Inhibitory action of Lippia gracilis Schauer essential oil on pathogenic bacteria and its effects as a growth promoter on quail
Abstract
Aim of the study: To examine the in vitro sensitivity of Salmonella sp. and Escherichia coli strains to the microbial activity of Lippia gracilis Schauer essential oil (LGSEO) and to determine the optimal level of LGSEO as a growth promoter in diets for Japanese quail up to 35 days of age.
Area of study: São Cristovão, Sergipe, Brazil.
Material and methods: A total of 504 female Japanese quails (Coturnix coturnix japonica) at an initial average body weights of 6.80±0.10 g was allotted to one of six treatments (0, 100, 200, 300, 400 mg/kg of LGSEO and a diet containing 500 mg/kg of bacitracin methylene disalicylate) in 7 replicates, using 12 birds per experimental unit.
Main results: In the age period of 21 to 35 days, feed intake declined linearly (p=0.04) and feed efficiency improved (p<0.01), whereas no changes were observed in production performance (p>0.05). The estimated (p=0.01) maximum relative weights of proventriculus and pancreas were obtained at the LGSEO inclusion levels of 196.5 and 251 mg/kg, respectively. Inclusion of 100 to 300 mg/kg of LGSEO in the diet reduced the total Salmonella sp. bacterial count.
Research highlights: The use of 196.5 mg/kg of LGSEO in the diet of Japanese quail improved production performance and organ development and demonstrated potential antimicrobial capacity against Salmonella sp. bacteria. Due its pharmacological composition, LGSEO can potentially substitute to antimicrobials, because contains thymol and carvacrol as main active constituents.
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References
Andrade CYT, Andrade MA, Café MB, Stringhini JH, Mori A, Moraes DMC, Alcântara JB, Costa HX, 2012. Efeitos da Salmonella enteritidis experimentalmente inoculada na saúde gastrintestinal de perus. R Bras Zootec 41: 618-623. https://doi.org/10.1590/S1516-35982012000300021
AOAC, 2012. Official Methods of Analysis, 19th ed. Association of Official Analytical Chemists, Gaithersburg, MD, USA. 3390 pp.
Basmacioğlu Malayoğlu H, Baysal Ş, Misirlioğlu Z, Polat M, Yilmaz H, Turan N, 2010. Effects of oregano essential oil with or without feed enzymes on growth performance, digestive enzyme, nutrient digestibility, lipid metabolism and immune response of broilers fed on wheat-soybean meal diets. Br Poult Sci 51: 67-80. https://doi.org/10.1080/00071660903573702
Behnamifar A, Rahimi S, Karimi Torshizi MA, Hasanpour S, Mohammadzade Z, 2015. Effect of thyme, garlic and caraway herbal extracts on blood parameters productivity, egg quality, hatchability and intestinal bacterial population of laying Japanese quail. Iran J Vet Med 9: 179-187.
Bona TDMM, Pickler L, Miglino LB, Kuritza LN, Vasconcelos SP, Santin E, 2012. Óleo essencial de orégano, alecrim, canela e extrato de pimenta no controle de Salmonella, Eimeria e Clostridium em frangos de corte. Pesqui Vet Bras 32: 411-418. https://doi.org/10.1590/S0100-736X2012000500009
Brazilian Tables for Poultry and Swine, 2011. Food composition and nutritional requirements, 3rd ed. Imprensa Universitária, Viçosa, MG. 488 pp.
Brenes A, Roura E, 2010. Essential oils in poultry nutrition: Main effects and modes of action. Anim Feed Sci Tech 158: 1-4. https://doi.org/10.1016/j.anifeedsci.2010.03.007
Costa Júnior LM, Miller RJ, Alves PB, Blank AF, Li AY, León AAP, 2016. Acaricidal efficacies of Lippia gracilis essential oil and its phytochemicals against organophosphate-resistant and susceptible strains of Rhipicephalus (Boophilus) microplus. Vet Parasitol 228: 60-64. https://doi.org/10.1016/j.vetpar.2016.05.028
Cho JH, Kim HJ, Kim IH, 2014. Effects of phytogenic feed additive on growth performance, digestibility, blood metabolites, intestinal microbiota meat color and relative organ weight after oral challenge with Clostridium perfringens in broilers. Livest Sci 160: 82-88. https://doi.org/10.1016/j.livsci.2013.11.006
Daader HA, Al-Sagheer AA, Gabr HA, El-Moniem EAA, 2018. Alleviation of heat-stress-related physiological perturbations in growing rabbits using natural antioxidants. Span J Agric Res 16 (3): e0610. https://doi.org/10.5424/sjar/2018163-13184
Dantas LIS, Araujo GM, Ribeiro NM, Andrade VS, Oliveira FFM, Albuquerque CC, Rocha FAG, 2015. Antibacterial potential of Lippia gracilis Schauer essential oil against a Salmonella sp. strain isolated in Tilápia fillet-fish (Oreochromis Niloticus). Int J Sci Res 04: 500-503.
Dipineto L, Russo TP, Gargiulo A, Borrelli L, De Luca Bossa LM, Santaniello A, Buonocore P, Menna L.F, Fioretti A, 2014. Prevalence of enteropathogenic bacteria in common quail (Coturnix coturnix). Avian Pathol 43: 498-500. https://doi.org/10.1080/03079457.2014.966055
El-Hack MEA, Aahgoub SA, Alagawany M, Dharma K, 2015. Influences of dietary supplementation of antimicrobial cold pressed oils mixture on growth performance and intestinal microflora of growing Japanese quails. Int J Pharmacol 11: 689-696. https://doi.org/10.3923/ijp.2015.689.696
Franz C, Baser KHC, Windisch W, 2010. Essential oils and aromatic plants in animal feeding - A European perspective. A review. Flavour Fragr J 25: 327-340. https://doi.org/10.1002/ffj.1967
Giannenas IA, Papaneophytou CP, Tsalie E, Triantafillou E, Tontis D, Kontopidis GA, 2014. The effects of benzoic acid and essential oil compounds in combination with protease on the performance of chickens. J Anim Feed Sci 23: 73-81. https://doi.org/10.22358/jafs/65719/2014
Gul ST, Khan A, Ahmad M, Ahmad H, Saleemi MK, Naseem MN, Bilal M, 2018. Immuno-toxicological effects of different sub-lethal doses of thiamethoxam (TMX) in broiler birds. Toxin Rev 38: 1-6. https://doi.org/10.1080/15569543.2018.1435554
Guo X, Stedtfeld RD, Hedman H, Eiscenberg JNS, Truebac G, Yin D, Tiedje JM, Zhang L, 2018. Antibiotic resistome associated with small scale poultry production in rural Ecuador. Environ Sci Technol 52: 8165-8172. https://doi.org/10.1021/acs.est.8b01667
Horošová K, Bujňáková D, Kmeť V, 2006. Effect of oregano essential oil on chicken Lactobacilli and E. coli. Folia Microbiol 51: 278-280. https://doi.org/10.1007/BF02931812
Kheiri F, Faghani M, Landy N, 2018. Evaluation of thyme and ajwain as antibiotic growth promoter substitutions on growth performance, carcass characteristics and serum biochemistry in Japanese quails (Coturnix japonica). Anim Nutr 4: 79-83. https://doi.org/10.1016/j.aninu.2017.09.002
Koiyama NTG, Rosa AP, Padilha MTS, Boemo LS, Scher A, Melo AS, Fernandes MO, 2014. Desempenho e rendimento de carcaça de frangos de corte alimentados com mistura de aditivos fitogênicos na dieta. Pesqu Agropec Bras 49: 225-231. https://doi.org/10.1590/S0100-204X2014000300009
Lobo PDL, Fonteles CSR, Carvalho CBM, Nascimento DF, Fonseca SGC, Jamacaru FVF, Moraes MEA, 2011. Dose-response evaluation of a novel essential oil against Mutans streptococci in vivo. Ann Phytomed 18: 551-556. https://doi.org/10.1016/j.phymed.2010.10.018
Matshogo TB, Mlambo V, Marumel U, Sebola N, 2018. Growth performance, blood parameters, carcass characteristics and meat quality traits in Potchefstroom Koekoek chickens fed Lippia javanica leaf meal. Trop Anim Health Prod 45: 1-9. https://doi.org/10.1007/s11250-018-1620-9
Mnisi CM, Matshogo TB, Niekerk van R, Mlambo V, 2017. Growth performance, haemo-biochemical parameters and meat quality characteristics of male Japanese quails fed a Lippia javanica-based diet. S Afr J Anim Sci 47: 661-671. https://doi.org/10.4314/sajas.v47i5.9
Mpofu DA, Marume U, Mlambo V, Hugo A, 2016. The effect of Lippia javanica dietary inclusion on growth performance, carcass characteristics and fatty acid profiles of broiler chickens. Anim Nutr 2: 160-167. https://doi.org/10.1016/j.aninu.2016.05.003
Oliveira DR, Leitão GG, Bizzo HR, Lopes D, Alviano DS, Alviano CS, Leitão SG, 2007. Chemical and antimicrobial analyses of essential oil of Lippia origanoides H.B.K. Food Chem 101: 246-240. https://doi.org/10.1016/j.foodchem.2006.01.022
Patel JB, Cockerill FR, Bradford PA, 2015. Performance standard for antibacterial susceptibility testing; Twenty-Fifth Informational Supplement, publication M100-S25. Clinical and Laboratory Standard Institute 25: 29-50. https://doi.org/10.7748/ns.29.50.25.s27
Pereira NLF, Aquino PEA, Jos Júnior GAS, Cristo JAS, Vieira Filho MA, Moura FF, et al., 2017. In vitro evaluation of the antibacterial potential and modification of antibiotic activity of the Eugenia uniflora L. essential oil in association with led lights. Micro Pathog 110: 512-518. https://doi.org/10.1016/j.micpath.2017.07.048
Ramos RS, Rodrigues ABL, Farias ALF, Simões RC, Pinheiro MT, Ferreira RMA, et al., 2017. Chemical composition and in vitro antioxidant, cytotoxic, antimicrobial, and larvicidal activities of the essential oil of mentha piperita l. (lamiaceae). Sci World J: Article ID 4927214. https://doi.org/10.1155/2017/4927214
Rocha GF, Del Vesco AP, Santana TP, Santos TS, Cerqueira AS, Zancanela VT, Fernandes RPM, Oliveira Júnior GM, 2020. Lippia gracilis Schauer essential oil as a growth promoter for Japanese quail. Anim 14: 2023-2031. https://doi.org/10.1017/S1751731120000816
Santos MM, Peixoto AR, Pessoa ES, Nepa HBS, Paz CD, De Souza AVV, 2014. Estudos dos constituintes químicos e atividade antibacteriana do óleo essencial de Lippia gracilis a Xanthomonas campestris pv. viticola "in vitro". Summa Phytopathol 40: 277-280. https://doi.org/10.1590/0100-5405/1958
Santos CP, Pinto JAO, Santos CA, Cruz EMO, Arrigoni-Blank MF, Andrade TM, Santos DA, Alves PB, Blank AF, 2016. Harvest time and geographical origin affect the essential oil of Lippia gracilis Schauer. J Crop Prot 79: 205-210. https://doi.org/10.1016/j.indcrop.2015.11.015
Sarrazin SLF, Oliveira RB, Barata LES, Mourao RHV, 2012. Chemical composition and antimicrobial activity of the essential oil of Lippia grandis Schauer (Verbenaceae) from the western Amazon. Food Chem 138: 1474-1478. https://doi.org/10.1016/j.foodchem.2012.03.058
SAS, 2002. SAS User's guide version 9.1: Statistics. SAS Inst. Inc., Cary, NC, USA.
Silva RS, Oliveira MMG, Melo JO, Blank AF, Corrêa CB, Scher R, Fernandes RPM, 2019. Antimicrobial activity of Lippia gracilis essential oils on the plant pathogen Xanthomonas campestris pv. campestris and their effect on membrane integrity. Pestic Biochem Phys 160: 40-48. https://doi.org/10.1016/j.pestbp.2019.06.014
Wang TH, Hsia SM, Wu CH, Ko SY, Chen MY, Shih YH, Shieh TM, Chuang LC, Wu CY, 2016. Evaluation of the antibacterial potential of liquid and vapor phase phenolic essential oil compounds against oral microorganisms. Plos One 11: e0163147. https://doi.org/10.1371/journal.pone.0163147
Windisch W, Schedle K, Plitzner C, Kroismayr A, 2008. Use of phytogenic products as feed additives for swine and poultry. J Anim Sci 86: 140-148. https://doi.org/10.2527/jas.2007-0459
Zhai H, Liu H, Wang S, Wu J, Kluenter AM, 2018. Potential of essential oils for poultry and pigs. Anim Nutr 4: 179-186. https://doi.org/10.1016/j.aninu.2018.01.005
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