Chemical composition of a new Lupinus species found in Spain, Lupinus mariae-josephi H. Pascual (Fabaceae)
Abstract
A new lupin from Valencia (Spain) has been described recently. The new species Lupinus mariae-josephi H. Pascual (Fabaceae) differs from any other Mediterranean and North American taxon in banner position, flower colour and inflorescence structure. This species was found in a calcareous area, and when it was grown in experimental fields it has always performed better in alkaline soils. This work attempts to establish the content of protein, oil and nutritionally active factors (alkaloids, α-galactosides and inositol phosphates) in this new species of lupin, to investigate differences with other Lupinus ssp. The analysed raw seed samples of this new species showed a total protein content of 329.4 g kg–1 and oil content of 54.0 g kg–1. The total content of alkaloids, α-galactosides and phytate was similar to other bitter Lupinus species being lupanine, stachyose and myo-inositol hexaphosphate (IP6) the main bioactive compounds. However, the profile of alkaloids and proteins, which is an important chemotaxonomic criterion, was characteristic of this species. It is worth to notice that this Lupinus is able to grow in calcareous soils what makes this species very promising for soil recoveryDownloads
References
Álvarez-Álvarez J., Guillamón E., Crespo J.F., Cuadrado C., Burbano C., Rodríguez J., Fernández C., Muzquiz M., 2005. Effect of extrusion, boiling, autoclaving and microwave heating on lupine allergenicity. J Agr Food Chem 53, 1294-1298. http://dx.doi.org/10.1021/jf0490145
AOAC, 1990. Methods of analysis, 15th ed. Association of Official Analytical Chemists. Washington D.C.
Barrientos R.L., Vargas J.R., Huerta C.M., Navarro A.F., Garcia C.E.A., Huizar L.R., Segura N.M., 2006. Evaluation of seed protein content of a wild Mexican Lupin. Proc 11th Int Lupin Conf. Guadalajara, Jalisco, Mexico. pp. 176-177.
Blagrove R.J., Gillespie J.M., 1975. Isolation, purification and characterization of the seed globulins of Lupinus angustifolius. Aust J Plant Physiol 2, 13-27. http://dx.doi.org/10.1071/PP9750013
Bradford M.M., 1976. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72, 248-254. http://dx.doi.org/10.1016/0003-2697(76)90527-3
Burbano C., Muzquiz M., Osagie A., Ayet G., Cuadrado C., 1995. Determination of phytate and lower inositol phosphates in Spanish legumes by HPLC methodology. Food Chem 52, 321-325. http://dx.doi.org/10.1016/0308-8146(95)92831-4
CASTROVIEJO S., PASCUAL H., 1999. Lupinus L. In: Flora Ibérica vol. VII (1) (Talavera S., ed.). Real Jardín Botánico, CSIC, Madrid, Spain. pp. 251-260.
Cosgrove D.J., 1980. Nutritional aspects of inositol phosphates. In: Inositol phosphates. Their chemistry, biochemistry and physiology (Cosgrove D.J., ed.). Elsevier Sci Publ, Amsterdam, The Netherlands. pp. 139-156.
Crittenden R.G., Playne M.J., 1996. Production, properties and applications of food-grade oligosaccharides. Trends Food Sci Tech 7, 353-361. http://dx.doi.org/10.1016/S0924-2244(96)10038-8
De La Cuadra C., Muzquiz M., Burbano C., Ayet G., Calvo R., Osagie A., Cuadrado C., 1994. Alkaloid, α-galactoside and phytic acid changes in germinating lupin seeds. J. Sci Food Agric 66, 357-364. http://dx.doi.org/10.1002/jsfa.2740660313
Emre I., Turgut-Balik D., Genc H., Sahin A., 2006. The use of seed proteins revealed by SDS-PAGE in taxonomy of some Lathyrus L. Species grown in Turkey. Pak J Biol Sci 9, 2358-2361. http://dx.doi.org/10.3923/pjbs.2006.2358.2361
Esnault M.A., Merceur A., Cithard J., 1991. Characterization of globulins of yellow lupin seeds. Plant Physiol Biochem 29, 573-583.
Frias J., Vidal-Valverde C., Kozlowska H., Gorezki R., Honke J., Hedley C.L., 1996. Changes in monosaccharides, disaccharides and α-galactosides during development of legume seeds. In: Effects of antinutrients on the nutritional value of legume diets (Bardocz S., Gelencser E. and Pusztai A., eds.). European Commission Publication, Luxembourg. pp. 118-123.
Fuentes E., Planchuelo A.M., 2006. Phytochemical characterization of wild and cultivated species of Lupinus in relation to species of other genera of Genisteae (Fabaceae). Proc. 11th Int Lupin Conf. Guadalajara, Jalisco, Mexico. pp. 157-168.
Garcia-Lopez P.M., Muzquiz M., Ruiz-Lopez M.A., Zamora-Natera J.F., Burbano C., Pedrosa M.M., Cuadrado C., Garzon-De La Mora P., 2001. Chemical composition and fatty acid profile of several Mexican wild lupins. J Food Compos Anal 14, 1-7.
Greiner R., Larsson-Alminger M., Carlsson N.G., Muzquiz M., Burbano C., Cuadrado C., Pedrosa M.M., Goyoaga, C., 2002. Pathway of dephosphorylation of myoinositol hexakisphosphate by phytases of legume seeds. J Agric Food Chem 50, 6865-6870. http://dx.doi.org/10.1021/jf025620t
Grizard D., Barthomeu F.C., 1999. Non-digestible oligosaccharides used as prebiotic agents: mode of production and beneficial effects on animal and human health. Reprod Nutr Dev 39, 563-588. http://dx.doi.org/10.1051/rnd:19990505
Horbowicz M., Obendorf R.L., 1994. Seed desiccation tolerance and storability: dependence on flatulence-producing oligosaccharides and cyclitols- review and survey. Seed Sci Res 4, 385-405. http://dx.doi.org/10.1017/S0960258500002440
Jha S.S., Ohri D., 2002. Comparative study of seed protein profiles in the genus Pisum. Biol Plant 45, 529-532. http://dx.doi.org/10.1023/A:1022316907401
JIMENEZ-MARTINEZ C., PEDROSA M.M., MUZQUIZ M., DAVILA-ORTIZ G., 2004. Elimination of quinolizidine alkaloids, α-galactosides and phenolic compounds from Lupinus campestris seed by aqueous, acid and alkaline thermal treatment. In: Recent advances of research in antinutritional factors in legume seeds and oilseeds (Muzquiz M. et al., eds.). EAAP publication, Toledo, Spain. pp. 343-346.
Kadlec P., 2000. Carbohydrate chemistry. In: Carbohydrates in grain legumes seeds, improving nutritional quality and agronomic characteristics (Hedley C.L., ed.). CABI Publ, Norwich, UK. pp. 15-59. http://dx.doi.org/10.1079/9780851994673.0015
Kvasnicka F., Ahmadova-Vavrousova R., Mrskos M., Velisek J., Kadlec P., 1994. Characteristics of pea varieties according to galactooligosaccharides content. In: Agricultural food quality. An interdisciplinary approach (Fenwick G.R. et al, eds.). The Royal Society of Chemistry, Cambridge, UK. pp.153-156.
Ladizinsky G., Hymowitz T., 1979. Seed protein electrophoresis in taxonomic and evolutionary studies. Theor Applied Genet 54, 145-151. http://dx.doi.org/10.1007/BF00263044
Laemmli U K., 1970. Clevage of structural proteins during the assembly of the head of bacteriophage T4. Nature 15, 227-680.
Lahuta L.B., Jagielska T., Gorecki R.J., Jones A., Hedley C., 1998. Soluble carbohydrates in desiccation tolerance of germinating pea seeds of different isolines. Proc 3rd Europ Conf on Grain Legumes, Valladolid, Spain. Pp. 40-41.
Lehrfeld J., 1994. HPLC separation and quantification of phytic acid and some inositol phosphates in foods: problems and solutions. J Agr Food Chem 42, 2726-2731. http://dx.doi.org/10.1021/jf00048a015
Leterme P., 2002. Recommendations by health organizations for pulse consumption. Br J Nutr 88, 239-242. http://dx.doi.org/10.1079/BJN2002712
Lowell C.A., Kuo T.M., 1989. Oligosaccharide metabolism and accumulation in developing soybean seeds. Crop Sci 29, 459-465. http://dx.doi.org/10.2135/cropsci1989.0011183X002900020044x
Mahé F., Pascual H., Coriton O., Huteau V., Navarro-Perris A., MISSET M T., AINOUCHE A., 2011. New data and phylogenetic placement of the enigmatic Old World lupin: Lupinus mariae-josephi H. Pascual. Genet Resour Crop Evol 58(1), 101-114. http://dx.doi.org/10.1007/s10722-010-9580-6
Martinez-Dominguez B., Ibañez-Gomez M.V., Rincón-León F., 2002. Acido fítico: aspectos nutricionales e implicaciones analíticas. Arch Latin Nutr 52, 1-22. [In Spanish]. MEIßNER C., WINK, M., 1992. GM/MS Analyse von Alkaloiden Nordamerikanischer Lupinen. In: Lupinen 1991-Forschung, Anbau und Verwertung (Wink M., ed). Universitat Heidelberg, Heidelberg, Germany. pp. 91-129. [In German].
Melo S.T., Ferreira R.B., Teixeira A.N., 1994. The seed storage proteins from Lupinus albus. Phytochem 3, 641-648. http://dx.doi.org/10.1016/S0031-9422(00)90331-5
MORRISON WR., SMITH LM., 1964. Preparation of fatty acid methyl esters and dimethyl acetals from lipids with boron fluoride-methanol. J Lipid Res 5, 600-608.
Mossé J., Pernollet J.C., 1983 Storage protein of legume seeds. In: Chemistry and Biochemistry of Legumes (Arora S.K., ed.). Arnold E., London, UK. pp. 111-193
Mühlbauer P., Witte L., Wink M., 1987. New ester alkaloids from lupins. Planta Med 88, 237-239.
Muzquiz M., Burbano C., Gorospe M.J., Ródenas I., 1989. A chemical study of Lupinus hispanicus seed. Toxic and antinutritional components. J Sci Food Agr 47, 205-214. http://dx.doi.org/10.1002/jsfa.2740470208
Muzquiz M., Rey C., Cuadrado C., Fenwick R., 1992. Effect of germination on oligosaccharide content of lupin species. J Chromatogr 607, 349-352. http://dx.doi.org/10.1016/0021-9673(92)87094-O
Muzquiz M., Burbano C., Cuadrado C., De La Cuadra C., 1993. Determinación de factores antinutritivos termorresistentes en leguminosas. I. Alcaloides. Invest Agrar: Prod Prot Veg 8(3), 351-361. [In Spanish].
Muzquiz M., Cuadrado C., Ayet G., De La Cuadra C., Burbano C., Osagie A., 1994. Variation of alkaloid components of lupin seeds in 49 genotypes of Lupinus albus L. from different countries and locations. J Agr Food Chem 42(7), 1447-1450. http://dx.doi.org/10.1021/jf00043a011
Muzquiz M., Burbano C., Pedrosa M.M., Folkman W., Gulewicz K., 1999a. Lupins as a potential source of raffinose family oligosaccharides. Preparative method of their isolation and purification. Ind Crop Prod 19, 183-188. http://dx.doi.org/10.1016/S0926-6690(98)00030-2
Muzquiz M., Burbano C., Ayet G., Pedrosa M.M., Cuadrado C., 1999b. The investigation of antinutritional factors in Phaseolus vulgaris. Environmental and varietal differences. Biotech Agro Society Environ 3, 210-216.
Muzquiz M., Burbano C., 2005. Bioactive compounds in Lupinus spp. Implications for nutrition and health. In: Mexico, where old and new world lupins meet (van Santen E. and Hill G.D., eds.). Proc 11th Int Lupin Conf, Guadalajara, Jalisco, Mexico. pp 327-340.
Naganowska B., Wolko B., Sliwinska E., Kaczmarek Z., 2003. Nuclear DNA content variation and species relationships in the genus Lupinus (Fabaceae). Ann Bot 92, 349-355. http://dx.doi.org/10.1093/aob/mcg145
Obendorf R.L., 1997. Oligosaccharides and galactosyl cyclitols in seed desiccation tolerance. Seed Sci Res 7, 63-74 http://dx.doi.org/10.1017/S096025850000341X
Pascual H., 2004. Lupinus mariae-josephi (Fabaceae), nueva y sorprendente especie descubierta en Espa-a. Anales del Jardín Botánico de Madrid 61 (1), 61-92. [In Spanish]. http://dx.doi.org/10.3989/ajbm.2004.v61.i1.69
Piotrowicz-Cieslak A.I., 2004. Flatulence-producing galactosyl cyclitols D-chiro-inositol fraction in maturing yellow lupin seed. In: Recent advances of research in antinutritional factors in legume seeds and oilseeds (Muzquiz M. et al., eds.). EAAP publication, Toledo, Spain. pp. 69-72.
Piotrowicz-Cieslak A.I., Gorecki R.J., Adomas B., 1999. The content and composition of soluble carbohydrates in lupin seeds of different species and cultivars. Plant Breed Seed Sci 43, 25-34.
Piotrowicz-Cieslak A.J., Garcia-Lopez P.M., Gulewicz K., 2003. Cyclitols, galactosyl cyclitols and raffinose family oligosaccharides in mexican wild lupin seeds. Acta Societatis Botanicorum Poloniae 72, 109-114. http://dx.doi.org/10.5586/asbp.2003.014
Przybylak J.K., Ciesiolka D., Wysocka W., Garcia-Lopez P.M., Ruiz-Lopez M.A., Wysocki W., Gulewicz K., 2005. Alkaloid profiles of Mexican wild lupin and a effect of alkaloid preparation from Lupinus exaltatus seeds on growth and yield of paprika (Capsicum annuum L.). Ind Crop Prod 21, 1-7. http://dx.doi.org/10.1016/j.indcrop.2003.12.001
Pusztai A., Bardoc S., Martin-Cabrejas M.A., 2004. The mode of action of ANFs on the gastrointestinal tract and its microflora. In: Recent advances of research in antinutritional factors in legume seeds and oilseeds (Muzquiz M. et al., eds). EAAP publication, Toledo, Spain. pp. 87-100.
Quemener B., 1988. Improvements in the high-pressure liquid chromatography determination of the amino sugars and α-galactosides in faba bean, lupine and pea. J Agric Food Chem 36, 754-759. http://dx.doi.org/10.1021/jf00082a020
Ruiz-Lopez M.A., GARCIA-LOPEZ PM., CASTAÑEDA-VAZQUEZ H., ZAMORA N.J.F., GARZÓN P., BAÑUELOS PINEDA J., BURBANO C., PEDROSA MM., CUADRADO C., MUZQUIZ M., 2000. Chemical composition and antinutrient content of three Lupinus species from Jalisco, Mexico. J Food Compos Anal 13, 193-199. http://dx.doi.org/10.1006/jfca.1999.0887
Scarafoni A., Dicataldo C., Magm C., Duranti M., 2004. Lupin seeds as a source of nutraceuticals. In: Wild and cultivated lupins from tropics to the poles (Van Santen E. and Hill G.D., eds). Proc. 10th Int Lupin Conf, Laugarvatn, Iceland. pp. 352-355.
Simopoulosa P., 2006. Evolutionary aspects of diet, the omega-6/omega-3 ratio and genetic variation: nutritional implications for chronic diseases. Biomed Pharmacother 60, 502-507. http://dx.doi.org/10.1016/j.biopha.2006.07.080
Tomomatsu H., 1994. Health effects of oligosaccharides. Food Tech 48, 61-65.
Trugo L.C., Almeida D.C.F., Gross R., 1988. Oligosaccharide contents in the seeds of cultivated lupins. J Sci Food Agric 45, 21-24. http://dx.doi.org/10.1002/jsfa.2740450104
Urbano G., López-Jurado M., Aranda P., Vidal-Valverde C., Tenorio E. J., Porres E., 2000. The role of phytic acid in legumes: antinutrient or beneficial function? J Physiol Biochem 56, 283-294. http://dx.doi.org/10.1007/BF03179796
Vaz A.C., Pinheiro C., Martins J.M.N., Ricardo C.P.P., 2004. Cultivar discrimination of Portuguese Lupinus albus by seed protein electrophoresis: The importance of considering glutelins and glycoproteins. Field Crops Res 87, 23-34. http://dx.doi.org/10.1016/j.fcr.2003.09.006
Wink M., MEIßNER C., WITTE L., 1995. Patterns of quinolizidine alkaloids in 56 species of the genus Lupinus. Phytochem 18, 139-153. http://dx.doi.org/10.1016/0031-9422(95)91890-D
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