Effect of grinding size and sunflower oil addition on intake, digestibility, rumen function and microbial protein synthesis in sheep fed a dry total mixed ration
Abstract
An experiment was carried out in which the effect of grinding a dry total mixed ration, based on barley straw, through 6 or 10 mm sieves on intake, digestibility, rumen function and microbial protein synthesis in sheep was assessed. The effect of including 1% sunflower oil as binder was also explored. None of the variables studied was affected by grinding size, hence the 10 mm sieve was recommended due to its lower cost in terms of energy consumed. On the contrary, addition of 1% sunflower oil significantly increased ammonia concentration in the rumen (83 vs. 161 mg L–1; p = 0.002), potential degradabilities (as proportion) of dry matter (0.65 vs. 0.75; p = 0.0037), organic matter (0.68 vs. 0.75; p = 0.0005) and crude protein (0.78 vs. 0.90; p = 0.0004), and fractional rate of degradation (0.066 vs. 0.080 h–1; p = 0.046) and effective degradability, as proportion (0.63 vs. 0.73; p = 0.010), of this latter. Oil addition (1%) is then recommended as binder, although a more economical option than sunflower oil should be explored due to its high cost.
Downloads
References
Aitchison E.M., Gill M., Dhanoa M.S., Osbourn D.F., 1986. The effect of the digestibility of forage species on the removal of digesta from the rumen and the voluntary intake of hay by sheep. Br J Nutr 56, 463-476. http://dx.doi.org/10.1079/BJN19860126 PMid:3676225
Allen M.S., 1996. Physical constraints on voluntary intake of forages by ruminants. J Anim Sci 74, 3063-3075. PMid:8994921
Allen M.S., 1997. Relationships between fermentation acid production in the rumen and the requirement for physically effective fiber. J Dairy Sci 80, 1447-1462. http://dx.doi.org/10.3168/jds.S0022-0302(97)76074-0
AOAC, 2005. Official Methods of Analysis of AOAC International, 18th ed. Association of Official Analytical Chemists, Gaithersburg, MD, USA.
Balcells J., Guada J.A., Castrillo C., Peiró J.M., 1992. Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography. J Chromatogr 575, 153-157.
Balch C.C., Campling R.C., 1962. Regulation of voluntary food intake in ruminants. Nutr Abstr Rev B 32, 669-686.
Busquet M., Calsamiglia S., Ferret A., Cardozo P.W., Kamel C., 2005. Effects of cinnamaldehyde and garlic oil on rumen microbial fermentation in a dual flow continuous culture. J Dairy Sci 88, 2508-2516. http://dx.doi.org/10.3168/jds.S0022-0302(05)72928-3
Cassida K.A., Stokes M.R., 1986. Eating and resting salivation in early lactation dairy cows. J Dairy Sci 69, 1282-1291. http://dx.doi.org/10.3168/jds.S0022-0302(86)80534-3
Chaney A.L., Marbach E.P., 1962. Modified reagents for determination of urea and ammonia. Clin Chem 8,130. PMid:13878063
Conrad H.R., Pratt A.D., Hibbs J.W., 1964. Regulation of feed intake in dairy cows. 1- Change in importance of physical and physiological factors with increasing digestibility. J Dairy Sci 47, 54-62. http://dx.doi.org/10.3168/jds.S0022-0302(64)88581-7
Cruickshank G.J., Poppi D.P., Sykes A.R., Familton A.S., 1990. Effect of age, abomasal cannulation and rumen catheterization on intake and site of digestion by early-weaned lambs. J Agric Sci 114, 49-54. http://dx.doi.org/10.1017/S0021859600070982
De Vega A., Poppi D.P., 1997. Extent of digestion and rumen condition as factors affecting passage of liquid and digesta particles in sheep. J Agric Sci 128, 207-215. http://dx.doi.org/10.1017/S0021859696004078
Downes A.M., Mcdonald I.W., 1964. The chromium-51 complex of ethylenediamino tetraacetic acid as a soluble rumen marker. Br J Nutr 18, 153-162. http://dx.doi.org/10.1079/BJN19640015 PMid:14112964
Emanuele S.M., Staples C.R., 1988. Effect of forage particle size on in situ digestion kinetics. J Dairy Sci 71, 1947-1954. http://dx.doi.org/10.3168/jds.S0022-0302(88)79765-9
Faichney G.J., 1983. The effect of physical form of lucerne hay on the passage of markers through the rumen of sheep. Proc Nutr Soc Aust 8, 186.
Faichney G.J., 1993. Digesta flow. In: Quantitative aspects of ruminant digestion and metabolism (Forbes J.M., France J., eds.). CAB Int, Wallingford, UK. pp. 53-85.
Fondevila M., Castrillo C., Gasa J., 1993. Effect of ammonia treatment of barley straw on the dynamics of its degradation in the rumen. Anim Prod 57, 407-413.
Fondevila M., Castrillo C., Guada J.A., Balcells J., 1994. Effect of ammonia treatment and carbohydrate supplementation of barley straw on rumen liquid characteristics and substrate degradation by sheep. Anim Feed Sci Technol 50, 137-155. http://dx.doi.org/10.1016/0377-8401(94)90015-9
Fraser G.R., Chaves A.V., Wang Y., Mcallister T.A., Beauchemin K.A., Benchaar C., 2007. Assessment of the effects of cinnamon leaf oil on rumen microbial fermentation using two continuous culture systems. J Dairy Sci 90, 2315-2328. http://dx.doi.org/10.3168/jds.2006-688 PMid:17430934
Gómez-Cortés P., Frutos P., Mantecón A.R., Juárez M., Fuente M.A. De La, Hervás G., 2009. Effect of supplementation of grazing dairy ewes with a cereal concentrate on animal performance and milk fatty acid profile. J Dairy Sci 92, 3964-3972. http://dx.doi.org/10.3168/jds.2009-2044 PMid:19620680
Grovum W.L., Williams V.J., 1973. Rate of passage of digesta in sheep. 4-Passage of marker through the alimentary tract and the biological relevance of rate constants derived from the changes in concentration of marker in faeces. Br J Nutr 30, 313-329. http://dx.doi.org/10.1079/BJN19730036 PMid:4201027
Haghdoost A., Shadparvar A.A., Nasiri M.T.B., Fayazi J., 2008. Estimates of economic values for traits of Arabic sheep in village system. Small Rum Res 80, 91-94. http://dx.doi.org/10.1016/j.smallrumres.2008.08.001
Jouany J.P., 1982. Volatile fatty acid and alcohol determination in digestive contents, silage juices, bacterial cultures and anaerobic fermentor contents. Sci Aliments 2, 131-144.
Keli A., Andueza D., De Vega A., Guada J.A., 2008. Validation of the n-alkane and NIRS techniques to estimate intake, digestibility and diet composition in sheep fed mixed lucerne:ryegrass diets. Livestock Sci 119, 42-54. http://dx.doi.org/10.1016/j.livsci.2008.02.011
Lammers B.P., Buckmaster D.R., Heinrichs A.J., 1996. A simplified method for the analysis of particle sizes of forage and total mixed rations. J Dairy Sci 79, 922-928. http://dx.doi.org/10.3168/jds.S0022-0302(96)76442-1
Lock A.L., Rovai M., Gipson T.A., Veth M.J. De, Bauman D.E., 2008. A conjugated linoleic acid supplement containing trans-10, cis-12 conjugated linoleic acid reduces milk fat synthesis in lactating goats. J Dairy Sci 91, 3291-3299. http://dx.doi.org/10.3168/jds.2008-1071 PMid:18765588
Mayes R.W., Lamb C.S., Colgrove P.M., 1986. The use of dosed and herbage n-alkanes as markers for the determination of herbage intake. J Agric Sci 107, 161-170. http://dx.doi.org/10.1017/S0021859600066910
Mertens D.R., 2000. Physically effective NDF and its use in dairy rations explored. Feedstuffs, April 10, 11-14.
Mertens D.R., 2002. Gravimetric determination of amylase-treated neutral detergent fibre in feeds with refluxing beakers or crucibles: collaborative study. J Off Assoc Chem Int 85, 1217-1240.
National Research Council, 2001. Nutrient requirements of dairy cattle, 7th revised ed. National Academic Science, Washington, DC, USA.
Ørskov E.R., Mcdonald I., 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J Agric Sci 92, 499-503. http://dx.doi.org/10.1017/S0021859600063048
Patterson H.B.W., 1989. Handling and storage of oilseeds, oils, fats and meal. Elsevier Applied Science, Barking, UK. 394 pp.
Pérez J.F., Rodriguez C., Gonzalez J., Balcells J., Guada J.A., 1996. Contribution of dietary purine bases to duodenal digesta in sheep. In situ studies of purine degradability corrected for microbial contamination. Anim Feed Sci Technol 62, 251-262. http://dx.doi.org/10.1016/S0377-8401(96)00961-3
Pinos-Rodríguez J.M., Moreno R., González S.S., Robinson P.H., Mendoza G., Álvarez G., 2008. Effects of exogenous fibrolytic anzymes on ruminal fermentation and digestibility of total mixed rations fed to lambs. Anim Feed Sci Technol 142, 210-219. http://dx.doi.org/10.1016/j.anifeedsci.2007.08.005
Pond K.R., Tolley E.A., Ellis W.C., Mathis J.H., 1984. A method for describing the weight distribution of particles from sieved forage. In: Techniques in particles size analysis of feed and digesta in ruminants (Kennedy P.M., ed.). Canadian Society of Animal Science, occasional publication 1, Edmonton, Alberta, Canada. pp. 123-133.
Poppi D.P., Norton B.W., Minson D.J., Hendricksen R.E., 1980. The validity of critical size theory for particles leaving the rumen. J Agric Sci 94, 275-280. http://dx.doi.org/10.1017/S0021859600028859
Robertson J.B., Van Soest P.J., 1981. The detergent system of analysis. In: The analysis of dietary fibre in food (James W.P.T., Theander O., eds.). Marcel Dekker, NY, USA. pp. 123-158.
Satter L.D., Slyter L.L., 1974. Effect of ammonia concentration on rumen microbial protein production in vitro. Br J Nutr 32, 199-208. http://dx.doi.org/10.1079/BJN19740073 PMid:4472574
Tufarelli V., Dario M., Laudadio V., 2009. Milk yield and composition of lactating Comisana ewes fed total mixed rations containing nitrogen sources with different ruminal degradability. Livestock Sci 122, 349-353. http://dx.doi.org/10.1016/j.livsci.2008.08.012
Ulyatt M.J., Dellow A.J., John A., Reid C.S.W., Waghorn G.C., 1986. Contribution of chewing eating and rumination to the clearance of digesta from the ruminoreticulum In: Control of digestion and metabolism in ruminants (Milligan L.P., Grovum W.L., Dobson A., eds.). Reston Publishing Co., Reston, Va, USA. pp. 498-515.
Vicente F., Guada J.A., Balcells J., Castrillo C., 2004. Microbial contribution to duodenal purine flow in fattening cattle fed concentrate diets estimated by purine N labelling (15N) of different microbial fractions. Anim Sci 78, 159–167.
Wiseman J., 1984. Fats in animal nutrition. Butterworths, London, UK. 521 pp.
Wolfová M., Wolf J., Krupová Z., Kica J., 2009. Estimation of economic values for traits of dairy sheep. J Dairy Sci 92, 2183-2194. http://dx.doi.org/10.3168/jds.2008-1411 PMid:19389977
© CSIC. Manuscripts published in both the printed and online versions of this Journal are the property of Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.
All contents of this electronic edition, except where otherwise noted, are distributed under a “Creative Commons Attribution 4.0 International” (CC BY 4.0) License. You may read here the basic information and the legal text of the license. The indication of the CC BY 4.0 License must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the published by the Editor, is not allowed.