int J Pharm Res Allied Sci 3 (4): 27-35. https://ijpras.com/storage/models/article/z06V5fYDMt29feBuayja09HwraAck3onyvAaSR0XSUHX7LLYqxfqfWdK4flo/response-of-broiler-chicks-to-diets-containing-different-mixture-levels-of-garlic-and-ginger-powde.pdf
Fadlalla IMT, Mohammed BH, Bakhiet AO, 2010. Effect of feeding garlic on the performance and immunity of broilers. Asian J Poult Sci 4(4): 182-189. https://doi.org/10.3923/ajpsaj.2010.182.189
Geravand M, Sharifi SD, Yaghobfar A, Mohammadi A, Hosseini SA, Ghazanfari S, 2021. Growth performance, ascites sensitivity, and ileal microbiota as affected by licorice essential oil in broiler chicken diets. Livest Sci 251(1): 1-6. https://doi.org/10.1016/j.livsci.2021.104670
Gotardo LR, Litz FH, Fernandes EDA, Souza MM, Saar AGL, Martins JMDS, 2016. Rendimento e composição bromatológica do peito de frangos de corte fêmeas de diferentes linhagens. Veterinária Notícias 22 (1): 32-37. https://doi.org/10.14393/VTv22n1a2016.28832
Haley PJ, 2017. The lymphoid system: a review of species differences. J Toxicol Pathol 30(2): 111-123. https://doi.org/10.1293/tox.2016-0075
Hayat SU, Ali R, Khan AH, Din IU, Khan S, Ullah F, 2022. Effect of garlic (Allium sativum) supplementation on growth performance and serum biochemistry of broiler chicks. Amer Acad Sci Res J Eng Technol Sci 85(1): 287-300. https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/7206
Herawati M, Marjuki A, 2011. The effect of feeding red ginger (Zingiber officinale rosc) as phytobiotic on broiler slaughter weight and meat quality. Int J Poul Sci 10(12): 983-985. https://doi.org/10.3923/ijps.2011.983.986
Herrera M, Gutiérrez O, Macías J, Savón L, Barrera A, Diaz A, 2017. Effects of the alimentary restriction and living space in the intake and digestive transit of naked neck chickens in grazing. Cuba J Agric Sci 51(3): 343-350. http://www.cjascience.com/index.php/CJAS/article/view/756/777
Ismail IE, Alagawany M, Taha AE, Puvača N, Laudadio V, Tufarelli V, 2021. Effect of dietary supplementation of garlic powder and phenyl acetic acid on productive performance, blood haematology, immunity and antioxidant status of broiler chickens. Anim Biosci 34(3): 363-370. https://doi.org/10.5713/ajas.20.0140
Jimoh, AA, Olorede BR, Abubakar A, Fabiyi JP, Ibitoye EB, Suleiman N, et al., 2012. Lipids profile and haematological indices of broiler chickens fed garlic (Allium sativum)-supplemented diets. J Vet Adv 2(10): 474-480. https://www.researchgate.net/profile/Emmanuel-bitoye/publication/303687003_Lipids_profile_and_Haematological_Indices_of_Broiler_Chickens_fed_Garlic_Allium_sativum_-_Supplemented_Diets/links/574e568908ae82d2c6be2cc6/Lipids-profile-and-Haematological-Indices-of-Broiler-Chickens-fed-Garlic-Allium-sativum-Supplemented-Diets.pdf
Khaligh F, Sadeghi G, Karimi A, Vaziry A, 2011. Evaluation of different medicinal plants blends in diets for broiler chickens. J Med Plant Res 5(10): 1971-1977.
Khan MSI, Prodhan MS, Islam MS, Hasan MN, Islam MS, 2017. Effect of garlic extract on growth performances and hematological parameters of broilers. Asian J Med Biol Res 3(3): 317-322. https://doi.org/10.3329/ajmbr.v3i3.34519
Kharde KR, Soujanya S. 2014. Effect of garlic and neem leaf powder supplementation on growth performance and carcass traits in broilers. Vet World 7(10): 799-802. https://doi.org/10.14202/vetworld.2014.799-802
Khoobani M, Hasheminezhad SH, Javandel F, Nosrati M, Seidavi A, Kadim IT, et al., 2020. Effects of dietary chicory (Chicorium intybus L.) and probiotic blend as natural feed additives on performance traits, blood biochemistry, and gut microbiota of broiler chickens. Antibiotics 9(1): 1-9. https://doi.org/10.3390/antibiotics9010005
Kumar A, Sharma NK, Kheravii SK, Keerqin C, Ionescu C, Blanchard A, et al., 2022. Potential of a mixture of eugenol and garlic tincture to improve performance and intestinal health in broilers under necrotic enteritis challenge. Anim Nutr 8(1): 26-37. https://doi.org/10.1016/j.aninu.2021.07.007
Lukanov H, Genchev A, Ribarski S, 2015. Effect of feed supplementation with garlic power on meat productivity and meat quality traits of classic Ross 308 male hybrid chickens. Trakia J Sci 13(1): 66-76. https://doi.org/10.15547/tjs.2015.01.009
Maynard CL, Elson CO, Hatton RD, Weaver CT, 2012. Reciprocal interactions of the intestinal microbiota and immune system. Nature 489(7415): 231-241. https://doi.org/10.1038/nature11551
Mickdam E, Alwaleed S, Madany M, Sayed A. 2022. The effect of moringa oleifera leaves on chicken meat composition and meat quality. Int J Vet Sci 11(2): 201-206. https://doi.org/10.47278/journal.ijvs/2021.094
Min YN, Yang HL, Xu YX, Gao YP, 2016. Effects of dietary supplementation of synbiotics on growth performance, intestinal morphology, sIgA content and antioxidant capacities of broilers. J Anim Physiol Anim Nutr 100(6): 1073-1080. https://doi.org/10.1111/jpn.12479
Mohamed SH, Attia AI, Reda FM, Abd El-Hack ME, Ismail IE, 2021. Impacts of dietary supplementation of Boswellia serrata on growth, nutrients digestibility, immunity, antioxidant status, carcase traits and caecum microbiota of broilers. Ital J Anim Sci 20(1): 205-214. https://doi.org/10.1080/1828051X.2021.1875336
Moreira J, Mendes AA, Roça RDO, Garcia EA, Naas IDA, Garcia RG, et al., 2004. Efeito da densidade populacional sobre desempenho, rendimento de carcaça e qualidade da carne em frangos de corte de diferentes linhagens comerciais. R Bras Zootec 33(6): 1506-1519. https://doi.org/10.1590/S1516-35982004000600018
Peinado MJ, Ruiz R, Echávarri A, Aranda-Olmedo I, Rubio LA, 2013. Garlic derivative PTS-O modulates intestinal microbiota composition and improves digestibility in growing broiler chickens. Anim Feed Sci Technol 181(1-4): 87-92. https://doi.org/10.1016/j.anifeedsci.2013.03.001
Perozo F, Nava J, Mavárez Y, Arenas E, Serje P, Briceño M, 2004. Morphometric characterization of Ross line broiler chickens lymphoid organs reared under field conditions in Zulia state. Rev Cient Vet 14(3): 217-225. https://www.researchgate.net/publication/286954909_Morphometric_characterization_of_ross_line_broiler_chickens_lymphoid_organs_reared_under_field_conditions_in_Zulia_State
R Core Team, 2019. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/.
Rahimi S, Teymori Zadeh Z, Torshizi K, Omidbaigi R, Rokni H, 2011. Effect of the three herbal extracts on growth performance, immune system, blood factors and intestinal selected bacterial population in broiler chickens. J Agr Sci Technol 13(4): 527-539. http://jast.modares.ac.ir/article-23-5732-en.pdf
Rehman Z, Munir MT, 2015. Effect of garlic on the health and performance of broilers. Veterinaria 3(1): 32-39. https://www.academia.edu/download/43720986/v3i1-7-2015260624.pdf
Rohollahzadeh H, Nili H, Asasi K, Mokhayeri S, Najjari AHA, 2018. Respiratory and GIT tract immune responses of broiler chickens following experimental infection with Newcastle disease's virus. Comp Clin Path 27(5): 1241-1255. https://doi.org/10.1007/s00580-018-2728-z
Rostagno HS, Albino LFT, Hannas MI, Donzele JL, Sakomura NK, Perazzo FG, et al., 2017. Tabelas brasileiras para aves e suínos: Composição de alimentos e exigências nutricionais, 4° Ed. Dept. de Zootecnia, Universidade Federal de Viçosa, Brasil.
Saleem MU, Javid MA, Kiani FA, Nazir MM, Bhatti AS, 2021. Carcass yield and histo-anatomical changes in broilers fed on diet supplemented with different levels of phytobiotics. Ind J Anim Res 55(10): 1184-1188. https://doi.org/10.18805/ijar.B-1118
Saleha AA, Myaing TT, Ganapathy KK, Zulkifli I, Raha R, Arifah K, 2009. Possible effect of antibiotic-supplemented feed and environment on the occurrence of multiple antibiotic resistant Escherichia coli in chickens. Int J Poult Sci 8(1): 28-31. https://doi.org/10.3923/ijps.2009.28.31
Samadi S, Wajizah S, Tarman A, 2020. Administration of Zingiber zerumbet extract on performances and haematological parameters of broiler chickens. IOP Conf Ser: Earth Environ Sci 497(1). https://doi.org/10.1088/1755-1315/497/1/012048
Samanthi KAM, Nayananjalie WAD, Adikari AMJB, Liyanage R, 2015. Dietary garlic (Allium sativum L.) supplementation on performance, meat quality and lipid profile in broilers. Raja Univ J 3(1): 17-24. http://repository.rjt.ac.lk/handle/123456789/34
Sangilimadan K, Churchil R, Premavalli K, Omprakash AV, 2019. Effect of garlic (Allium sativum) on production performances and carcass traits of Nandanam Broiler-2. Int J Curr Microbiol Appl Sci 8(4): 2531-2538. https://doi.org/10.20546/ijcmas.2019.804.295
Sheoran N, Kumar R, Kumar A, Batra K, Sihag S, Maan S, et al., 2017. Nutrigenomic evaluation of garlic (Allium sativum) and holy basil (Ocimum sanctum) leaf powder supplementation on growth performance and immune characteristics in broilers. Vet World 10(1): 121-129. https://doi.org/10.14202/vetworld.2017.121-129
Wijtten PJA, Langhout DJ, Verstegen MWA, 2012. Small intestine development in chicks after hatch and in pigs around the time of weaning and its relation with nutrition: A review. Acta Agric Scand A: Anim Sci 62(1): 1-12. https://doi.org/10.1080/09064702.2012.676061
Xie Z, Shen G, Wang Y, Wu C, 2019. Curcumin supplementation regulates lipid metabolism in broiler chickens. Poult Sci 98(1): 422-429. https://doi.org/10.3382/ps/pey315
Yaqoob MU, Abd El-Hack ME, Hassan F, El-Saadony MT, Khafaga AF, Batiha GE, et al., 2021. The potential mechanistic insights and future implications for the effect of prebiotics on poultry performance, gut microbiome, and intestinal morphology. Poult Sci 100(7): 101143. https://doi.org/10.1016/j.psj.2021.101143
Yegani M, Korver DR, 2008. Factors affecting intestinal health in poultry. Poult Sci 87(10): 2052-2063. https://doi.org/10.3382/ps.2008-00091
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