Short communication. Isolation of Aeromonas hydrophila in piglets
Abstract
The production of Alentejano breed pig started a recovery two decades ago due to increasing demand for gourmet products. These pigs are raised in rotational semi-extensive or extensive outdoor production systems in the “Montado” (green and cork oak forest), grazing and feeding acorns and other associated food resources. Bacteria of the genus Aeromonas are considered as emerging pathogens of importance for man and animals, but its involvement in swine is not well documented. In the context of a study made at the University of Évora to assess the specific diseases of Alentejano swine, diseased piglets from two farms were submitted for pathological and bacteriological examinations. Pathological examinations revealed changes characteristic of septicemia, and Aeromonas hydrophila was isolated in pure culture from multiple organs of piglets from both farms. Antibiotic sensitivity tests showed that the isolates from one of the farms were susceptible to gentamicin, oxitetracycline, neomycin, enrofloxacin, colistin sulfate, trimethoprim, ceftiofur, and amoxicillin plus clavulanic acid. In contrast, the A. hydrophila isolated in the other farm was resistant to all drugs tested but enrofloxacin. This is the first report in the world showing the relationship between septicemia and A. hydrophila infection in piglets. The importance of this finding is further reinforced by the fact that these bacteria can be highly resistant to antimicrobial agents.Downloads
References
Abdel-Gwad AM, Abdel-Rahman AA, 2004. Isolation and significance of Aeromonas hydrophila group in farmed rabbits at Assiut Governorate. Assiut University B Environ Res 7(1): 85-93.
Akkoç A, Kocabiyik AL, Özyigit MO, Cangül IT, Yilmaz R, Özakin C, 2008. Burkholderia cepacia and Aeromonas hydrophila septicemia in an African grey parrot (Psittacus erithacus erithacus). Turk J Vet Anim Sci 32(3): 233-236.
Brandi G, Sisti M, Schiavano GF, Salvaggio L, Albano A, 1996. Survival of Aeromonas hydrophila, Aeromonas caviae, and Aeromonas sobria in soil. J Appl Bacteriol 81(4): 439-444.
Charneca R, Nunes JLT, Le Dividich J, 2010. Body composition and blood parameters of newborn piglets from Alentejano and conventional (Large-White × Landrace) genotype. Span J Agric Res 8(2): 317-325.
CLSI, 2006. Performance standards for antimicrobial disc and dilution susceptibility tests for bacteria isolated from animals, approved standards, 3rd ed. Document M31-A3. Clinical and Laboratory Standards Institute, Wayne, PA, USA.
Costa FN, Rossi OD Jr, 2002. Bactérias do género Aeromonas em abatedouro de frangos. Arq Bras Med Vet Zoo 54(5), 534-535. [In Portuguese]. http://dx.doi.org/10.1590/S0102-09352002000500013
Daily O, Joseph S, Coolbaugh J, Walker R, Merrell B, Rollins D, Seidler R, Colwell R, Lissner C, 1981. Association of Aeromonas sobria with human infection. J Clin Microbiol 13(4): 769-777. PMid:6112237 PMCid:273875
Fontes MC, 2009. Diversidade filogenética e resistência a antibióticos em Aeromonas spp. isoladas de suínos abatidos para consumo de alheiras. Doctoral theses. Univ. Trás-os-Montes e Alto Douro, Vila Real, Portugal. [In Portuguese].
Freitas A, Neves J, Nunes JT, Martins J, 2006. O sistema agro-silvo-pastoril da raça suína Alentejana. IV Congreso Latinoamericano de Agroforesteria para la producción Pecuaria Sostenible. 24–27 Octubre. Cuba. [In Portuguese].
Fujii Y, Tsurumi K, Sato M, Takahashi E, Okamoto K, 2008. Fluid secretion caused by aerolysin-like hemolysin of Aeromonas sobria in the intestines is due to stimulation of production of prostaglandin E2 via cyclooxygenase 2 by intestinal cells. Infect Immun 76(3): 076-1082.
Ghenghesh KS, Abeid SS, Jaber MM, Bem-Taher SA, 1999. Isolation and haemolitic activity of Aeromonas species from domestic dogs and cats. Comp Immunol Microb 22: 175-179. http://dx.doi.org/10.1016/S0147-9571(98)00134-9
Gill CO, Jones T, 1995. The presence of Aeromonas, Listeria and Yersinia in carcass processing equipment at two pig slaughtering plants. Food Microbiol 12: 1-7. http://dx.doi.org/10.1016/S0740-0020(95)80089-1
Glünder G, Siegmann O, 1989. Ocurrence of Aeromonas hydrophila in wild birds. Avian Pathol 18: 685-695. http://dx.doi.org/10.1080/03079458908418642 PMid:18679900
Hinton A Jr, Cason JA, Ingram KD, 2004. Tracking spoilage bacteria in commercial poultry processing and refrigerated storage of poultry carcasses. Int J Food Microbiol 91: 155-165. http://dx.doi.org/10.1016/S0168-1605(03)00377-5
Ílhan Z, Gülhan T, Aksakal A, 2006. Aeromonas hydrophila associated with ovine abortion. Small Ruminant Res 61: 73-78. http://dx.doi.org/10.1016/j.smallrumres.2005.03.008
Janda JM, Abbott SL, 2010. The genus Aeromonas: taxonomy, pathogenicity, and infection. Clin Microbiol Rev 23(1): 35-73. http://dx.doi.org/10.1128/CMR.00039-09 PMid:20065325 PMCid:2806660
Krovacek K, Huang K, Sternberg S, Svenson SB, 1998. Aeromonas hydrophila septicaemia in a grey seal (Halichoerus grypus) from the Baltic Sea: a case study. Comp Immunol Microb 21: 43-49. http://dx.doi.org/10.1016/S0147-9571(97)00015-5
Mano SB, Ordoñez JA, Garcia de Fernando GD, 2000. Growth/survival of natural flora and Aeromonas hydrophila on refrigerated uncooked pork and turkey packaged in modified atmospheres. Food Microbiol 17: 657-669. http://dx.doi.org/10.1006/fmic.2000.0358
Martin-Carnahan AM, Joseph SW, 2005. Genus I. Aeromonas Stanier 1943, 213AL. In: Bergey`s manual of systematic bacteriology, 2nd ed, vol. 2. (Brenner DJ, Krieg NR, Staley JT & Garrity GM, eds). Springer, NY, pp: 557-578.
Moro EMP, Weiss RWN, Friedrich RSC, Vargas AC, Weiss LHN, Nunes MP, 1999. Aeromonas hydrophila isolated from cases of bovine seminal vesiculitis in South Brazil. J Vet Diagn Invest 11: 189-191. http://dx.doi.org/10.1177/104063879901100217 PMid:10098696
Nunes JT, 2007. Produção pecuária no montado – suínos. Rev Ciências Agrárias 30(1): 251 -259. [In Portuguese].
Pereira CS, Amorim SD, Santos AFM, Siciliano S, Moreno IB, Ott PH, Rodrigues DP, 2008. Plesiomonas shigelloides and Aeromonadaceae family pathogens isolated from marine mammals of Southern and Southeastern Brazilian Coast. Braz J Microbiol 39: 749-755. http://dx.doi.org/10.1590/S1517-83822008000400029
Queiroga MC, Branco S, Sepulveda F, Cortes H, Padre L, Morais Pinto A, Potes ME, 2009. Plano de estudo epidemiológico do porco alentejano e cruzado. Suinicultura 81: 34-41. [In Portuguese].
Roberts MTM, Enoch DA, Harris KA, Karas JA, 2006. Aeromonas veronii biovar sobria bacteraemia with septic arthritis confirmed by 16S rDNA PCR in an immunocompetente adult. J Med Microbiol 55: 241-243. http://dx.doi.org/10.1099/jmm.0.46295-0 PMid:16434719
Schneider RK, 1998. Common bacteria encountered in septic arthritis. P Annu Conv Am Equin 44: 152-158.
Staples P, 2000. Aeromonas, Plesiomonas and Vibrio bacteria isolated from animals in New Zealand. Surveillance 27(1): 3-4.
Talon D, Mulin B, Thouverez M, 1998. Clonal identification of Aeromonas hydrophila strains using randomly amplified polymorphic DNA analysis. Eur J Epidemiol 14: 305-310. http://dx.doi.org/10.1023/A:1007441019821 PMid:9663524
Tollefson L, Karp B, 2004. Human health impact from antimicrobial use in food animals. Med Maladies Infect 34(11): 514–521. PMid:15620055
Yáñez MA, Catalán V, Apráiz D, Figueras MJ, Martínez-Murcia AJ, 2003. Phylogenetic analysis of the members of the genus Aeromonas based on gyrB gene sequences. Int J Syst Evol Micr 53: 875-883. http://dx.doi.org/10.1099/ijs.0.02443-0
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